دورية أكاديمية
Separation of Isomeric Forms of Urolithin Glucuronides Using Supercritical Fluid Chromatography.
| العنوان: | Separation of Isomeric Forms of Urolithin Glucuronides Using Supercritical Fluid Chromatography. |
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| المؤلفون: | Ares AM; I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Faculty of Sciences, University of Valladolid, Valladolid 47011, Spain., Toribio L; I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Faculty of Sciences, University of Valladolid, Valladolid 47011, Spain., García-Villalba R; CEBAS-CSIC, Research Group on Quality, Safety, and Bioactivity of Plant-Derived Foods, P.O. Box 164, Espinardo, Murcia 30100, Spain., Villalgordo JM; Eurofins-VillaPharma Research S.L.; Parque Tecnológico de Fuente Álamo, Fuente Álamo, Murcia E-30320, Spain., Althobaiti Y; Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Tomás-Barberán FA; CEBAS-CSIC, Research Group on Quality, Safety, and Bioactivity of Plant-Derived Foods, P.O. Box 164, Espinardo, Murcia 30100, Spain.; Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Bernal J; I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Faculty of Sciences, University of Valladolid, Valladolid 47011, Spain. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2023 Feb 15; Vol. 71 (6), pp. 3033-3039. Date of Electronic Publication: 2023 Jan 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Chromatography, Supercritical Fluid* , Gastrointestinal Microbiome*, Humans ; Glucuronides/metabolism ; Coumarins/chemistry ; Hydrolyzable Tannins/metabolism |
| مستخلص: | Urolithins are gut microbiota metabolites produced in humans after consuming foods containing ellagitannins and ellagic acid. Three urolithin metabotypes have been reported for different individuals depending on the final urolithins produced. After absorption, they are conjugated with glucuronic acid (phase II metabolism), and these are the main circulating metabolites in plasma and reach different tissues. Different regioisomeric isomers of urolithin glucuronides have been described. Still, their identification and quantification in humans have not been properly reported due to resolution limitations in their analysis by reversed-phase high-performance liquid chromatography. In the present study, we report a novel method for separating these isomers using supercritical fluid chromatography. With this method, urolithin A 3- and 8-glucuronide, isourolithin A 3- and 9- glucuronide, and urolithin B 3-glucuronide (8-hydroxy urolithin 3-glucuronide; 3-hydroxy urolithin 8-glucuronide; 3-hydroxyurolithin 9-glucuronide; 9-hydroxyurolithin 3-glucuronide; and urolithin 3-glucuronide) were separated in less than 15 min. The proposed method was applied to successfully analyze these metabolites in urine samples from different volunteers belonging to different metabotypes. |
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| فهرسة مساهمة: | Keywords: chromatographic separation; glucuronides; gut microbiota metabolites; supercritical fluid chromatography; urolithins |
| المشرفين على المادة: | 0 (Glucuronides) 0 (Coumarins) 0 (Hydrolyzable Tannins) |
| تواريخ الأحداث: | Date Created: 20230131 Date Completed: 20230216 Latest Revision: 20230220 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC9936581 |
| DOI: | 10.1021/acs.jafc.2c07145 |
| PMID: | 36719954 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.2c07145 |