دورية أكاديمية
A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia.
| العنوان: | A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia. |
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| المؤلفون: | Ostuni A; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100 Potenza, Italy. Electronic address: angela.ostuni@unibas.it., Iovane V; Dipartimento di Agraria - Università degli Studi di Napoli Federico II -Via Università, 100 - 80055 Portici, NA, Italy., Monné M; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100 Potenza, Italy., Crudele MA; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100 Potenza, Italy., Scicluna MT; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova, 1411, 00178 Roma, Italy., Nardini R; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova, 1411, 00178 Roma, Italy., Raimondi P; OneHEco APS, 84047 Capaccio Paestum, SA, Italy., Frontoso R; OneHEco APS, 84047 Capaccio Paestum, SA, Italy; Istituto Zooprofilattico Sperimentale del Mezzogiorno Via Salute, 2 - 80055 Portici, Napoli, Italy., Boni R; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100 Potenza, Italy., Bavoso A; Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100 Potenza, Italy. |
| المصدر: | Journal of virological methods [J Virol Methods] 2023 May; Vol. 315, pp. 114704. Date of Electronic Publication: 2023 Feb 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Equine Infectious Anemia*/diagnosis , Infectious Anemia Virus, Equine*/genetics, Horses ; Animals ; Capsid Proteins ; Serologic Tests/veterinary ; Peptides |
| مستخلص: | Lentiviruses, including equine infectious anemia virus (EIAV), are considered viral quasispecies because of their intrinsic genetic, structural and phenotypic variability. Immunoenzymatic tests (ELISA) for EIAV reported in the literature were obtained mainly by using the capsid protein p26, which is derived almost exclusively from a single strain (Wyoming), and do not reflect the great potential epitopic variability of the EIAV quasispecies. In this investigation, the GenBank database was exploited in a systematic approach to design a set of representative protein antigens useful for EIAV serodiagnosis. The main bioinformatic tools used were clustering, molecular modelling, epitope predictions and aggregative/ solubility predictions. This approach led to the design of two antigenic proteins, i.e. a full sequence p26 capsid protein and a doublestrain polypeptide derived from the gp45 transmembrane protein fused to Maltose Binding Protein (MBP) that were expressed by recombinant DNA technology starting from synthetic genes, and analyzed by circular dichroism (CD) spectroscopy. Both proteins were used in an indirect ELISA test that can address some of the high variability of EIAV. The novel addition of the gp45 double-strain antigen contributed to enhance the diagnostic sensitivity and could be also useful for immunoblotting application. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: EIAV, Antigen design; ELISA test; Modelling and structural analysis; Protein clustering; Synthetic gene |
| المشرفين على المادة: | 0 (Capsid Proteins) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20230226 Date Completed: 20230328 Latest Revision: 20230403 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.jviromet.2023.114704 |
| PMID: | 36842487 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0984 |
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| DOI: | 10.1016/j.jviromet.2023.114704 |