دورية أكاديمية
أعرض في EDS

Umbilical cord blood-derived exosomes from healthy term pregnancies protect against hyperoxia-induced lung injury in mice.

التفاصيل البيبلوغرافية
العنوان: Umbilical cord blood-derived exosomes from healthy term pregnancies protect against hyperoxia-induced lung injury in mice.
المؤلفون: Zhong XQ; Department of Neonatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.; Key Laboratory for Major Obstetric Disease of Guangdong Province, Guangzhou, China., Wang D; Key Laboratory for Major Obstetric Disease of Guangdong Province, Guangzhou, China.; Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Chen S; Center for Translational Medicine, Institute of Precision Medicine, Department of Medical Oncology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China., Zheng J; Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin, USA., Hao TF; Department of Biochemistry and Molecular Biology, Sun Yat-Sen University Zhongshan School of Medicine, Guangzhou, China., Li XH; Department of Maternal and Child Health, School of Public Health, Sun Yat-sen University, Guangzhou, China., Luo LH; Department of Neonatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Gu J; Department of Neonatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Lian CY; Department of Neonatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Li XS; Key Laboratory of Cardiovascular Diseases, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China., Chen DJ; Key Laboratory for Major Obstetric Disease of Guangdong Province, Guangzhou, China.; Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
المصدر: Clinical and translational science [Clin Transl Sci] 2023 Jun; Vol. 16 (6), pp. 966-977. Date of Electronic Publication: 2023 Mar 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: WileyBlackwell Pub Country of Publication: United States NLM ID: 101474067 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1752-8062 (Electronic) Linking ISSN: 17528054 NLM ISO Abbreviation: Clin Transl Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Malden, MA : WileyBlackwell Pub., 2008-
مواضيع طبية MeSH: Lung Injury*/etiology , Lung Injury*/prevention & control , Hyperoxia*/complications , Exosomes*/pathology , Bronchopulmonary Dysplasia*/etiology , Bronchopulmonary Dysplasia*/prevention & control , MicroRNAs*/genetics, Infant, Newborn ; Pregnancy ; Female ; Animals ; Mice ; Humans ; Animals, Newborn ; Endothelial Cells/pathology ; Fetal Blood ; Lung/pathology
مستخلص: Bronchopulmonary dysplasia (BPD) is a chronic, devastating disease primarily occurring in premature infants. To date, intervention strategies to prevent or treat BPD are limited. We aimed to determine the effects of umbilical cord blood-derived exosomes (UCB-EXOs) from healthy term pregnancies on hyperoxia-induced lung injury and to identify potential targets for BPD intervention. A mouse model of hyperoxia-induced lung injury was created by exposing neonatal mice to hyperoxia after birth until the 14th day post birth. Age-matched neonatal mice were exposed to normoxia as the control. Hyperoxia-induced lung injury mice were intraperitoneally injected with UCB-EXO or vehicle daily for 3 days, starting on day 4 post birth. Human umbilical vein endothelial cells (HUVECs) were insulted with hyperoxia to establish an in vitro model of BPD to investigate angiogenesis dysfunction. Our results showed that UCB-EXO alleviated lung injuries in hyperoxia-insulted mice by reducing histopathological grade and collagen contents in the lung tissues. UCB-EXO also promoted vascular growth and increased miR-185-5p levels in the lungs of hyperoxia-insulted mice. Additionally, we found that UCB-EXO elevated miR-185-5p levels in HUVECs. MiR-185-5p overexpression inhibited cell apoptosis, whereas promoted cell migration in HUVECs exposed to hyperoxia. The luciferase reporter assay results revealed that miR-185-5p directly targeted cyclin-dependent kinase 6 (CDK6), which was downregulated in the lungs of hyperoxia-insulted mice. Together, these data suggest that UCB-EXO from healthy term pregnancies protect against hyperoxia-induced lung injuries via promoting neonatal pulmonary angiogenesis partially by elevating miR-185-5p.
(© 2023 The Authors. Clinical and Translational Science published by Wiley Periodicals LLC on behalf of American Society for Clinical Pharmacology and Therapeutics.)
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المشرفين على المادة: 0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20230304 Date Completed: 20230615 Latest Revision: 20230624
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10264931
DOI: 10.1111/cts.13502
PMID: 36869608
قاعدة البيانات: MEDLINE
الوصف
تدمد:1752-8062
DOI:10.1111/cts.13502