A New Gnotobiotic Pig Model of P[6] Human Rotavirus Infection and Disease for Preclinical Evaluation of Rotavirus Vaccines.

التفاصيل البيبلوغرافية
العنوان:	A New Gnotobiotic Pig Model of P[6] Human Rotavirus Infection and Disease for Preclinical Evaluation of Rotavirus Vaccines.
المؤلفون:	Nyblade C; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Hensley C; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Parreño V; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA.; Incuinta, IVIT, INTA-Conicet, Hurlingham, Provincia de Buenos Aires 1686, Argentina., Zhou P; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Frazier M; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Frazier A; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Ramesh A; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Lei S; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA., Degiuseppe JI; Laboratory of Viral Gastroenteritis, National Institute for Infectious Diseases (INEI-ANLIS 'Dr. Carlos G. Malbrán'), Ciudad Autónoma de Buenos Aires 1281, Argentina., Tan M; Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA., Yuan L; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA.
المصدر:	Viruses [Viruses] 2022 Dec 15; Vol. 14 (12). Date of Electronic Publication: 2022 Dec 15.
نوع المنشور:	Journal Article; Research Support, N.I.H., Extramural
اللغة:	English
بيانات الدورية:	Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH:	Rotavirus Infections/prevention & control , Rotavirus Infections/veterinary , Rotavirus* , Rotavirus Vaccines*, Infant ; Child ; Infant, Newborn ; Humans ; Animals ; Swine ; Child, Preschool ; Feces ; Germ-Free Life
مستخلص:	Human rotavirus (HRV) is a leading cause of gastroenteritis in children under 5 years of age. Licensed vaccines containing G1P[8] and G1-4P[8] strains are less efficacious against newly emerging P[6] strains, indicating an urgent need for better cross protective vaccines. Here, we report our development of a new gnotobiotic (Gn) pig model of P[6] HRV infection and disease as a tool for evaluating potential vaccine candidates. The Arg HRV (G4P[6]) strain was derived from a diarrheic human infant stool sample and determined to be free of other viruses by metagenomic sequencing. Neonatal Gn pigs were orally inoculated with the stool suspension containing 5.6 × 10 ⁵ fluorescent focus units (FFU) of the virus. Small and large intestinal contents were collected at post inoculation day 2 or 3. The virus was passaged 6 times in neonatal Gn pigs to generate a large inoculum pool. Next, 33-34 day old Gn pigs were orally inoculated with 10 ^-2 , 10 ³ , 10 ⁴ , and 10 ⁵ FFU of Arg HRV to determine the optimal challenge dose. All pigs developed clinical signs of infection, regardless of the inoculum dose. The optimal challenge dose was determined to be 10 ⁵ FFU. This new Gn pig model is ready to be used to assess the protective efficacy of candidate monovalent and multivalent vaccines against P[6] HRV.
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معلومات مُعتمدة:	R56 AI148426 United States AI NIAID NIH HHS
فهرسة مساهمة:	Keywords: P[6] genotype; diarrhea; gnotobiotic pig model; human rotavirus; vaccine evaluation
المشرفين على المادة:	0 (Rotavirus Vaccines)
تواريخ الأحداث:	Date Created: 20221223 Date Completed: 20221226 Latest Revision: 20230711
رمز التحديث:	20240628
مُعرف محوري في PubMed:	PMC9784283
DOI:	10.3390/v14122803
PMID:	36560807
قاعدة البيانات:	MEDLINE

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تدمد:	1999-4915
DOI:	10.3390/v14122803