دورية أكاديمية
Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment.
| العنوان: | Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment. |
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| المؤلفون: | Kachale A; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.; Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic., Pavlíková Z; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Nenarokova A; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.; Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic.; School of Biological Sciences, University of Bristol, Bristol, UK., Roithová A; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Durante IM; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic., Miletínová P; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Záhonová K; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.; Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic.; Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic., Nenarokov S; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.; Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic., Votýpka J; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.; Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic., Horáková E; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.; Institute of Microbiology, Czech Academy of Sciences, Třeboň, Czech Republic., Ross RL; Thermo Fisher Scientific, Franklin, MA, USA., Yurchenko V; Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic., Beznosková P; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Paris Z; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic. parda@paru.cas.cz.; Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic. parda@paru.cas.cz., Valášek LS; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic. valasekl@biomed.cas.cz., Lukeš J; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic. jula@paru.cas.cz.; Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic. jula@paru.cas.cz. |
| المصدر: | Nature [Nature] 2023 Jan; Vol. 613 (7945), pp. 751-758. Date of Electronic Publication: 2023 Jan 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basingstoke : Nature Publishing Group Original Publication: London, Macmillan Journals ltd. |
| مواضيع طبية MeSH: | Anticodon*/chemistry , Anticodon*/genetics , Anticodon*/metabolism , Codon, Terminator*/genetics , Genetic Code*/genetics , Mutation* , Peptide Termination Factors*/genetics , Peptide Termination Factors*/metabolism , RNA, Transfer*/genetics , RNA, Transfer*/metabolism , Eukaryotic Cells*, Ciliophora/genetics ; RNA, Transfer, Trp/genetics ; Saccharomyces cerevisiae/genetics ; RNA, Transfer, Glu/genetics ; Trypanosoma brucei brucei/genetics |
| مستخلص: | Cognate tRNAs deliver specific amino acids to translating ribosomes according to the standard genetic code, and three codons with no cognate tRNAs serve as stop codons. Some protists have reassigned all stop codons as sense codons, neglecting this fundamental principle 1-4 . Here we analyse the in-frame stop codons in 7,259 predicted protein-coding genes of a previously undescribed trypanosomatid, Blastocrithidia nonstop. We reveal that in this species in-frame stop codons are underrepresented in genes expressed at high levels and that UAA serves as the only termination codon. Whereas new tRNAs Glu fully cognate to UAG and UAA evolved to reassign these stop codons, the UGA reassignment followed a different path through shortening the anticodon stem of tRNA Trp (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.) |
| التعليقات: | Comment in: Nature. 2023 Jan;613(7945):631-632. (PMID: 36631582) Erratum in: Nature. 2023 Jun;618(7965):E23. (PMID: 37258684) Erratum in: Nature. 2024 Jan 18;:. (PMID: 38238539) |
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| المشرفين على المادة: | 0 (Anticodon) 0 (Codon, Terminator) 0 (Peptide Termination Factors) 9014-25-9 (RNA, Transfer) 0 (RNA, Transfer, Trp) 0 (RNA, Transfer, Glu) |
| تواريخ الأحداث: | Date Created: 20230111 Date Completed: 20230201 Latest Revision: 20240118 |
| رمز التحديث: | 20240119 |
| DOI: | 10.1038/s41586-022-05584-2 |
| PMID: | 36631608 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1476-4687 |
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| DOI: | 10.1038/s41586-022-05584-2 |