دورية أكاديمية
A novel high-prevalence antigen in the Lutheran system, LUGA (LU24), and an updated, full-length 3D BCAM model.
| العنوان: | A novel high-prevalence antigen in the Lutheran system, LUGA (LU24), and an updated, full-length 3D BCAM model. |
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| المؤلفون: | Floch A; Univ Paris Est Creteil, INSERM U955 Equipe Transfusion et maladies du globule rouge, IMRB, Creteil, France.; Laboratoire de Biologie Médicale de Référence en Immuno-hématologie moléculaire, Etablissement francais du sang Ile-de-France, Creteil, France.; Immunohematology and Genomics Laboratory, New York Blood Center, New York, New York, USA., Lomas-Francis C; Immunohematology and Genomics Laboratory, New York Blood Center, New York, New York, USA., Vege S; Immunohematology and Genomics Laboratory, New York Blood Center, New York, New York, USA., Brennan S; Red Cell Reference Laboratory, American Red Cross-Northwest Region, Portland, Oregon, USA., Shakarian G; Immunohematology and Genomics Laboratory, New York Blood Center, New York, New York, USA., de Brevern AG; Université Paris Cité, Biologie Intégrée du Globule Rouge UMR_S1134, Inserm, Université de la Réunion, Université des Antilles, Paris, France., Westhoff CM; Immunohematology and Genomics Laboratory, New York Blood Center, New York, New York, USA. |
| المصدر: | Transfusion [Transfusion] 2023 Apr; Vol. 63 (4), pp. 798-807. Date of Electronic Publication: 2023 Feb 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association Of Blood Banks Country of Publication: United States NLM ID: 0417360 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-2995 (Electronic) Linking ISSN: 00411132 NLM ISO Abbreviation: Transfusion Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Arlington, Va. : American Association Of Blood Banks |
| مواضيع طبية MeSH: | Cell Adhesion Molecules*/genetics , Protestantism*, Humans ; Prevalence ; Erythrocytes/metabolism ; Lutheran Blood-Group System/genetics |
| مستخلص: | Background: The basal cell adhesion molecule (BCAM) carries the antigens of the Lutheran (LU, ISBT005) system. We report a novel Lutheran antigen and propose an updated, full-length 3D model of BCAM. Study Design and Methods: Red blood cell testing, antibody identification, and BCAM genomic DNA sequencing were done by standard methods. Multi-template homology modeling of BCAM used structural templates selected for coverage, highest sequence identity, and protein domain family. All variants causing the loss or gain of a Lutheran antigen were analyzed for residue accessibility and intraprotein interactions. Results: An antibody to a high-prevalence antigen in the plasma of a pregnant woman was determined to be directed at a novel Lutheran antigen. Sequencing of BCAM found three homozygous changes: c.212G > A (p.Arg71His) and two silent, c.711C > T and c.714C > T. The model was built from the first two immunoglobulin crystallized domains of BCAM (D1, D2), three other templates (for D3, D4 and D5 with a higher sequence identity with the target than those used for the model proposed by Burton and Brady in 2008, and for the transmembrane region) and RaptorX (for the intracellular domain). All residues associated with a Lutheran antigen were found to be exposed in wild-type or variant proteins, except p.447 associated with loss of Lu13 expression. Conclusion: The c.212G > A change results in the loss of LUGA (LU24) antigen. Whole genome sequencing continues to reveal polymorphisms with uncertain immunogenicity. This model and demonstration that nearly all residues associated with the expression of a Lutheran antigen are exposed will help evaluate the significance of new polymorphisms. (© 2023 AABB.) |
| فهرسة مساهمة: | Keywords: BCAM; LU; Lutheran; antigen; blood group; genomics; molecular modeling; red blood cell |
| المشرفين على المادة: | 0 (Cell Adhesion Molecules) 0 (Lutheran Blood-Group System) 0 (BCAM protein, human) |
| تواريخ الأحداث: | Date Created: 20230204 Date Completed: 20230412 Latest Revision: 20230624 |
| رمز التحديث: | 20230625 |
| DOI: | 10.1111/trf.17262 |
| PMID: | 36738255 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1537-2995 |
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| DOI: | 10.1111/trf.17262 |