Scanless two-photon voltage imaging.
| العنوان: | Scanless two-photon voltage imaging. |
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| المؤلفون: | Sims RR; Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France., Bendifallah I; Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France., Grimm C; Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France., Mohamed-Lafirdeen A; Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France., Lu X; Systems, Synthetic, and Physical Biology Program, Rice University, Houston, TX, USA., St-Pierre F; Systems, Synthetic, and Physical Biology Program, Rice University, Houston, TX, USA.; Department of Neuroscience and Department of Biochemistry and Molecular Biology, Houston, TX, USA.; Department of Electrical and Computer Engineering, Rice University, Houston, TX, USA., Papagiakoumou E; Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France., Emiliani V; Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France. |
| المصدر: | Research square [Res Sq] 2023 Jan 24. Date of Electronic Publication: 2023 Jan 24. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101768035 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: Res Sq Subsets: PubMed not MEDLINE |
| مستخلص: | Parallel light-sculpting methods have been used to perform scanless two-photon photostimulation of multiple neurons simultaneously during all-optical neurophysiology experiments. We demonstrate that scanless two-photon excitation also enables high-resolution, high-contrast, voltage imaging by efficiently exciting fluorescence in a large fraction of the cellular soma. We present a thorough characterisation of scanless two-photon voltage imaging using existing parallel approaches and lasers with different repetition rates. We demonstrate voltage recordings of high frequency spike trains and sub-threshold depolarizations in intact brain tissue from neurons expressing the soma-targeted genetically encoded voltage indicator JEDI-2P-kv. Using a low repetition-rate laser, we perform recordings from up to ten neurons simultaneously. Finally, by co-expressing JEDI-2P-kv and the channelrhodopsin ChroME-ST in neurons of hippocampal organotypic slices, we perform single-beam, simultaneous, two-photon voltage imaging and photostimulation. This enables in-situ validation of the precise number and timing of light evoked action potentials and will pave the way for rapid and scalable identification of functional brain connections in intact neural circuits. |
| التعليقات: | Update in: Nat Commun. 2024 Jun 14;15(1):5095. doi: 10.1038/s41467-024-49192-2. (PMID: 38876987) |
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| معلومات مُعتمدة: | R01 EB032854 United States EB NIBIB NIH HHS; U01 NS118288 United States NS NINDS NIH HHS; P50 HD103555 United States HD NICHD NIH HHS; U01 NS113294 United States NS NINDS NIH HHS; R01 EB027145 United States EB NIBIB NIH HHS |
| فهرسة مساهمة: | Keywords: Two-photon microscopy; computer-generated holography; optogenetics; temporal focusing; voltage imaging |
| تواريخ الأحداث: | Date Created: 20230207 Latest Revision: 20240624 |
| رمز التحديث: | 20240624 |
| مُعرف محوري في PubMed: | PMC9900978 |
| DOI: | 10.21203/rs.3.rs-2412371/v1 |
| PMID: | 36747617 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.21203/rs.3.rs-2412371/v1 |
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