دورية أكاديمية
Detection of Toxoplasma gondii DNA in milk of dairy cows from southern Brazil.
العنوان: | Detection of Toxoplasma gondii DNA in milk of dairy cows from southern Brazil. |
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المؤلفون: | da Fonseca FM; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil. Electronic address: flaviamoreiravet@gmail.com., Sato AP; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil., Becker APBB; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil., da Paz Augusto Pinto GO; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil., de Souza GS; Autonomous Veterinarian, Rua Curupis, 1522, Curitiba, Paraná IN 80330-040, Brazil., Perotta JH; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil., de Barros Filho IR; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil., Rodriguez MC; Marcos Enrietti Diagnostic Center, Parana State Agency for Agricultural Defense, R. Jaime Balão, 575, 80040-340 Curitiba, Paraná, Brazil., Locatelli-Dittrich R; Department of Veterinary Medicine, Federal University of Paraná, Rua dos Funcionários 1540, CEP 80035-050 Curitiba, Paraná, Brazil. Electronic address: roslocdi@ufpr.br. |
المصدر: | Parasitology international [Parasitol Int] 2023 Aug; Vol. 95, pp. 102750. Date of Electronic Publication: 2023 Mar 14. |
نوع المنشور: | Journal Article; Randomized Controlled Trial, Veterinary |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9708549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0329 (Electronic) Linking ISSN: 13835769 NLM ISO Abbreviation: Parasitol Int Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c1997- |
مواضيع طبية MeSH: | Toxoplasma*/genetics , Toxoplasmosis, Animal*/parasitology, Animals ; Cattle ; Female ; Antibodies, Protozoan ; Brazil/epidemiology ; DNA, Protozoan/analysis ; Immunoglobulin G ; Milk/parasitology ; Seroepidemiologic Studies |
مستخلص: | Consumption of unpasteurized cow's milk may be a transmission route for some pathogenic microorganisms, but there is little information about the risk of Toxoplasma gondii infection. Blood and milk samples were collected in a paired and random fashion from 106 dairy cows and bulk-tank milk samples were also collected from each of the six farms, in southern Brazil. Serum anti-T.gondii antibodies (IgG) were detected by an indirect fluorescent antibody test (IFAT) with a cutoff point of 1:64. Nested PCR targeting the ITS1 was performed on milk samples to detect the Sarcocystidae family, confirmed to be T.gondii by Sanger sequencing. The occurrence of anti-T.gondii antibodies in the herds was 14.1%, (15/106) with seropositive cows in all herds. Antibody titers in positive samples ranged from 64 to 128. T.gondii DNA was detected in 2.8% (03/106) of the milk samples. The ITS1 sequences generated in this study were ON809793 - ON809794 and the sequencing revealed 98-100% identity with T. gondii DNA sequences deposited in GenBank. All cows PCR positive for T.gondii in milk were negative for IgG antibodies in serum, suggesting that naturally infected cows may shed T. gondii in milk in the acute phase of infection. The results of this study demonstrate that T. gondii DNA may be detected in raw cow's milk, so the potential risks of lactogenic infection should be considered. The presence of T. gondii DNA in milk does not confirm that the protozoa are viable and infective, and further investigations into the role of cow's milk in the epidemiology of toxoplasmosis are needed. Competing Interests: Declaration of Competing Interest None. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
فهرسة مساهمة: | Keywords: Dairy cattle; IFAT; Nested PCR; Raw milk; Toxoplasmosis |
المشرفين على المادة: | 0 (Antibodies, Protozoan) 0 (DNA, Protozoan) 0 (Immunoglobulin G) |
تواريخ الأحداث: | Date Created: 20230316 Date Completed: 20230531 Latest Revision: 20230531 |
رمز التحديث: | 20231215 |
DOI: | 10.1016/j.parint.2023.102750 |
PMID: | 36925059 |
قاعدة البيانات: | MEDLINE |
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