دورية أكاديمية
أعرض في EDS

Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity.

التفاصيل البيبلوغرافية
العنوان: Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity.
المؤلفون: Shafi KM; National Centre for Biological Sciences (TIFR), GKVK campus, Bangalore, India.; Centre for Ayurveda Biology and Holistic Nutrition, The University of Trans-Disciplinary Health Sciences & Technology (TDU), Yelahanka, Bangalore, 560064, Karnataka, India., Sajeevan RS; National Centre for Biological Sciences (TIFR), GKVK campus, Bangalore, India.; Swedish University of Agricultural Sciences, SE-23422, Lomma, Sweden., Kouser S; Centre for Ayurveda Biology and Holistic Nutrition, The University of Trans-Disciplinary Health Sciences & Technology (TDU), Yelahanka, Bangalore, 560064, Karnataka, India., Vishnuprasad CN; Centre for Ayurveda Biology and Holistic Nutrition, The University of Trans-Disciplinary Health Sciences & Technology (TDU), Yelahanka, Bangalore, 560064, Karnataka, India. drcnvp@gmail.com., Sowdhamini R; National Centre for Biological Sciences (TIFR), GKVK campus, Bangalore, India. mini@ncbs.res.in.; Institute of Bioinformatics and Applied Biotechnology, Bangalore, 560100, India. mini@ncbs.res.in.
المصدر: BMC plant biology [BMC Plant Biol] 2022 Dec 02; Vol. 22 (1), pp. 561. Date of Electronic Publication: 2022 Dec 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Moringa*, Hypoglycemic Agents/pharmacology ; Quercetin ; Chlorogenic Acid ; Gene Expression Profiling ; Benzylamines ; Plant Extracts/pharmacology
مستخلص: Background: Moringa concanensis Nimmo (MC), a plant that resembles Moringa oleifera Lam. (MO), has less scientific information but has traditionally been used as a medicinal plant. Moringa species have long been known for their medicinal qualities, which include antioxidant, anti-inflammatory, anticancer, and antihyperglycemic effects. We investigated the antidiabetic potential of MC and MO species in this study by using transcriptome profiling, metabolite analysis, and in vitro assay studies.
Results: Our transcriptome analysis revealed the expression of enzymes involved in the biosynthesis of quercetin, chlorogenic acid, and benzylamine, all of which have previously been shown to have antidiabetic activity. We compared the expression patterns of five different tissues from MC and MO and it was found that the key enzymes involved in the biosynthesis of these compounds were highly expressed in leaf tissue. The expression estimated by MC transcriptome data in different tissues was verified using RT-qPCR analysis. The amount of these compounds was further quantified in the crude leaf extract of both species and found that MC had a higher abundance of quercetin and chlorogenic acid than MO. The crude leaf extract from both MC and MO were further tested in vitro, and the results demonstrated strong inhibitory activity for α-glucosidase and DPP-IV enzymes. Our findings suggest that compounds in leaf tissue, such as quercetin, benzylamine, and chlorogenic acid, could play a significant role in this antidiabetic activity. In addition, when comparing MO plants, we found that MC had a slightly higher effect in expression, abundance, and inhibitory activity.
Conclusions: This study presents the first report of MC transcriptome data, as well as a comparison of its anti-diabetic activity to MO. Our analysis discussed the significance of leaf tissue in antidiabetic activity compared to other tissues of both species. Overall, this study not only provides transcriptome resources for Moringa species, but also sheds light on antidiabetic potential of both species.
(© 2022. The Author(s).)
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معلومات مُعتمدة: SB/S2/JC-071/2015 JC Bose fellowship Science and Engineering Research Board, Department of Science and Technology, India; BT/PR40187/BTIS/137/9/2021, Bioinformatics Centre Grant Department of Biotechnology, India; IBAB/MSCB/182/2022 Mazumdar-Shaw Chair in Computational Biology Institute of Bioinformatics and Applied Biotechnology, India
فهرسة مساهمة: Keywords: Diabetes; Enzyme inhibition; Moringaceae; RNA-seq; Secondary metabolites; Transcript expression
المشرفين على المادة: 0 (Hypoglycemic Agents)
9IKM0I5T1E (Quercetin)
318ADP12RI (Chlorogenic Acid)
A1O31ROR09 (benzylamine)
0 (Benzylamines)
0 (Plant Extracts)
تواريخ الأحداث: Date Created: 20221202 Date Completed: 20221206 Latest Revision: 20240908
رمز التحديث: 20240909
مُعرف محوري في PubMed: PMC9717441
DOI: 10.1186/s12870-022-03938-6
PMID: 36460949
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2229
DOI:10.1186/s12870-022-03938-6