دورية أكاديمية
Local calcium signal transmission in mycelial network exhibits decentralized stress responses.
العنوان: | Local calcium signal transmission in mycelial network exhibits decentralized stress responses. |
---|---|
المؤلفون: | Itani A; Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan., Masuo S; Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan., Yamamoto R; Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan., Serizawa T; Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan., Fukasawa Y; Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko, Osaki, Miyagi, 989-6711, Japan., Takaya N; Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan., Toyota M; Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama, 338-8570, Japan.; Suntory Rising Stars Encouragement Program in Life Sciences (SunRiSE), Kyoto, Japan.; Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison, WI 53706, USA., Betsuyaku S; Department of Plant Life Science, Faculty of Agriculture, Ryukoku University, 1-5 Yokotani, Seta Oe-cho, Otsu, Shiga, 520-2194Japan., Takeshita N; Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan. |
المصدر: | PNAS nexus [PNAS Nexus] 2023 Mar 07; Vol. 2 (3), pp. pgad012. Date of Electronic Publication: 2023 Mar 07 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press on behalf of the National Academy of Sciences Country of Publication: England NLM ID: 9918367777906676 Publication Model: eCollection Cited Medium: Internet ISSN: 2752-6542 (Electronic) Linking ISSN: 27526542 NLM ISO Abbreviation: PNAS Nexus Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Oxford] : Oxford University Press on behalf of the National Academy of Sciences, [2022]- |
مستخلص: | Many fungi live as mycelia, which are networks of hyphae. Mycelial networks are suited for the widespread distribution of nutrients and water. The logistical capabilities are critical for the extension of fungal survival areas, nutrient cycling in ecosystems, mycorrhizal symbioses, and virulence. In addition, signal transduction in mycelial networks is predicted to be vital for mycelial function and robustness. A lot of cell biological studies have elucidated protein and membrane trafficking and signal transduction in fungal hyphae; however, there are no reports visualizing signal transduction in mycelia. This paper, by using the fluorescent Ca 2+ biosensor, visualized for the first time how calcium signaling is conducted inside the mycelial network in response to localized stimuli in the model fungus Aspergillus nidulans . The wavy propagation of the calcium signal inside the mycelium or the signal blinking in the hyphae varies depending on the type of stress and proximity to the stress. The signals, however, only extended around 1,500 μm, suggesting that the mycelium has a localized response. The mycelium showed growth delay only in the stressed areas. Local stress caused arrest and resumption of mycelial growth through reorganization of the actin cytoskeleton and membrane trafficking. To elucidate the downstream of calcium signaling, calmodulin, and calmodulin-dependent protein kinases, the principal intracellular Ca 2+ receptors were immunoprecipitated and their downstream targets were identified by mass spectrometry analyses. Our data provide evidence that the mycelial network, which lacks a brain or nervous system, exhibits decentralized response through locally activated calcium signaling in response to local stress. (© The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences.) |
