دورية أكاديمية
The co-inhibitory receptor TIGIT regulates NK cell function and is upregulated in human intrahepatic CD56 bright NK cells.
| العنوان: | The co-inhibitory receptor TIGIT regulates NK cell function and is upregulated in human intrahepatic CD56 bright NK cells. |
|---|---|
| المؤلفون: | Ziegler AE; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany.; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Fittje P; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Müller LM; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Ahrenstorf AE; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Hagemann K; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Hagen SH; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Hess LU; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Niehrs A; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Poch T; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Ravichandran G; Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Löbl SM; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Padoan B; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Brias S; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany.; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Hennesen J; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Richard M; University of Bordeaux, Institut National de la Santé et de la Recherche Médicale, Bordeaux Population Health Research Center, UMR1219 and Inria, Team Statistics in systems biology and translationnal medicine (SISTM), Bordeaux, France., Richert L; University of Bordeaux, Institut National de la Santé et de la Recherche Médicale, Bordeaux Population Health Research Center, UMR1219 and Inria, Team Statistics in systems biology and translationnal medicine (SISTM), Bordeaux, France., Peine S; Institute for Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Oldhafer KJ; Department of General and Abdominal Surgery, Asklepios Hospital Barmbek, Semmelweis University of Medicine, Hamburg, Germany., Fischer L; Department of Visceral Transplant Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Schramm C; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.; Martin Zeitz Centre for Rare Diseases, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Martrus G; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Bunders MJ; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany.; III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Altfeld M; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany., Lunemann S; Research Department Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany. |
| المصدر: | Frontiers in immunology [Front Immunol] 2023 Feb 09; Vol. 14, pp. 1117320. Date of Electronic Publication: 2023 Feb 09 (Print Publication: 2023). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Killer Cells, Natural*/metabolism , Liver*/metabolism, Humans ; CD56 Antigen/metabolism ; Receptors, Immunologic/genetics ; Receptors, Immunologic/metabolism ; Flow Cytometry |
| مستخلص: | The crosstalk between NK cells and their surrounding environment is enabled through activating and inhibitory receptors, which tightly control NK cell activity. The co-inhibitory receptor TIGIT decreases NK cell cytotoxicity and is involved in NK cell exhaustion, but has also been associated with liver regeneration, highlighting that the contribution of human intrahepatic CD56 bright NK cells in regulating tissue homeostasis remains incompletely understood. A targeted single-cell mRNA analysis revealed distinct transcriptional differences between matched human peripheral blood and intrahepatic CD56 bright NK cells. Multiparameter flow cytometry identified a cluster of intrahepatic NK cells with overlapping high expression of CD56, CD69, CXCR6, TIGIT and CD96. Intrahepatic CD56 bright NK cells also expressed significantly higher protein surface levels of TIGIT, and significantly lower levels of DNAM-1 compared to matched peripheral blood CD56 bright NK cells. TIGIT + CD56 bright NK cells showed diminished degranulation and TNF-α production following stimulation. Co-incubation of peripheral blood CD56 bright NK cells with human hepatoma cells or primary human hepatocyte organoids resulted in migration of NK cells into hepatocyte organoids and upregulation of TIGIT and downregulation of DNAM-1 expression, in line with the phenotype of intrahepatic CD56 bright NK cells. Intrahepatic CD56 bright NK cells represent a transcriptionally, phenotypically, and functionally distinct population of NK cells that expresses higher levels of TIGIT and lower levels of DNAM-1 than matched peripheral blood CD56 bright NK cells. Increased expression of inhibitory receptors by NK cells within the liver environment can contribute to tissue homeostasis and reduction of liver inflammation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Ziegler, Fittje, Müller, Ahrenstorf, Hagemann, Hagen, Hess, Niehrs, Poch, Ravichandran, Löbl, Padoan, Brias, Hennesen, Richard, Richert, Peine, Oldhafer, Fischer, Schramm, Martrus, Bunders, Altfeld and Lunemann.) |
