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Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX.

التفاصيل البيبلوغرافية
العنوان: Small extracellular vesicles released from germinated kiwi pollen (pollensomes) present characteristics similar to mammalian exosomes and carry a plant homolog of ALIX.
المؤلفون: Suanno C; University of Bologna, Department of Biological, Geological, and Environmental Sciences, Bologna, Italy., Tonoli E; Nottingham Trent University, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham, United Kingdom., Fornari E; Chrysalis Health & Beauty Creation House, Nottingham, United Kingdom., Savoca MP; Nottingham Trent University, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham, United Kingdom., Aloisi I; University of Bologna, Department of Biological, Geological, and Environmental Sciences, Bologna, Italy., Parrotta L; University of Bologna, Department of Biological, Geological, and Environmental Sciences, Bologna, Italy.; University of Bologna, Interdepartmental Centre for Agri-Food Industrial Research, Cesena, Italy., Faleri C; University of Siena, Department of Life Sciences, Siena, Italy., Cai G; University of Siena, Department of Life Sciences, Siena, Italy., Coveney C; Nottingham Trent University, Department of Biosciences, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham, United Kingdom.; Nottingham Trent University, John van Geest Cancer Research Centre, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham, United Kingdom., Boocock DJ; Nottingham Trent University, Department of Biosciences, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham, United Kingdom.; Nottingham Trent University, John van Geest Cancer Research Centre, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham, United Kingdom., Verderio EAM; University of Bologna, Department of Biological, Geological, and Environmental Sciences, Bologna, Italy.; Nottingham Trent University, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham, United Kingdom., Del Duca S; University of Bologna, Department of Biological, Geological, and Environmental Sciences, Bologna, Italy.; University of Bologna, Interdepartmental Centre for Agri-Food Industrial Research, Cesena, Italy.
المصدر: Frontiers in plant science [Front Plant Sci] 2023 Jan 25; Vol. 14, pp. 1090026. Date of Electronic Publication: 2023 Jan 25 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101568200 Publication Model: eCollection Cited Medium: Print ISSN: 1664-462X (Print) Linking ISSN: 1664462X NLM ISO Abbreviation: Front Plant Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation, 2010-
مستخلص: Introduction: In the last decade, it has been discovered that allergen-bearing extracellular nanovesicles, termed "pollensomes", are released by pollen during germination. These extracellular vesicles (EVs) may play an important role in pollen-pistil interaction during fertilization, stabilizing the secreted bioactive molecules and allowing long-distance signaling. However, the molecular composition and the biological role of these EVs are still unclear. The present study had two main aims: (I) to clarify whether pollen germination is needed to release pollensomes, or if they can be secreted also in high humidity conditions; and (II) to investigate the molecular features of pollensomes following the most recent guidelines for EVs isolation and identification.
Methods: To do so, pollensomes were isolated from hydrated and germinated kiwi ( Actinidia chinensis Planch.) pollen, and characterized using imaging techniques, immunoblotting, and proteomics.
Results: These analyses revealed that only germinated kiwi pollen released detectable concentrations of nanoparticles compatible with small EVs for shape and protein content. Moreover, a plant homolog of ALIX, which is a well-recognized and accepted marker of small EVs and exosomes in mammals, was found in pollensomes.
Discussion: The presence of this protein, along with other proteins involved in endocytosis, is consistent with the hypothesis that pollensomes could comprehend a prominent subpopulation of plant exosome-like vesicles.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Suanno, Tonoli, Fornari, Savoca, Aloisi, Parrotta, Faleri, Cai, Coveney, Boocock, Verderio and Del Duca.)
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فهرسة مساهمة: Keywords: ALIX; EVs; exosomes; nanovesicles; pollen tube; pollensomes; pollination; signaling
تواريخ الأحداث: Date Created: 20230210 Latest Revision: 20240912
رمز التحديث: 20240912
مُعرف محوري في PubMed: PMC9905850
DOI: 10.3389/fpls.2023.1090026
PMID: 36760648
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-462X
DOI:10.3389/fpls.2023.1090026