دورية أكاديمية
Chloroacetonitrile exposure induces endoplasmic reticulum stress and affects spindle assembly in mouse oocytes.
| العنوان: | Chloroacetonitrile exposure induces endoplasmic reticulum stress and affects spindle assembly in mouse oocytes. |
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| المؤلفون: | Qiao FX; Medical College, Guangxi University, Nanning, Guangxi, 530004, China., Sun MX; Medical College, Guangxi University, Nanning, Guangxi, 530004, China., Xu ZR; Translational Medicine Research Center, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi, 530011, China., Liu YC; Medical College, Guangxi University, Nanning, Guangxi, 530004, China., Chen YZ; Medical College, Guangxi University, Nanning, Guangxi, 530004, China., Wang HL; Department of Basic Medicine, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China., Qi ZQ; Medical College, Guangxi University, Nanning, Guangxi, 530004, China., Xu CL; Reproductive Medical Center of Nanning Second People's Hospital, Nanning, Guangxi, 530031, China. Electronic address: xuchanglong2011@hotmail.com., Liu Y; Medical College, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: liuyu@gxu.edu.cn. |
| المصدر: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2023 Jun; Vol. 176, pp. 113736. Date of Electronic Publication: 2023 Mar 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8207483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6351 (Electronic) Linking ISSN: 02786915 NLM ISO Abbreviation: Food Chem Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Exeter : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, c1982- |
| مواضيع طبية MeSH: | Oocytes* , Endoplasmic Reticulum Stress*, Female ; Mice ; Animals ; Acetonitriles ; Cell Cycle |
| مستخلص: | Chloroacetonitrile (CAN) is a halogenated acetonitrile often produced while disinfecting drinking water. Previous studies have shown that maternal exposure to CAN interferes with fetal development; however, the adverse effects on maternal oocytes remain unknown. In this study, in vitro exposure of mouse oocytes to CAN reduced maturation significantly. Transcriptomics analysis showed that CAN altered the expression of multiple oocyte genes, especially those associated with the protein folding process. CAN exposure induced reactive oxygen species production, accompanied by endoplasmic reticulum (ER) stress and increased glucose regulated protein 78, C/EBP homologous protein and activating transcription factor 6 expression. Moreover, our results indicated that spindle morphology was impaired after CAN exposure. CAN disrupted polo-like kinase 1, pericentrin and p-Aurora A distribution, which may be an origin inducer that disrupts spindle assemble. Furthermore, exposure to CAN in vivo impaired follicular development. Taken together, our findings indicate that CAN exposure induces ER stress and affects spindle assembly in mouse oocytes. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chloroacetonitrile; ER stress; Oocytes; Reactive oxygen species; Spindle |
| المشرفين على المادة: | CN524K9DXD (chloroacetonitrile) 0 (Acetonitriles) |
| تواريخ الأحداث: | Date Created: 20230320 Date Completed: 20230526 Latest Revision: 20230526 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.fct.2023.113736 |
| PMID: | 36940772 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6351 |
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| DOI: | 10.1016/j.fct.2023.113736 |