التفاصيل البيبلوغرافية
| العنوان: |
Smart Lattice Light Sheet Microscopy for imaging rare and complex cellular events. |
| المؤلفون: |
Shi Y, Tabet JS, Milkie DE, Daugird TA, Yang CQ, Giovannucci A, Legant WR |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Mar 09. Date of Electronic Publication: 2023 Mar 09. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Light sheet microscopes enable rapid, high-resolution imaging of biological specimens; however, biological processes span a variety of spatiotemporal scales. Moreover, long-term phenotypes are often instigated by rare or fleeting biological events that are difficult to capture with a single imaging modality and constant imaging parameters. To overcome this limitation, we present smartLLSM, a microscope that incorporates AI-based instrument control to autonomously switch between epifluorescent inverted imaging and lattice light sheet microscopy. We apply this technology to two major scenarios. First, we demonstrate that the instrument provides population-level statistics of cell cycle states across thousands of cells on a coverslip. Second, we show that by using real-time image feedback to switch between imaging modes, the instrument autonomously captures multicolor 3D datasets or 4D time-lapse movies of dividing cells at rates that dramatically exceed human capabilities. Quantitative image analysis on high-content + high-throughput datasets reveal kinetochore and chromosome dynamics in dividing cells and determine the effects of drug perturbation on cells in specific mitotic stages. This new methodology enables efficient detection of rare events within a heterogeneous cell population and records these processes with high spatiotemporal 4D imaging over statistically significant replicates. |
| التعليقات: |
Update in: Nat Methods. 2024 Jan 2;:. (PMID: 38167656) |
| تواريخ الأحداث: |
Date Created: 20230322 Latest Revision: 20240213 |
| رمز التحديث: |
20240214 |
| مُعرف محوري في PubMed: |
PMC10028917 |
| DOI: |
10.1101/2023.03.07.531517 |
| PMID: |
36945393 |
| قاعدة البيانات: |
MEDLINE |
