دورية أكاديمية
Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy.
| العنوان: | Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy. |
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| المؤلفون: | Zink A; Institute of Immunodeficiency, Medical Center and Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany. Electronic address: alicia.zink@uniklinik-freiburg.de., Zenke S; Institute of Immunodeficiency, Medical Center and Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany., Wiese T; Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany., Beyersdorf N; Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany., Lämmermann T; Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany., Rohr JC; Institute of Immunodeficiency, Medical Center and Faculty of Medicine, Albert-Ludwigs-University, Freiburg, Germany. Electronic address: jan.rohr@uniklinik-freiburg.de. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 102013. Date of Electronic Publication: 2023 Jan 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | T-Lymphocytes* , Trogocytosis*, Flow Cytometry ; Microscopy, Confocal ; Coculture Techniques |
| مستخلص: | Here, we present a protocol to examine the mechanisms underlying the intercellular transfer of transmembrane molecules, termed trogocytosis, and the fate of transferred molecules. We describe the steps needed from T lymphocyte isolation, via co-culture with cells expressing the ligand of interest, to cell harvest and subsequent staining for flow cytometry and confocal microscopy. Furthermore, we showcase critical parameters and pitfalls, which allow easy adaptation of the protocol to investigate trogocytosis of various cell surface receptors in different cell types. For complete details on the use and execution of this protocol, please refer to Zink and Rohr. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell Biology; Cell Membrane; Cell-based Assays; Flow Cytometry/Mass Cytometry; Immunology |
| تواريخ الأحداث: | Date Created: 20230113 Date Completed: 20231023 Latest Revision: 20231024 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC9852653 |
| DOI: | 10.1016/j.xpro.2022.102013 |
| PMID: | 36638014 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2022.102013 |