دورية أكاديمية
Behavior of the renal kallikrein in spontaneously hypertensive rats: Influence of sexual hormones and aldosterone-sensitive distal nephron ion channels.
| العنوان: | Behavior of the renal kallikrein in spontaneously hypertensive rats: Influence of sexual hormones and aldosterone-sensitive distal nephron ion channels. |
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| المؤلفون: | Azurmendi PJ; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina. Electronic address: azurmendi.pablo@lanari.uba.ar., Toro AR; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Celía AF; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina., Guevara D; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina., Solerno MR; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina., Di Ciano LA; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina., Toledo JE; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina., Ibarra FR; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina; Universidad de Buenos Aires, Departamento de Ciencias Fisiológicas, Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO Houssay)-CONICET, Facultad de Medicina, Buenos Aires, Argentina., Arrizurieta EE; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Oddo EM; Universidad de Buenos Aires, Laboratorio de Nefrología Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas A. Lanari, Buenos Aires, Argentina. |
| المصدر: | Peptides [Peptides] 2023 Feb; Vol. 160, pp. 170925. Date of Electronic Publication: 2022 Dec 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Inc Country of Publication: United States NLM ID: 8008690 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5169 (Electronic) Linking ISSN: 01969781 NLM ISO Abbreviation: Peptides Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Elsevier Science Inc. Original Publication: Fayetteville, N. Y., Ankho International. |
| مواضيع طبية MeSH: | Aldosterone*/metabolism , Hypertension*/metabolism, Male ; Rats ; Female ; Animals ; Rats, Inbred SHR ; Receptors, Mineralocorticoid/metabolism ; Kallikreins/genetics ; Kallikreins/metabolism ; Kidney/metabolism ; Nephrons/metabolism ; Sodium/metabolism ; Ion Channels/metabolism ; Epithelial Sodium Channels/genetics ; Epithelial Sodium Channels/metabolism |
| مستخلص: | The renal kallikrein-kinin system (RKKS) has been related to blood pressure control and sodium and water balance. We have previously shown that female spontaneously hypertensive rats (SHR) have high urinary kallikrein activity (UKa) and lower blood pressure (BP) than males whereas ovariectomy stimulates UKa and diminishes BP. We also showed that high K + intake and prepuberal gonadectomy (Gx) diminish BP with a concomitant increase in UKa and plasma aldosterone levels. Since kallikrein co-localize in the same distal nephron segments of aldosterone effectors, we explored the effect of pharmacological blockage of aldosterone receptor, epithelial Na + (ENaC) and the rectifying outer medulla K + (ROMK) channels in different gonad contexts on the gene expression, renal tissue content and urine release of kallikrein. Klk1 gene expression was determined by real-time PCR and enzymatic activity of kallikrein by the amidolytic method. We found that the inhibition of the aldosterone receptor by spironolactone increases kallikrein renal tissue storage and decreases its urinary activity, especially in Gx rats. Moreover, ENaC blockade by benzamil increases the renal content of kallikrein without affecting synthesis or excretion, especially in females and Gx animals, while the inhibition of ROMK by glibenclamide increases the synthesis and renal content of kallikrein only in intact male animals. We concluded that RKKS regulation showed sexual dimorphism and seemed to be modulated by sex hormones throughout a process involving aldosterone and the aldosterone-sensitive ion channels.. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aldosterone receptor; Epithelial sodium channel; Gonadectomy; Rectifying outward potassium channel; Renal kallikrein |
| المشرفين على المادة: | 4964P6T9RB (Aldosterone) 0 (Receptors, Mineralocorticoid) EC 3.4.21.- (Kallikreins) 9NEZ333N27 (Sodium) 0 (Ion Channels) 0 (Epithelial Sodium Channels) |
| تواريخ الأحداث: | Date Created: 20221222 Date Completed: 20230120 Latest Revision: 20230503 |
| رمز التحديث: | 20230504 |
| DOI: | 10.1016/j.peptides.2022.170925 |
| PMID: | 36549423 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-5169 |
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| DOI: | 10.1016/j.peptides.2022.170925 |