دورية أكاديمية
أعرض في EDS

In vitro production of infectious Plasmodium falciparum sporozoites.

التفاصيل البيبلوغرافية
العنوان: In vitro production of infectious Plasmodium falciparum sporozoites.
المؤلفون: Eappen AG; Sanaria, Rockville, MD, USA., Li T; Sanaria, Rockville, MD, USA., Marquette M; Sanaria, Rockville, MD, USA., Chakravarty S; Sanaria, Rockville, MD, USA., Kc N; Sanaria, Rockville, MD, USA.; Protein Potential, Rockville, MD, USA., Zanghi G; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA., Hoffman BU; Columbia University Irving Medical Center, New York, NY, USA.; Department of Medicine, UCSF, San Francisco, CA, USA., Hettiarachchi H; Sanaria, Rockville, MD, USA.; Heritage College of Osteopathic Medicine, Ohio University, Dublin, OH, USA., Patil A; Sanaria, Rockville, MD, USA., Abebe Y; Sanaria, Rockville, MD, USA., Tran C; Sanaria, Rockville, MD, USA., Yossef AA; Sanaria, Rockville, MD, USA., McWilliams I; Sanaria, Rockville, MD, USA., Morrison RD; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA., Rathakrishnan A; Sanaria, Rockville, MD, USA., Inbar E; Sanaria, Rockville, MD, USA., Aly ASI; Sanaria, Rockville, MD, USA., De La Vega P; Sanaria, Rockville, MD, USA., Belmonte M; Malaria Department, Naval Medical Research Center, Silver Spring, MD, USA.; Henry M. Jackson Foundation, Bethesda, MD, USA., Sedegah M; Malaria Department, Naval Medical Research Center, Silver Spring, MD, USA., Wai T; Sanaria, Rockville, MD, USA.; Protein Potential, Rockville, MD, USA., Campo JJ; Antigen Discovery Incorporated (ADI), Irvine, CA, USA., King H; Institute for Bioscience and Biotechnology Research, Rockville, MD, USA., Kappe SHI; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Department of Pediatrics, University of Washington, Seattle, WA, USA.; Department of Global Health, University of Washington, Seattle, WA, USA., Li M; Sanaria, Rockville, MD, USA.; Protein Potential, Rockville, MD, USA., Billingsley PF; Sanaria, Rockville, MD, USA., Sim BKL; Sanaria, Rockville, MD, USA.; Protein Potential, Rockville, MD, USA., Hoffman SL; Sanaria, Rockville, MD, USA. slhoffman@sanaria.com.
المصدر: Nature [Nature] 2022 Dec; Vol. 612 (7940), pp. 534-539. Date of Electronic Publication: 2022 Dec 07.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, P.H.S.; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
أسماء مطبوعة: Publication: Basingstoke : Nature Publishing Group
Original Publication: London, Macmillan Journals ltd.
مواضيع طبية MeSH: Plasmodium falciparum*/growth & development , Sporozoites*/growth & development , Sporozoites*/pathogenicity, Animals ; Humans ; Mice ; Culicidae/parasitology ; Malaria/parasitology ; Malaria/prevention & control ; Malaria Vaccines/biosynthesis ; Malaria Vaccines/chemistry ; Malaria, Falciparum/parasitology ; Hepatocytes/parasitology ; Liver/parasitology ; Merozoite Surface Protein 1 ; Erythrocytes/parasitology ; In Vitro Techniques
مستخلص: An effective vaccine is needed for the prevention and elimination of malaria. The only immunogens that have been shown to have a protective efficacy of more than 90% against human malaria are Plasmodium falciparum (Pf) sporozoites (PfSPZ) manufactured in mosquitoes (mPfSPZ) 1-7 . The ability to produce PfSPZ in vitro (iPfSPZ) without mosquitoes would substantially enhance the production of PfSPZ vaccines and mosquito-stage malaria research, but this ability is lacking. Here we report the production of hundreds of millions of iPfSPZ. iPfSPZ invaded human hepatocytes in culture and developed to mature liver-stage schizonts expressing P. falciparum merozoite surface protein 1 (PfMSP1) in numbers comparable to mPfSPZ. When injected into FRGhuHep mice containing humanized livers, iPfSPZ invaded the human hepatocytes and developed to PfMSP1-expressing late liver stage parasites at 45% the quantity of cryopreserved mPfSPZ. Human blood from FRGhuHep mice infected with iPfSPZ produced asexual and sexual erythrocytic-stage parasites in culture, and gametocytes developed to PfSPZ when fed to mosquitoes, completing the P. falciparum life cycle from infectious gametocyte to infectious gametocyte without mosquitoes or primates.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)
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معلومات مُعتمدة: R43 AI085740 United States AI NIAID NIH HHS; 2R44AI085740-03A1 United States AI NIAID NIH HHS; 2R44AI085740-06A1 United States AI NIAID NIH HHS; W81XWH-16-2-0025 US Department of Defense
المشرفين على المادة: 0 (Malaria Vaccines)
0 (Merozoite Surface Protein 1)
تواريخ الأحداث: Date Created: 20221208 Date Completed: 20230111 Latest Revision: 20230201
رمز التحديث: 20231215
DOI: 10.1038/s41586-022-05466-7
PMID: 36477528
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-4687
DOI:10.1038/s41586-022-05466-7