دورية أكاديمية
Environmental concentrations of benzophenone-3 disturbed lipid metabolism in the liver of clown anemonefish (Amphiprion ocellaris).
| العنوان: | Environmental concentrations of benzophenone-3 disturbed lipid metabolism in the liver of clown anemonefish (Amphiprion ocellaris). |
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| المؤلفون: | Zhang YK; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China., Ke HY; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China., Qin YQ; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China., Ju HY; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China., Chen YM; College of Ecology and Environment Hainan University, Haikou, Hainan, 570228, China., Lin F; College of Ecology and Environment Hainan University, Haikou, Hainan, 570228, China., Zhang JL; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China., Diao XP; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China. Electronic address: diaoxip@hainanu.edu.cn. |
| المصدر: | Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2023 Jan 15; Vol. 317, pp. 120792. Date of Electronic Publication: 2022 Dec 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8804476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6424 (Electronic) Linking ISSN: 02697491 NLM ISO Abbreviation: Environ Pollut Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Barking, Essex, England : Elsevier Applied Science Publishers, c1987- |
| مواضيع طبية MeSH: | Lipid Metabolism* , Perciformes*/metabolism, Animals ; Liver/metabolism ; Benzophenones |
| مستخلص: | Benzophenone-3 (BP-3) often used as a UV filter in various products and an endocrine disruptor. In this work, we exposed the clown anemonefish to 10 μg/L and 50 μg/L BP-3 for 7 and 14 days. Liver histological, biochemical analysis, and transcriptome sequencing were used to explore the mechanism of the lipid metabolism disorder in the liver of three-month-old clown anemonefish treated with BP-3. The histological and biochemical analysis showed that BP-3 induces morphological changes and lipid droplet accumulation, and the lipid content, lipase, and antioxidant enzyme activity were abnormal. After treatment with 10 μg/L and 50 μg/L BP-3 for 7 days, the transcriptome analysis further demonstrated that the KEGG analysis revealed that the differentially expressed genes (DEGs) were mainly associated with fat digestion and absorption, PPAR signaling pathway, circadian rhythm, and mineral absorption pathways; After 10 μg/L and 50 μg/L of BP-3 exposure for 14 days, the KEGG analysis were mainly associated with circadian rhythm, circadian rhythm-fly, protein processing in the endoplasmic reticulum, and beta-alanine metabolism pathways. Several key genes were involved in the process of liver lipid metabolism, including CD36, APoA-Ⅰ, FABP, LPL, ACS, and PEPCK. The qRT-PCR validation results showed that eight genes (CYP8B1, FABP1, LPL, MGAT, PEPCK, PER1, PSMB4, PSME2) were significantly down-regulated, and the other two genes (Fbxl3, RXR) were significantly up-regulated after 7 days of BP-3 exposure. Similarly, eleven genes (AMPK, ARNTL, Bmal1, CASP3, CYC, CYP2J, CYP2U1, GSK3A, PEPCK, RAC1, RORA) were significantly up-regulated, and the other four genes (NR1D1, PER1, PTGDS, HLF) were significantly down-regulated after 14 days of BP-3 exposure. In conclusion, our results elucidate the physiological and molecular responses to BP-3 exposure in the liver lipid metabolism of clown anemonefish, and these findings reveal that the regulation of lipid metabolism is disturbed when clown anemonefish is exposed to UV filters. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Coral reef fish; Endocrine disrupting compounds; Lipid metabolism; Liver toxicity; UV filters |
| المشرفين على المادة: | 95OOS7VE0Y (oxybenzone) 0 (Benzophenones) |
| تواريخ الأحداث: | Date Created: 20221206 Date Completed: 20230105 Latest Revision: 20230111 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.envpol.2022.120792 |
| PMID: | 36473638 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6424 |
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| DOI: | 10.1016/j.envpol.2022.120792 |