دورية أكاديمية
Effects of Extended Cage Component Sanitation Interval on the Microenvironment, Health, and Gastrointestinal Microbiome of Rats ( Rattus norvegicus ).
| العنوان: | Effects of Extended Cage Component Sanitation Interval on the Microenvironment, Health, and Gastrointestinal Microbiome of Rats ( Rattus norvegicus ). |
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| المؤلفون: | Taylor JZ; Office of Laboratory Animal Resources, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado., Fong DL, Habenicht LM; Office of Laboratory Animal Resources, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado., Fink MK; Office of Laboratory Animal Resources, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado., Leszczynski JK; Office of Laboratory Animal Resources, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado., Frank DN; Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado., Kofonow JM; Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado., Robertson CE; Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado., Nicklawsky AG; University of Colorado Cancer Center Biostatistics Core, Aurora, Colorado., Schurr MJ; Department of Immunology and Microbiology, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado., Manuel CA; Office of Laboratory Animal Resources, University of Colorado Denver, Anschutz Medical Campus, Aurora, Colorado. |
| المصدر: | Journal of the American Association for Laboratory Animal Science : JAALAS [J Am Assoc Lab Anim Sci] 2023 May 01; Vol. 62 (3), pp. 212-221. Date of Electronic Publication: 2023 Apr 18. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Laboratory Animal Science Country of Publication: United States NLM ID: 101269489 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2769-6677 (Electronic) Linking ISSN: 15596109 NLM ISO Abbreviation: J Am Assoc Lab Anim Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Memphis, TN : American Association for Laboratory Animal Science, [2006]- |
| مواضيع طبية MeSH: | Gastrointestinal Microbiome*, Rats ; Animals ; Ammonia ; Sanitation ; Housing, Animal ; Animal Husbandry/methods |
| مستخلص: | Washing and sanitizing rodent cage components requires costly equipment, significant personnel effort, and use of natural resources. The benchmark frequency for sanitation of individually ventilated caging (IVC) has traditionally been every 2 wk. In this study, we investigated the effects of extending this interval on the cage microenvironment, basic markers of health, and the gastrointestinal microbiota of rats. We compared our institutional standard of changing the sanitation interval for rat cage lids, box feeders, and enrichment devices from every 4 wk to an interval of 12 wk. The cage bottom and bedding continued to be changed every 2 wk for both groups. We hypothesized that we would find no significant difference between our current practice of 4 wks and continuous use for 12 wk. Our data showed that intracage ammonia levels remained below 5 ppm for most cages in both groups, with the exception of cages that experienced a cage flood. We found no significant difference between groups in bacterial colony forming units (CFU) on cage components. We used 3 novel methods of assessing cleanliness of enrichment devices and found no significant effect of continuous use for 12 wk on the number of CFU. In addition, we found no significant differences between groups for animal weight, routine blood work, or fecal and cecal microbiomes. These data indicate that a sanitation interval of up to 12 wk for components of rat IVC caging has no significant effects on the microenvironment or health of rats. Using the longer interval will improve efficiency, reduce the use of natural resources, and decrease costs while maintaining high-quality animal care. |
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| معلومات مُعتمدة: | P30 CA046934 United States CA NCI NIH HHS |
| المشرفين على المادة: | 7664-41-7 (Ammonia) |
| تواريخ الأحداث: | Date Created: 20230418 Date Completed: 20230522 Latest Revision: 20231102 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10230543 |
| DOI: | 10.30802/AALAS-JAALAS-22-000113 |
| PMID: | 37072181 |
| قاعدة البيانات: | MEDLINE |
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