دورية أكاديمية
أعرض في EDS

Using SuperClomeleon to Measure Changes in Intracellular Chloride during Development and after Early Life Stress.

التفاصيل البيبلوغرافية
العنوان: Using SuperClomeleon to Measure Changes in Intracellular Chloride during Development and after Early Life Stress.
المؤلفون: Herstel LJ; Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands., Peerboom C; Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands., Uijtewaal S; Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands., Selemangel D; Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands., Karst H; Utrecht Brain Center, University Medical Center Utrecht, 3584 CX, Utrecht, The Netherlands., Wierenga CJ; Cell Biology, Neurobiology and Biophysics, Biology Department, Utrecht University, 3584 CH, Utrecht, The Netherlands corette.wierenga@donders.ru.nl.
المصدر: ENeuro [eNeuro] 2022 Dec 23; Vol. 9 (6). Date of Electronic Publication: 2022 Dec 23 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 101647362 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 2373-2822 (Electronic) Linking ISSN: 23732822 NLM ISO Abbreviation: eNeuro Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Washington, DC] : Society for Neuroscience, [2014]-
مواضيع طبية MeSH: Chlorides*/metabolism , Stress, Physiological*, Animals ; Female ; Male ; Mice ; gamma-Aminobutyric Acid/pharmacology ; Neurons/physiology ; Receptors, GABA-A
مستخلص: Intraneuronal chloride concentrations ([Cl - ] i ) decrease during development resulting in a shift from depolarizing to hyperpolarizing GABA responses via chloride-permeable GABA A receptors. This GABA shift plays a pivotal role in postnatal brain development, and can be strongly influenced by early life experience. Here, we assessed the applicability of the recently developed fluorescent SuperClomeleon (SClm) sensor to examine changes in [Cl - ] i using two-photon microscopy in brain slices. We used SClm mice of both sexes to monitor the developmental decrease in neuronal chloride levels in organotypic hippocampal cultures. We could discern a clear reduction in [Cl - ] i between day in vitro (DIV)3 and DIV9 (equivalent to the second postnatal week in vivo ) and a further decrease in some cells until DIV22. In addition, we assessed alterations in [Cl - ] i in the medial prefrontal cortex (mPFC) of postnatal day (P)9 male SClm mouse pups after early life stress (ELS). ELS was induced by limiting nesting material between P2 and P9. ELS induced a shift toward higher (i.e., immature) chloride levels in layer 2/3 cells in the mPFC. Although conversion from SClm fluorescence to absolute chloride concentrations proved difficult, our study underscores that the SClm sensor is a powerful tool to measure physiological changes in [Cl - ] i in brain slices.
Competing Interests: The authors declare no competing financial interests.
(Copyright © 2022 Herstel et al.)
References: Annu Rev Neurosci. 2004;27:549-79. (PMID: 15217343)
Nat Commun. 2015 Jul 16;6:7750. (PMID: 26177896)
Genesis. 2001 Sep;31(1):37-42. (PMID: 11668676)
J Comp Neurol. 2004 Jan 1;468(1):57-64. (PMID: 14648690)
Sci Rep. 2017 Sep 19;7(1):11878. (PMID: 28928398)
Dev Med Child Neurol. 2019 Oct;61(10):1127-1133. (PMID: 30740660)
J Neuroinflammation. 2022 Jun 15;19(1):151. (PMID: 35705972)
