دورية أكاديمية
CDT1 is the major functional regulatory subunit of the pre-replication complex in zygotes.
| العنوان: | CDT1 is the major functional regulatory subunit of the pre-replication complex in zygotes. |
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| المؤلفون: | Li C; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.; University of Chinese Academy of Sciences, Beijing, China., Tan YP; Fertility Preservation Lab, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, China., Ma XS; Reproductive Genetics Department, The Affiliated Tai'an City Central Hospital of Qingdao University, Taian, China., Wang ZB; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.; University of Chinese Academy of Sciences, Beijing, China., Meng TG; Fertility Preservation Lab, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, China., Sun QY; Fertility Preservation Lab, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, China. |
| المصدر: | Cell proliferation [Cell Prolif] 2023 Mar; Vol. 56 (3), pp. e13377. Date of Electronic Publication: 2022 Dec 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications Country of Publication: England NLM ID: 9105195 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2184 (Electronic) Linking ISSN: 09607722 NLM ISO Abbreviation: Cell Prolif Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Oxford, England] : Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications, 1991- |
| مواضيع طبية MeSH: | Zygote*/metabolism , Cell Cycle Proteins*/metabolism, DNA Replication ; Cell Cycle ; DNA |
| مستخلص: | Pre-replication complex (pre-RC) is critical for DNA replication initiation. CDT1 and MCM2 are the subunits of pre-RC, and proper regulation of CDT1 and MCM2 are necessary for DNA replication and cell proliferation. The present study aimed to explore the role of CDT1 and MCM2 in oocyte meiotic maturation and early embryonic development. The depletion and overexpression of Cdt1 and Mcm2 in oocyte and zygote were achieved by microinjecting specific siRNA and mRNA to explored their functions in oocyte meiotic maturation and embryonic development. Then, we examined the effect of CDT1 and MCM2 on other signal pathways by immunostaining the expression of related maker genes. We showed that neither depletion nor overexpression of Cdt1 affected oocyte meiotic progressions. The CDT1 was degraded in S phase and remained at a low level in G2 phase of zygote. Exogenous expression of Cdt1 in G2 phase led to embryo attest at zygote stage. Mechanistically, CDT1 overexpression induced DNA re-replication and thus DNA damage check-point activation. Protein abundance of MCM2 was stable throughout the cell cycle, and embryos with overexpressed MCM2 could develop to blastocysts normally. Overexpression or depletion of Mcm2 also had no effect on oocyte meiotic maturation. Our results indicate that pre-RC subunits CDT1 and MCM2 are not involved in oocyte meiotic maturation. In zygote, CDT1 but not MCM2 is the major regulator of DNA replication in a cell cycle dependent manner. Furthermore, its' degradation is essential for zygotes to prevent from DNA re-replication in G2 stage. (© 2022 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | 2021YFA1100302 National R&D Project, China; 32570854 National Natural Science Foundation of China; 2021YFA1100302 the National R&D Project, China; 32570854 the National Natural Science Foundation of China |
| المشرفين على المادة: | 0 (Cell Cycle Proteins) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20221208 Date Completed: 20230303 Latest Revision: 20230304 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9977660 |
| DOI: | 10.1111/cpr.13377 |
| PMID: | 36479743 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2184 |
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| DOI: | 10.1111/cpr.13377 |