دورية أكاديمية
[miR-181c promotes the migration and angiogenesis of human A549 cells via targeting inhibition of reversion-inducing cysteine-rich protein with Kazal motifs (RECK)].
العنوان: | [miR-181c promotes the migration and angiogenesis of human A549 cells via targeting inhibition of reversion-inducing cysteine-rich protein with Kazal motifs (RECK)]. |
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المؤلفون: | Liu C; Laboratory of Microbiology and Immunology, Tumor Immunology Laboratory, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China., Zhao F; Tumor Immunology Laboratory, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China., Sun P; Laboratory of Microbiology and Immunology, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China., Han W; Laboratory of Microbiology and Immunology, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China., Hong M; Laboratory of Microbiology and Immunology, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China., Wu J; Tumor Immunology Laboratory, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China., Zhang X; Laboratory of Microbiology and Immunology, Tumor Immunology Laboratory, College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010059, China. *Corresponding author, E-mail: zhxuan024@163.com. |
المصدر: | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2022 Dec; Vol. 38 (12), pp. 1084-1090. |
نوع المنشور: | English Abstract; Journal Article |
اللغة: | Chinese |
بيانات الدورية: | Publisher: Xi bao yu fen zi mian yi xue za zhi bian ji bu Country of Publication: China NLM ID: 101139110 Publication Model: Print Cited Medium: Print ISSN: 1007-8738 (Print) Linking ISSN: 10078738 NLM ISO Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Xiʹan : Xi bao yu fen zi mian yi xue za zhi bian ji bu |
مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Lung Neoplasms*/genetics , Lung Neoplasms*/metabolism, Humans ; Matrix Metalloproteinase 2/genetics ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/genetics ; Matrix Metalloproteinase 9/metabolism ; Vascular Endothelial Growth Factor A/genetics ; Cysteine ; Kazal Motifs ; A549 Cells ; Endothelial Cells/metabolism ; GPI-Linked Proteins/genetics ; GPI-Linked Proteins/metabolism ; Cell Movement/genetics |
مستخلص: | Objective To investigate the impact of miR-181c on migration and angiogenesis of lung cancer cells. Methods The Oncomine platform, UALCAN was used to analyze the differential expression of miR-181c and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in lung cancer obtained from the Cancer Genome Atlas (TCGA) database. The targeting relationship between miR-181c on RECK gene was predicted using Targetscan software. miR-181c mimic, inhibitor and negative control were introduced into A549 cells respectively. After transfection, the real-time quantitative PCR was used to detect the relative expressions of miR-181c and RECK mRNA, and Western blot analysis was used to detect the expression levels of RECK, matrix metalloproteinase 2 (MMP2) and MMP9 proteins. Transwell TM assay was performed to analyze the cell migration ability. The secretion of vascular endothelial growth factor (VEGF)-A in the cell culture supernatant was analyzed by using ELISA. Human umbilical vein endothelial cells (HUVECs) were treated with the culture supernatant, then in vitro tubule formation assay was carried out to evaluate the angiogenesis ability. The targeting correlation between miR-181c and RECK was validated by double luciferase reporter gene assay. Results UALCAN analysis displayed that the expression of miR-181c was significantly higher and RECK expression was significantly lower in lung cancer tissues compared to that in normal tissues. Targetscan prediction showed that there was a miR-181c binding site in the 3'-untranslated region (3' UTR) of RECK gene. miR-181c could downregulate the expression of RECK, increase the expressions of MMP2 and MMP9, and promote the A549 cell migration. ELISA and tubule formation assay showed that miR-181c could induce the secretion of VEGF-A in A549 cells and enhance the ability of HUVECs differentiae into tubules. The double luciferase reporter gene assay confirmed that RECK was the direct regulation target of miR-181c. Conclusion miR-181c promotes the migration and angiogenesis of human A549 cells by directly targeting RECK. |
المشرفين على المادة: | EC 3.4.24.24 (Matrix Metalloproteinase 2) 0 (MicroRNAs) EC 3.4.24.35 (Matrix Metalloproteinase 9) 0 (Vascular Endothelial Growth Factor A) K848JZ4886 (Cysteine) 0 (GPI-Linked Proteins) 0 (RECK protein, human) |
تواريخ الأحداث: | Date Created: 20221230 Date Completed: 20230103 Latest Revision: 20230103 |
رمز التحديث: | 20240628 |
PMID: | 36585230 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1007-8738 |
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