دورية أكاديمية
Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 ( OmpP2 ) of Glaesserella ( Haemophilus ) parasuis Isolates in Central State of Peninsular Malaysia.
| العنوان: | Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 ( OmpP2 ) of Glaesserella ( Haemophilus ) parasuis Isolates in Central State of Peninsular Malaysia. |
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| المؤلفون: | Lee CY; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia., Ong HX; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia., Tan CY; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia., Low SE; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia., Phang LY; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia., Lai J; Department of Veterinary Medicine, College of Veterinary Medicine, National Chiayi University, Chiayi City 60004, Taiwan., Ooi PT; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia., Fong MWC; Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia. |
| المصدر: | Pathogens (Basel, Switzerland) [Pathogens] 2023 Feb 12; Vol. 12 (2). Date of Electronic Publication: 2023 Feb 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Print ISSN: 2076-0817 (Print) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, 2012- |
| مستخلص: | Glaesserella (Haemophilus) parasuis , the etiological agent of Glässer's disease, is an economically significant pathogen commonly associated with serofibrinous polyserositis, arthritis, fibrinous bronchopneumonia and/or meningitis. This study is the first attempt to molecularly characterize and provide a detailed overview of the genetic variants of G. parasuis present in Malaysia, in reference to its serotype, virulence-associated trimeric autotransporters ( vtaA ) gene and outer membrane protein P2 ( OmpP2 ) gene. The G. parasuis isolates ( n = 11) from clinically sick field samples collected from two major pig producing states (Selangor and Perak) were selected for analysis. Upon multiplex PCR, the majority of the isolates (eight out of 11) were identified to be serotype 5 or 12, and interestingly, serotypes 3, 8 and 15 were also detected, which had never been reported in Malaysia prior to this. Generally, virulent vtaA was detected for all isolates, except for one, which displayed a nonvirulent vtaA . A phylogenetic analysis of the OmpP2 gene revealed that the majority of Malaysian isolates were clustered into genotype 1, which could be further divided into Ia and Ib, while only one isolate was clustered into genotype 2. |
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| فهرسة مساهمة: | Keywords: Glaesserella (Haemophilus) parasuis; Malaysia; outer membrane protein (OmpP2); phylogenetic tree |
| تواريخ الأحداث: | Date Created: 20230225 Latest Revision: 20230228 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9966854 |
| DOI: | 10.3390/pathogens12020308 |
| PMID: | 36839580 |
| قاعدة البيانات: | MEDLINE |
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