دورية أكاديمية
Outbreak of Chagas disease in Brazil: Validation of a molecular diagnostic method.
| العنوان: | Outbreak of Chagas disease in Brazil: Validation of a molecular diagnostic method. |
|---|---|
| المؤلفون: | Costa-Oliveira CND; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Paiva-Cavalcanti M; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil. Electronic address: milena.cavalcanti@fiocruz.br., Barros MDS; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Nakazawa M; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Melo MGN; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Pessoa-E-Silva R; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Torres DJL; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Oliveira KKDS; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Moreira LR; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Morais RCS; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Goes TC; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil., Oliveira GMA; Health Secretariat of Pernambuco, Recife, Pernambuco, Brazil., Júnior WO; Cardiac Emergency of Pernambuco (PROCAPE) - Pernambuco University (UPE), Recife, Pernambuco, Brazil., Silva MMME; University Hospital Oswaldo Cruz (HUOC) - Pernambuco University (UPE), Recife, Pernambuco, Brazil., Batista FP; University Hospital Oswaldo Cruz (HUOC) - Pernambuco University (UPE), Recife, Pernambuco, Brazil., Montenegro D; University Hospital Oswaldo Cruz (HUOC) - Pernambuco University (UPE), Recife, Pernambuco, Brazil., Lorena VMB; Aggeu Magalhães Institute, Recife, Pernambuco, Brazil. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2023 Apr; Vol. 247, pp. 108478. Date of Electronic Publication: 2023 Jan 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Chagas Disease*/diagnosis , Chagas Disease*/epidemiology , Chagas Disease*/drug therapy , Trypanosoma cruzi*/genetics, Humans ; Brazil/epidemiology ; Pathology, Molecular ; DNA, Protozoan/genetics ; Real-Time Polymerase Chain Reaction/methods ; Disease Outbreaks |
| مستخلص: | Chagas disease (CD), caused by the protozoan Trypanosoma cruzi (T. cruzi), affects millions of people worldwide. Polymerase Chain Reaction (PCR) and real-time quantitative PCR (qPCR) have been used as tools to monitor parasitic levels in the bloodstream of individuals exposed to infection, thus enabling the monitoring of relapses and the effectiveness of therapy, for example. The aim of this study was to evaluate the TcSAT-IAM system, developed by our research group, on samples from patients with suspected Chagas disease infection. Initially, primer systems were developed for the detection of the nuclear DNA (SAT-DNA) from T. cruzi (TcSAT-IAM). The Cruzi system, predicted in the literature, and TcSAT-IAM were then evaluated in relation to their analytical sensitivity, specificity and efficiency. Afterwards, the applicability of the qPCR technique using both systems (separately) for the diagnosis of acute CD was evaluated in samples from 77 individuals exposed to the outbreak that occurred in Pernambuco-Brazil, relating the results obtained to those of the classical diagnostic methods recommended for this stage of the infection. TcSAT-IAM and Cruzi had a detection limit of 1 fg of target DNA (0,003 parasites). Thirty-eight cases were recorded, 28 by laboratory criteria and 10 by clinical and epidemiological criteria. Blood samples from 77 subjects were submitted to qPCR by both systems, reaching an agreement of 89.61% between them. After analyzes between systems and diagnostic criteria, the TcSAT-IAM showed sensitivity and specificity of 52.36% (CI 37.26-67.52) and 92.31% (CI 79.68-97.35), respectively, accuracy of 72.73% and moderate agreement. The TcSAT-IAM showed an accuracy of 72.58% and 75% in relation to parasitological and serological tests (IgM anti-T. cruzi), respectively. Therefore, quantitative PCR should be incorporated into the diagnosis of suspected acute cases of Chagas disease. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Acute phase; Chagas disease; Diagnosis; Real-time PCR |
| المشرفين على المادة: | 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 20230202 Date Completed: 20230320 Latest Revision: 20230320 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.exppara.2023.108478 |
| PMID: | 36731642 |
| قاعدة البيانات: | MEDLINE |
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