J Neurosci. 2008 Feb 13;28(7):1557-67. (PMID: 18272677)
J Physiol. 1987 Apr;385:243-86. (PMID: 2443667)
Acta Physiol (Oxf). 2018 Jun;223(2):e13066. (PMID: 29575542)
Neuron. 2009 Sep 10;63(5):657-72. (PMID: 19755108)
Epilepsia. 2007;48 Suppl 5:96-105. (PMID: 17910587)
Physiol Rev. 2007 Oct;87(4):1215-84. (PMID: 17928584)
Front Cell Neurosci. 2014 Aug 29;8:258. (PMID: 25221475)
Neurobiol Stress. 2015 Dec 14;3:1-7. (PMID: 26844244)
Biochem Biophys Res Commun. 2017 Nov 25;493(3):1243-1249. (PMID: 28962859)
J Neurosci. 2021 Jun 9;41(23):4957-4975. (PMID: 33903223)
Eur J Neurosci. 2022 Nov;56(10):5763-5783. (PMID: 36117291)
J Physiol. 1999 Sep 15;519 Pt 3:693-712. (PMID: 10457084)
Anal Biochem. 1989 May 1;178(2):355-61. (PMID: 2751097)
Neuron. 2013 Oct 2;80(1):198-209. (PMID: 24012279)
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3245-50. (PMID: 9501248)
J Neurosci Methods. 1991 Apr;37(2):173-82. (PMID: 1715499)
Nat Rev Neurosci. 2014 Oct;15(10):637-54. (PMID: 25234263)
Endocrinology. 2008 Oct;149(10):4892-900. (PMID: 18566122)
J Neurochem. 2022 Mar 14;:. (PMID: 35285522)
Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8770-E8779. (PMID: 28973889)
J Neurosci. 2012 Mar 21;32(12):4017-31. (PMID: 22442068)
Neuropharmacology. 2020 Dec 1;180:108294. (PMID: 32882227)
eNeuro. 2021 Mar 9;8(2):. (PMID: 33239270)
Brain Cell Biol. 2006 Dec;35(4-6):207-28. (PMID: 18398684)
J Neuroendocrinol. 2015 Jun;27(6):446-56. (PMID: 25737097)
Biochemistry. 1987 Mar 10;26(5):1215-9. (PMID: 3567167)
J Physiol. 2004 Jun 15;557(Pt 3):829-41. (PMID: 15090604)
Eur J Neurosci. 2015 Feb;41(4):442-53. (PMID: 25581710)
Front Cell Neurosci. 2018 Jan 25;12:7. (PMID: 29422838)
Sci Adv. 2020 Jun 12;6(24):eaba1430. (PMID: 32582852)
Hippocampus. 2015 Mar;25(3):309-28. (PMID: 25269685)
Nat Rev Neurosci. 2016 Sep 19;17(10):652-66. (PMID: 27640984)
J Neurosci Methods. 2022 Feb 15;368:109455. (PMID: 34952088)
Neuroscience. 2018 Aug 21;386:91-107. (PMID: 29949744)
Neuron. 2000 Sep;27(3):447-59. (PMID: 11055428)
J Physiol. 2014 Jan 1;592(1):13-31. (PMID: 24144877)
J Neurophysiol. 2008 Mar;99(3):1523-34. (PMID: 18216229)
Front Cell Neurosci. 2010 Apr 14;4:11. (PMID: 20428495)
Nature. 1999 Jan 21;397(6716):251-5. (PMID: 9930699)
Neurosci Biobehav Rev. 2021 May;124:179-192. (PMID: 33549742)
Annu Rev Pharmacol Toxicol. 1982;22:465-90. (PMID: 7044290)
J Neurosci. 2013 Oct 9;33(41):16297-309. (PMID: 24107961)
Annu Rev Neurosci. 2022 Jul 8;45:273-294. (PMID: 35316611)
Cell. 1996 Dec 27;87(7):1317-26. (PMID: 8980237)
Prog Neurobiol. 1994 Mar;42(4):489-537. (PMID: 7522334)
J Cell Sci. 2020 Jan 23;133(2):. (PMID: 31974277)
J Physiol. 1993 May;464:273-89. (PMID: 8229801)
Nat Methods. 2010 Jul;7(7):516-8. (PMID: 20581829)
Psychoneuroendocrinology. 2016 Aug;70:85-97. (PMID: 27179233)
Front Behav Neurosci. 2018 Jul 31;12:157. (PMID: 30108490)
فهرسة مساهمة: Keywords: FRET sensor; GABA shift; chloride; two-photon microscopy
المشرفين على المادة: 0 (Chlorides)
56-12-2 (gamma-Aminobutyric Acid)
0 (Receptors, GABA-A)
تواريخ الأحداث: Date Created: 20230112 Date Completed: 20230118 Latest Revision: 20230201
رمز التحديث: 20230201
مُعرف محوري في PubMed: PMC9797207
DOI: 10.1523/ENEURO.0416-22.2022
PMID: 36635254
قاعدة البيانات: MEDLINE
الوصف
تدمد:2373-2822
DOI:10.1523/ENEURO.0416-22.2022