دورية أكاديمية
أعرض في EDS

Melatonin Mitigates iNOS-Related Effects of HEMA and Camphorquinone in Human Dental Pulp Cells: Relevance for Postoperative Sensitivity Mechanism in Type 2 Diabetes.

التفاصيل البيبلوغرافية
العنوان: Melatonin Mitigates iNOS-Related Effects of HEMA and Camphorquinone in Human Dental Pulp Cells: Relevance for Postoperative Sensitivity Mechanism in Type 2 Diabetes.
المؤلفون: Ilić J; Department of Restorative Odontology and Endodontics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia., Milosavljević A; Department of Pharmacology in Dentistry, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia., Lazarević M; Department of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia., Milošević Marković M; Department of Public Health, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia., Milašin J; Department of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia., Vučetić M; Department of Oral Surgery, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia., Chaurasia A; Department of Oral Medicine and Radiology, Faculty of Dental Sciences, King George's Medical University, Lucknow 226003, India., Miletić V; Sydney Dental School, Faculty of Medicine and Health, University of Sydney, Sydney 2145, Australia., Roganović J; Department of Pharmacology in Dentistry, School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Jan 29; Vol. 24 (3). Date of Electronic Publication: 2023 Jan 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Melatonin*/pharmacology , Diabetes Mellitus, Type 2*/drug therapy, Humans ; Methacrylates/pharmacology ; Nitric Oxide Synthase Type II/genetics ; Nitric Oxide Synthase Type II/metabolism ; Dental Pulp/metabolism ; Antioxidants ; Proto-Oncogene Proteins c-bcl-2/metabolism
مستخلص: High elution and diffusion of 2-hydroxylethyl methacrylate (HEMA) and camphorquinone (CQ) through dentinal tubules may induce pulp injury and postoperative sensitivity. We aimed to investigate the melatonin protective effect in HEMA- and CQ-treated human dental pulp cells (hDPCs) as well as its relevance in a mechanism for postoperative sensitivity in diabetic patients. hDPCs were exposed to HEMA (5 mM) and/or CQ (1 mM) in the absence and presence of melatonin (MEL) (0.1 mM and 1 mM). Heme oxygenase-1 (HMOX1), NADPH oxidase-4 (NOX4), BCL-2-associated X-protein (BAX), B-cell lymphoma-2 (BCL-2) and caspase-3 (CASP3) gene expression levels, and superoxide dismutase (SOD) activity were measured in hDPCs while inducible nitric oxide synthase (iNOS) and melatonin protein expression were measured in human dental pulp as well, by RT-PCR, by ELISA, and spectrophotometrically. Bioinformatic analyses were performed by using the ShinyGO (v.0.75) application. Type 2 diabetic patients showed a higher incidence of postoperative sensitivity and lower melatonin and higher iNOS content in dental pulp tissue compared with non-diabetic patients. Melatonin, when co-added in hDPC culture, reverses HEMA and CQ cytotoxic effects via anti-apoptotic and anti-inflammatory/antioxidant iNOS-related effects. Enrichment analyses showed that genes/proteins, altered by HEMA and CQ and normalized by melatonin, are the most prominently overrepresented in type 2 diabetes mellitus pathways and that they share subcellular localization in different oligomeric protein complexes consisting of anti- and pro-apoptotic regulators. This is the first evidence of the ability of melatonin to counteract iNOS-mediated inflammatory and stress effects in HEMA- and CQ-treated hDPCs, which could be of significance for the modulation of presently observed immediate postoperative sensitivity after composite restoration in type 2 diabetic patients.
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معلومات مُعتمدة: 451-03-9/2021-14/200129 Ministry of Education, Science and Technological Development of the Republic of Serbia
فهرسة مساهمة: Keywords: HEMA; apoptosis; camphorquinone; composite resin; dental pulp; melatonin; postoperative sensitivity; type 2 diabetes
المشرفين على المادة: JL5DK93RCL (Melatonin)
0 (Methacrylates)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RAL3591W33 (camphorquinone)
0 (Antioxidants)
0 (Proto-Oncogene Proteins c-bcl-2)
تواريخ الأحداث: Date Created: 20230211 Date Completed: 20230214 Latest Revision: 20230214
رمز التحديث: 20230214
مُعرف محوري في PubMed: PMC9916734
DOI: 10.3390/ijms24032562
PMID: 36768884
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms24032562