دورية أكاديمية
Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection.
| العنوان: | Pathways Activated by Infected and Bystander Chondrocytes in Response to Ross River Virus Infection. |
|---|---|
| المؤلفون: | Lim EXY; Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia., Webster JA; Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia., Rudd PA; Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia., Herrero LJ; Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia. |
| المصدر: | Viruses [Viruses] 2022 Dec 31; Vol. 15 (1). Date of Electronic Publication: 2022 Dec 31. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI |
| مواضيع طبية MeSH: | Alphavirus*/genetics , Alphavirus Infections*, Humans ; Chondrocytes/metabolism ; Ross River virus/genetics ; Ross River virus/metabolism |
| مستخلص: | Old world alphaviruses, such as Ross River virus (RRV), cause debilitating arthralgia during acute and chronic stages of the disease. RRV-induced cartilage degradation has been implicated as a cause of joint pain felt by RRV patients. Chondrocytes are a major cell type of cartilage and are involved in the production and maintenance of the cartilage matrix. It is thought that these cells may play a vital role in RRV disease pathogenesis. In this study, we used RNA-sequencing (RNA-Seq) to examine the transcriptomes of RRV-infected and bystander chondrocytes in the same environment. RRV containing green fluorescent protein (GFP) allowed for the separation of RRV-infected (GFP+) and bystander uninfected cells (GFP-). We found that whereas GFP+ and GFP- populations commonly presented similar gene expression profiles during infection, there were also unique signatures. For example, RIMS2 and FOXJ1 were unique to GFP+ cells, whilst Aim2 and CCL8 were only found in bystander chondrocytes. This indicates that careful selection of potential therapeutic targets is important to minimise adverse effects to the neighbouring uninfected cell populations. Our study serves as a resource to provide more information about the pathways and responses elicited by RRV in cells which are both infected and stimulated because of neighbouring infected cells. |
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| فهرسة مساهمة: | Keywords: alphavirus; arthritis; cartilage; extracellular matrix |
| تواريخ الأحداث: | Date Created: 20230121 Date Completed: 20230124 Latest Revision: 20231116 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9864161 |
| DOI: | 10.3390/v15010136 |
| PMID: | 36680176 |
| قاعدة البيانات: | MEDLINE |
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