دورية أكاديمية
Open pathways for cerebrospinal fluid outflow at the cribriform plate along the olfactory nerves.
| العنوان: | Open pathways for cerebrospinal fluid outflow at the cribriform plate along the olfactory nerves. |
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| المؤلفون: | Spera I; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Cousin N; Institute of Pharmaceutical Sciences, ETH Zürich, Zürich, Switzerland., Ries M; Institute of Pharmaceutical Sciences, ETH Zürich, Zürich, Switzerland., Kedracka A; Institute of Pharmaceutical Sciences, ETH Zürich, Zürich, Switzerland., Castillo A; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Aleandri S; Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Bern, Switzerland., Vladymyrov M; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Mapunda JA; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Engelhardt B; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Luciani P; Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Bern, Switzerland., Detmar M; Institute of Pharmaceutical Sciences, ETH Zürich, Zürich, Switzerland., Proulx ST; Theodor Kocher Institute, University of Bern, Bern, Switzerland. Electronic address: steven.proulx@tki.unibe.ch. |
| المصدر: | EBioMedicine [EBioMedicine] 2023 May; Vol. 91, pp. 104558. Date of Electronic Publication: 2023 Apr 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101647039 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2352-3964 (Electronic) Linking ISSN: 23523964 NLM ISO Abbreviation: EBioMedicine Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier B.V., [2014]- |
| مواضيع طبية MeSH: | Olfactory Nerve* , Lymphatic Vessels*, Animals ; Mice ; Ethmoid Bone ; Lymphatic System/metabolism ; Subarachnoid Space/metabolism |
| مستخلص: | Background: Routes along the olfactory nerves crossing the cribriform plate that extend to lymphatic vessels within the nasal cavity have been identified as a critical cerebrospinal fluid (CSF) outflow pathway. However, it is still unclear how the efflux pathways along the nerves connect to lymphatic vessels or if any functional barriers are present at this site. The aim of this study was to anatomically define the connections between the subarachnoid space and the lymphatic system at the cribriform plate in mice. Methods: PEGylated fluorescent microbeads were infused into the CSF space in Prox1-GFP reporter mice and decalcification histology was utilized to investigate the anatomical connections between the subarachnoid space and the lymphatic vessels in the nasal submucosa. A fluorescently-labelled antibody marking vascular endothelium was injected into the cisterna magna to demonstrate the functionality of the lymphatic vessels in the olfactory region. Finally, we performed immunostaining to study the distribution of the arachnoid barrier at the cribriform plate region. Findings: We identified that there are open and direct connections from the subarachnoid space to lymphatic vessels enwrapping the olfactory nerves as they cross the cribriform plate towards the nasal submucosa. Furthermore, lymphatic vessels adjacent to the olfactory bulbs form a continuous network that is functionally connected to lymphatics in the nasal submucosa. Immunostainings revealed a discontinuous distribution of the arachnoid barrier at the olfactory region of the mouse. Interpretation: Our data supports a direct bulk flow mechanism through the cribriform plate allowing CSF drainage into nasal submucosal lymphatics in mice. Funding: This study was supported by the Swiss National Science Foundation (310030_189226), Dementia Research Switzerland-Synapsis Foundation, the Heidi Seiler Stiftung and the Fondation Dr. Corinne Schuler. Competing Interests: Declaration of interests The authors declare no conflict of interests. (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Arachnoid barrier; Cerebrospinal fluid; Cribriform plate; Lymphatic vessels; Olfactory nerves |
| تواريخ الأحداث: | Date Created: 20230412 Date Completed: 20230515 Latest Revision: 20230515 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10119713 |
| DOI: | 10.1016/j.ebiom.2023.104558 |
| PMID: | 37043871 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2352-3964 |
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| DOI: | 10.1016/j.ebiom.2023.104558 |