دورية أكاديمية
Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions.
| العنوان: | Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions. |
|---|---|
| المؤلفون: | Van AL; Université de Rennes 1, CNRS, UMR 6553 Ecobio, Rennes, France., Duhamel M; Université de Rennes 1, CNRS, UMR 6553 Ecobio, Rennes, France.; Wageningen Plant Research, Biointeractions and Plant Health, Wageningen, The Netherlands., Quaiser A; Université de Rennes 1, CNRS, UMR 6553 Ecobio, Rennes, France., Vandenkoornhuyse P; Université de Rennes 1, CNRS, UMR 6553 Ecobio, Rennes, France. philippe.vandenkoornhuyse@univ-rennes1.fr. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2605, pp. 169-186. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | RNA* , Mycorrhizae*/genetics, Isotope Labeling/methods ; Isotopes ; RNA, Ribosomal/genetics ; Plants/genetics ; Plants/microbiology ; Carbon Isotopes |
| مستخلص: | The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Carbon thirteen; Carbon transfer; Fungal plant symbiont; RNA; Stable-isotope probing (SIP); qRT-PCR |
| المشرفين على المادة: | 63231-63-0 (RNA) 0 (Isotopes) 0 (RNA, Ribosomal) 0 (Carbon Isotopes) |
| تواريخ الأحداث: | Date Created: 20221215 Date Completed: 20221219 Latest Revision: 20230304 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-0716-2871-3_9 |
| PMID: | 36520394 |
| قاعدة البيانات: | MEDLINE |
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