Application of Novel Breast Biospecimen Cell-Type Adjustment Identifies Shared DNA Methylation Alterations in Breast Tissue and Milk with Breast Cancer-Risk Factors.

التفاصيل البيبلوغرافية
العنوان:	Application of Novel Breast Biospecimen Cell-Type Adjustment Identifies Shared DNA Methylation Alterations in Breast Tissue and Milk with Breast Cancer-Risk Factors.
المؤلفون:	Muse ME; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire., Carroll CD; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire., Salas LA; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire., Karagas MR; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire., Christensen BC; Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire.; Department of Molecular and Systems Biology, Dartmouth Geisel School of Medicine, Hanover, New Hampshire.; Department of Community and Family Medicine, Dartmouth Geisel School of Medicine, Hanover, New Hampshire.
المصدر:	Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology [Cancer Epidemiol Biomarkers Prev] 2023 Apr 03; Vol. 32 (4), pp. 550-560.
نوع المنشور:	Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural
اللغة:	English
بيانات الدورية:	Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 9200608 Publication Model: Print Cited Medium: Internet ISSN: 1538-7755 (Electronic) Linking ISSN: 10559965 NLM ISO Abbreviation: Cancer Epidemiol Biomarkers Prev Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Philadelphia, PA : American Association for Cancer Research, c1991-
مواضيع طبية MeSH:	DNA Methylation* , Breast Neoplasms*/genetics, Female ; Humans ; Animals ; Milk ; Epigenesis, Genetic ; CpG Islands/genetics ; Risk Factors ; Gene Expression Regulation, Neoplastic
مستخلص:	Background: DNA methylation patterning is cell-type-specific and altered DNA methylation is well established to occur early in breast carcinogenesis, affecting non-cancerous, histopathologically normal breast tissue. Previous work assessing risk factor-associated alterations to DNA methylation in breast tissue has been limited, with even less published research in breast milk, a noninvasively obtained biospecimen containing sloughed mammary epithelial cells that may identify early alterations indicative of cancer risk. Methods: Here, we present a novel library for the estimation of the cellular composition of breast tissue and milk and subsequent assessment of cell-type-independent alterations to DNA methylation associated with established breast cancer-risk factors in solid breast tissue (n = 95) and breast milk (n = 48) samples using genome-scale DNA methylation measures from the Illumina HumanMethylation450 array. Results: We identified 772 hypermethylated CpGs (P < 0.01) associated with age consistent between breast tissue and breast milk samples. Age-associated hypermethylated CpG loci were significantly enriched for CpG island shore regions known to be important for regulating gene expression. Among the overlapping hypermethylated loci mapping to genes, a differentially methylated region was identified in the promoter region of SFRP2, a gene observed to undergo promoter hypermethylation in breast cancer. Conclusions: Our findings suggest the potential to identify epigenetic biomarkers of breast cancer risk in noninvasively obtained, tissue-specific breast milk specimens. Impact: This work demonstrates the potential of using breast milk as a noninvasive biomarker of breast cancer risk, improving our ability to detect early-stage disease and lowering the overall disease burden. (©2023 The Authors; Published by the American Association for Cancer Research.)
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معلومات مُعتمدة:	UH3 OD023275 United States OD NIH HHS; P30 CA023108 United States CA NCI NIH HHS; R01 CA216265 United States CA NCI NIH HHS; T32 CA134286 United States CA NCI NIH HHS; P01 ES022832 United States ES NIEHS NIH HHS; R01 CA253976 United States CA NCI NIH HHS; P20 GM104416 United States GM NIGMS NIH HHS
تواريخ الأحداث:	Date Created: 20230213 Date Completed: 20230404 Latest Revision: 20230513
رمز التحديث:	20240829
مُعرف محوري في PubMed:	PMC10068446
DOI:	10.1158/1055-9965.EPI-22-0405
PMID:	36780234
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1538-7755
DOI:	10.1158/1055-9965.EPI-22-0405