References: | AoB Plants. 2015 May 15;7:. (PMID: 25979966) Mol Microbiol. 1999 Dec;34(5):1049-57. (PMID: 10594829) Fungal Genet Biol. 2018 Jan;110:10-14. (PMID: 29229585) Sci Adv. 2018 Jan 24;4(1):e1701798. (PMID: 29387789) Fungal Genet Biol. 2009 Mar;46 Suppl 1:S82-S92. (PMID: 19610202) Nat Methods. 2009 Jan;6(1):79-81. (PMID: 19079252) Microbiol Mol Biol Rev. 2015 Jun;79(2):243-62. (PMID: 25971588) Science. 2018 Sep 14;361(6407):1112-1115. (PMID: 30213912) ISME J. 2020 Feb;14(2):380-388. (PMID: 31628441) Nature. 2002 Nov 14;420(6912):238-45. (PMID: 12432409) Fungal Genet Biol. 2021 Jun;151:103470. (PMID: 32979514) Annu Rev Physiol. 1994;56:169-91. (PMID: 8010739) Mol Biol Cell. 1996 Oct;7(10):1511-9. (PMID: 8898358) Fungal Genet Biol. 2021 Apr;149:103540. (PMID: 33607281) R Soc Open Sci. 2022 Apr 6;9(4):211926. (PMID: 35425630) J Cell Sci. 1994 Jun;107 ( Pt 6):1519-28. (PMID: 7962194) Chem Rev. 2001 Aug;101(8):2341-52. (PMID: 11749376) J Biol Chem. 2000 Dec 8;275(49):38230-8. (PMID: 10988293) New Phytol. 2009;182(2):314-330. (PMID: 19236579) PLoS One. 2012;7(2):e31218. (PMID: 22359575) Nat Microbiol. 2022 May;7(5):607-619. (PMID: 35508719) Mol Microbiol. 2010 Dec;78(5):1058-76. (PMID: 21091496) Eukaryot Cell. 2009 Jul;8(7):945-56. (PMID: 19411617) Biochim Biophys Acta Mol Cell Res. 2021 Apr;1868(4):118948. (PMID: 33421535) Fungal Genet Biol. 2010 Mar;47(3):268-78. (PMID: 20034586) FEMS Microbiol Lett. 2002 Apr 23;210(1):61-6. (PMID: 12023078) J Cell Sci. 1997 Aug;110 ( Pt 15):1805-12. (PMID: 9264467) Microbiol Mol Biol Rev. 2018 Apr 11;82(2):. (PMID: 29643171) Nat Rev Mol Cell Biol. 2000 Oct;1(1):11-21. (PMID: 11413485) Curr Opin Neurobiol. 2005 Jun;15(3):266-74. (PMID: 15919191) Cell Motil Cytoskeleton. 2000 Feb;45(2):163-72. (PMID: 10658211) Eukaryot Cell. 2003 Dec;2(6):1169-77. (PMID: 14665452) J Vis Exp. 2021 Jun 4;(172):. (PMID: 34152317) Dev Growth Differ. 2009 Dec;51(9):769-75. (PMID: 19843153) Mycopathologia. 2014 Dec;178(5-6):325-30. (PMID: 24952717) Proc Natl Acad Sci U S A. 2017 May 30;114(22):5701-5706. (PMID: 28507141) Genes Dev. 1999 Apr 1;13(7):798-803. (PMID: 10197980) Microbiol Spectr. 2017 May;5(3):. (PMID: 28524023) Nat Commun. 2017 Feb 08;8:14444. (PMID: 28176784) Virulence. 2017 Feb 17;8(2):186-197. (PMID: 27325145) J Cell Sci. 2010 Jul 1;123(Pt 13):2199-206. (PMID: 20530569) Traffic. 2017 Nov;18(11):711-719. (PMID: 28846184) Mol Plant Pathol. 2012 May;13(4):414-30. (PMID: 22471698) ISME J. 2021 Feb;15(2):435-449. (PMID: 32989245) Biochim Biophys Acta. 2014 Mar;1840(3):964-9. (PMID: 24269537) Science. 1995 Apr 14;268(5208):239-47. (PMID: 7716515) Mycologia. 2002 Mar-Apr;94(2):260-6. (PMID: 21156495) Mol Biol Cell. 2009 Jan;20(2):673-84. (PMID: 19037104) Curr Opin Plant Biol. 2015 Aug;26:100-5. (PMID: 26190588) J Cell Biol. 1993 May;121(3):621-30. (PMID: 8486741) PLoS One. 2013;8(3):e58008. (PMID: 23505451) |
فهرسة مساهمة: | Keywords: calcium signal; calmodulin; calmodulin-dependent kinases; fungi; mycelia |
تواريخ الأحداث: | Date Created: 20230310 Latest Revision: 20230311 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9991499 |
DOI: | 10.1093/pnasnexus/pgad012 |
PMID: | 36896124 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!