| References: | Semin Immunol. 2017 Jun;31:3-10. (PMID: 28802693) Eur J Immunol. 2019 May;49(5):758-769. (PMID: 30785638) Nat Immunol. 2013 Oct;14(10):996-1006. (PMID: 24048121) Immunity. 2014 Feb 20;40(2):225-34. (PMID: 24440149) Immunity. 2016 May 17;44(5):989-1004. (PMID: 27192565) Nat Commun. 2018 Oct 22;9(1):4383. (PMID: 30348985) Immunity. 2018 Jun 19;48(6):1172-1182.e6. (PMID: 29858012) J Clin Invest. 2013 Apr;123(4):1444-56. (PMID: 23524967) J Immunol. 2016 Dec 1;197(11):4283-4291. (PMID: 27798170) Front Oncol. 2020 Aug 07;10:1595. (PMID: 32903786) J Exp Med. 2003 Aug 18;198(4):557-67. (PMID: 12913096) Semin Immunopathol. 2021 Aug;43(4):519-533. (PMID: 34230995) Front Oncol. 2019 Feb 11;9:51. (PMID: 30805309) J Immunol. 2004 Apr 1;172(7):3994-8. (PMID: 15034010) Cell. 2015 Jan 15;160(1-2):299-312. (PMID: 25533785) Clin Cancer Res. 2020 Oct 15;26(20):5520-5533. (PMID: 32591463) PLoS Pathog. 2022 Jun 24;18(6):e1010572. (PMID: 35749424) Nat Commun. 2017 Nov 13;8(1):1443. (PMID: 29129929) Nat Biotechnol. 2013 Jun;31(6):545-52. (PMID: 23685480) Cancer Res. 2008 Oct 15;68(20):8437-45. (PMID: 18922917) Eur J Immunol. 2016 Sep;46(9):2111-20. (PMID: 27485474) J Biol Chem. 2011 Jul 8;286(27):23823-30. (PMID: 21572041) PLoS One. 2017 Aug 9;12(8):e0182532. (PMID: 28792982) Immunology. 2010 Dec;131(4):451-8. (PMID: 21039469) J Immunol. 2015 Aug 1;195(3):965-72. (PMID: 26091716) Cell Rep. 2020 Dec 8;33(10):108485. (PMID: 33296655) PLoS One. 2018 Jul 20;13(7):e0201170. (PMID: 30028872) J Exp Med. 2008 Dec 22;205(13):2959-64. (PMID: 19029379) Liver Transpl. 2010 Jul;16(7):895-908. (PMID: 20583081) Sci Rep. 2016 May 23;6:26157. (PMID: 27210614) J Exp Med. 2019 Jun 3;216(6):1311-1327. (PMID: 31040185) Science. 2007 Apr 13;316(5822):295-8. (PMID: 17363629) Front Immunol. 2018 Oct 11;9:2324. (PMID: 30364222) J Immunother Cancer. 2020 Jun;8(1):. (PMID: 32503945) Nat Immunol. 2010 Dec;11(12):1127-35. (PMID: 20972432) J Innate Immun. 2011;3(3):216-26. (PMID: 21454962) Hepatology. 2008 Feb;47(2):729-36. (PMID: 18167066) Hepatology. 2014 Oct;60(4):1389-98. (PMID: 24912841) Sci Rep. 2017 Jul 27;7(1):6676. (PMID: 28751776) Nat Immunol. 2009 Jan;10(1):48-57. (PMID: 19011627) Sci Rep. 2021 Jan 15;11(1):1551. (PMID: 33452360) BMC Cancer. 2004 Oct 07;4:73. (PMID: 15471548) Nature. 2019 Nov;575(7783):512-518. (PMID: 31597160) Hepatol Commun. 2020 Jan 06;4(3):409-424. (PMID: 32140657) Hepatology. 2019 Jul;70(1):168-183. (PMID: 30411378) J Immunol. 2004 Dec 1;173(11):6547-63. (PMID: 15557145) Front Immunol. 2018 Aug 22;9:1829. (PMID: 30186282) Immunology. 2011 May;133(1):62-73. (PMID: 21320123) Front Immunol. 2019 Apr 30;10:946. (PMID: 31114585) Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17858-63. (PMID: 19815499) Eur J Immunol. 2009 Mar;39(3):695-703. (PMID: 19197944) Nat Commun. 2019 Aug 26;10(1):3841. (PMID: 31451696) J Hepatol. 1998 Jan;28(1):84-90. (PMID: 9537869) Nat Immunol. 2004 Dec;5(12):1260-5. (PMID: 15531883) Front Immunol. 2017 Mar 20;8:301. (PMID: 28373874) J Immunol. 2015 Mar 15;194(6):2467-71. (PMID: 25672754) Front Immunol. 2019 May 07;10:909. (PMID: 31134055) Nat Immunol. 2018 Jul;19(7):723-732. (PMID: 29915296) Mol Cell Proteomics. 2019 Nov;18(11):2310-2323. (PMID: 31308249) STAR Protoc. 2021 Jul 20;2(3):100641. (PMID: 34355200) J Immunol Res. 2015;2015:839684. (PMID: 26543875) Front Immunol. 2019 Jul 03;10:1502. (PMID: 31333651) Nat Rev Immunol. 2016 Apr 28;16(5):310-20. (PMID: 27121652) Cells. 2020 Mar 21;9(3):. (PMID: 32245149) Immunology. 2009 Apr;126(4):458-65. (PMID: 19278419) Annu Rev Immunol. 2013;31:163-94. (PMID: 23298212) Front Immunol. 2021 Mar 22;12:649311. (PMID: 33828559) Nature. 2019 Aug;572(7768):199-204. (PMID: 31292543) Nat Med. 2006 Sep;12(9):1065-74. (PMID: 16892062) J Exp Med. 2008 Dec 22;205(13):2965-73. (PMID: 19029380) Nat Immunol. 2008 May;9(5):503-10. (PMID: 18425107) PLoS Pathog. 2019 Jun 20;15(6):e1007797. (PMID: 31220194) Nat Immunol. 2014 May;15(5):431-8. (PMID: 24658051) Methods. 2013 Jan;59(1):71-9. (PMID: 23079396) Front Immunol. 2019 Jun 07;10:1247. (PMID: 31231382) Blood. 1998 Dec 15;92(12):4602-11. (PMID: 9845526) J Immunol. 2009 Oct 15;183(8):4921-30. (PMID: 19801517) J Autoimmun. 2016 Jan;66:40-50. (PMID: 26330348) Immunology. 2011 Jul;133(3):350-9. (PMID: 21506999) |
| فهرسة مساهمة: | Keywords: DNAM-1; PVR/CD155; TIGIT; immune tolerance; intrahepatic NK cells; liver organoids; single-cell mRNA analysis; tissue homeostasis |
| المشرفين على المادة: | 0 (CD56 Antigen) 0 (Receptors, Immunologic) 0 (TIGIT protein, human) |
| تواريخ الأحداث: | Date Created: 20230227 Date Completed: 20230228 Latest Revision: 20240314 |
| رمز التحديث: | 20240315 |
| مُعرف محوري في PubMed: | PMC9948018 |
| DOI: | 10.3389/fimmu.2023.1117320 |
| PMID: | 36845105 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
|---|---|
| DOI: | 10.3389/fimmu.2023.1117320 |