دورية أكاديمية
Short-chain fatty acid production in accessible and inaccessible body pools as assessed by novel stable tracer pulse approach is reduced by aging independent of presence of COPD.
| العنوان: | Short-chain fatty acid production in accessible and inaccessible body pools as assessed by novel stable tracer pulse approach is reduced by aging independent of presence of COPD. |
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| المؤلفون: | Kirschner SK; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA., Ghane P; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA; Department of Electrical & Computer Engineering, Texas A&M University, College Station, TX, USA., Park JK; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA., Simbo SY; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA., Ivanov I; Department of Electrical & Computer Engineering, Texas A&M University, College Station, TX, USA; Department of Veterinary Physiology & Pharmacology, Texas A&M University, College Station, TX, USA., Braga-Neto UM; Department of Electrical & Computer Engineering, Texas A&M University, College Station, TX, USA., Ten Have GAM; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA., Thaden JJ; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA., Engelen MPKJ; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA., Deutz NEP; Center for Translational Research in Aging & Longevity, Texas A&M University, College Station, TX, USA. Electronic address: nep.deutz@ctral.org. |
| المصدر: | Metabolism: clinical and experimental [Metabolism] 2023 Apr; Vol. 141, pp. 155399. Date of Electronic Publication: 2023 Jan 13. |
| نوع المنشور: | Observational Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 0375267 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-8600 (Electronic) Linking ISSN: 00260495 NLM ISO Abbreviation: Metabolism Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Elsevier Inc. Original Publication: New York, Grune & Stratton. |
| مواضيع طبية MeSH: | Propionates* , Fatty Acids, Volatile*, Young Adult ; Humans ; Aged ; Acetates/metabolism ; Butyrates ; Aging |
| مستخلص: | Background: Production rates of the short-chain fatty acids (SCFA) acetate, propionate, and butyrate, which are beneficial metabolites of the intestinal microbiota, are difficult to measure in humans due to inaccessibility of the intestine to perform measurements, and the high first-pass metabolism of SCFAs in colonocytes and liver. We developed a stable tracer pulse approach to estimate SCFA whole-body production (WBP) in the accessible pool representing the systemic circulation and interstitial fluid. Compartmental modeling of plasma enrichment data allowed us to additionally calculate SCFA kinetics and pool sizes in the inaccessible pool likely representing the intestine with microbiota. We also studied the effects of aging and the presence of Chronic Obstructive Pulmonary Disease (COPD) on SCFA kinetics. Methods: In this observational study, we designed a two-compartmental model to determine SCFA kinetics in 31 young (20-29 y) and 71 older (55-87 y) adults, as well as in 33 clinically stable patients with moderate to very severe COPD (mean (SD) FEV Results: Acetate, propionate, and butyrate WBP and fluxes between the accessible and inaccessible pools were lower in older than young adults (all q < 0.0001). Moreover, older participants had lower acetate (q < 0.0001) and propionate (q = 0.019) production rates in the inaccessible pool as well as smaller sizes of the accessible and inaccessible acetate pools (both q < 0.0001) than young participants. WBP, compartmental SCFA kinetics, and pool sizes did not differ between COPD patients and older adults (all q > 0.05). Overall and independent of the group studied, calculated production rates in the inaccessible pool were on average 7 (acetate), 11 (propionate), and 16 (butyrate) times higher than non-compartmental WBP, and sizes of inaccessible pools were 24 (acetate), 31 (propionate), and 55 (butyrate) times higher than sizes of accessible pools (all p < 0.0001). Conclusion: Non-compartmental production measurements of SCFAs in the accessible pool (i.e. systemic circulation) substantially underestimate the SCFA production in the inaccessible pool, which likely represents the intestine with microbiota, as assessed by compartmental analysis. Competing Interests: Declaration of competing interest The authors have nothing to disclose. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aging; Chronic Obstructive Pulmonary Disease; Compartmental modeling; SCFA production rate; SCFA rate of appearance; Stable isotopes |
| المشرفين على المادة: | 0 (Propionates) 0 (Fatty Acids, Volatile) 0 (Acetates) 0 (Butyrates) |
| تواريخ الأحداث: | Date Created: 20230115 Date Completed: 20230228 Latest Revision: 20230315 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.metabol.2023.155399 |
| PMID: | 36642114 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-8600 |
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| DOI: | 10.1016/j.metabol.2023.155399 |