دورية أكاديمية
Equine peripheral blood CD14 + monocyte-derived macrophage in-vitro characteristics after GM-CSF pretreatment and LPS+IFN-γ or IL-4+IL-10 differentiation.
العنوان: | Equine peripheral blood CD14 + monocyte-derived macrophage in-vitro characteristics after GM-CSF pretreatment and LPS+IFN-γ or IL-4+IL-10 differentiation. |
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المؤلفون: | Bowlby CM; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, United States., Purmessur D; Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, United States., Durgam SS; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, United States. Electronic address: durgam.3@osu.edu. |
المصدر: | Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2023 Jan; Vol. 255, pp. 110534. Date of Electronic Publication: 2022 Dec 07. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Scientific Country of Publication: Netherlands NLM ID: 8002006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2534 (Electronic) Linking ISSN: 01652427 NLM ISO Abbreviation: Vet Immunol Immunopathol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Amsterdam] Elsevier Scientific. |
مواضيع طبية MeSH: | Granulocyte-Macrophage Colony-Stimulating Factor*/pharmacology , Granulocyte-Macrophage Colony-Stimulating Factor*/metabolism , Lipopolysaccharides*/pharmacology, Animals ; Horses ; Interleukin-10/metabolism ; Interleukin-4/metabolism ; Monocytes ; Macrophages/metabolism ; Cell Differentiation ; Interferon-gamma/metabolism ; Cells, Cultured |
مستخلص: | Macrophages are a heterogeneous population of immune cells that exhibit dynamic plasticity, polarize into inflammatory or regulatory/pro-resolving macrophages, and influence the healing tissue microenvironment. This study evaluated the in-vitro morphological, proliferative, cell surface marker expression and cytokine/soluble factor secretion characteristics of control, GM-CSF pretreated and inflammatory (LPS+IFN-γ) and regulatory (IL-4 + IL-10) differentiated equine CD14 + monocyte-derived macrophages. Phase contrast microscopy demonstrated that LPS+IFN-γ-primed macrophages exhibited a rounded, granular morphology, whereas IL-4 +IL-10-primed macrophages were elongated with a spindle-shaped morphology. GM-CSF enhanced the proliferation rate of monocytes/macrophages during adherent in-vitro culture. Flow cytometry analysis showed that GM-CSF alone and GM-CSF pretreatment with LPS+IFN-γ or IL-4 +IL-10 priming increased CD86 immunopositivity by 2-fold (p = 0.6); and CD206 immunopositivity remained unchanged. GM-CSF pretreatment and subsequent priming with LPS and IFN-γ yielded inflammatory macrophages that secrete significantly increased quantities of IL-1β compared to control (p = 0.012) and IL-4 +IL-10-primed (p = 0.0047) macrophages. GM-CSF pretreatment followed by both LPS + IFN-γ and IL-4 + IL-10 priming significantly increased IL-1Ra secretion by 6-fold (p < 0.05). There were no differences in TGFβ-1 secretion among control, LPS+IFN-γ or IL-4 + IL-10 primed macrophages (p = 0.85). All groups contained an average of 643 ± 51.5 pg/mL of TGFβ1. Among the culture conditions evaluated, IL-4 +IL-10 priming for 24 h after 6 days of adherent culture yielded macrophages that were the least inflammatory compared to GM-CSF pretreated and LPS+IFN-γ or IL-4 +IL-10-primed macrophages. These results provide a basis for subsequent in-vitro and in-vivo studies that investigate macrophage-tissue cell interactions and related biological mechanisms relevant to the field of immunomodulatory approaches for enhancing tissue healing. Competing Interests: Declaration of Competing Interest The authors do not have any conflicts of interest. (Copyright © 2022. Published by Elsevier B.V.) |
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معلومات مُعتمدة: | KL2 TR002734 United States TR NCATS NIH HHS |
فهرسة مساهمة: | Keywords: Cytokine; Equine; Immunomodulation; Macrophage; Monocyte |
المشرفين على المادة: | 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor) 0 (Lipopolysaccharides) 130068-27-8 (Interleukin-10) 207137-56-2 (Interleukin-4) 82115-62-6 (Interferon-gamma) |
تواريخ الأحداث: | Date Created: 20221211 Date Completed: 20230102 Latest Revision: 20240102 |
رمز التحديث: | 20240102 |
مُعرف محوري في PubMed: | PMC9807231 |
DOI: | 10.1016/j.vetimm.2022.110534 |
PMID: | 36502640 |
قاعدة البيانات: | MEDLINE |
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