دورية أكاديمية
أعرض في EDS

Molecular detection and characterization of Cryptosporidium spp., Giardia duodenalis , and Enterocytozoon bieneusi infections in dromedary camels ( Camelus dromedaries ) in Egypt.

التفاصيل البيبلوغرافية
العنوان: Molecular detection and characterization of Cryptosporidium spp., Giardia duodenalis , and Enterocytozoon bieneusi infections in dromedary camels ( Camelus dromedaries ) in Egypt.
المؤلفون: Elmahallawy EK; Department of Zoonoses, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt., Köster PC; Parasitology Reference and Research Laboratory, National Centre for Microbiology, Madrid, Spain., Dashti A; Parasitology Reference and Research Laboratory, National Centre for Microbiology, Madrid, Spain., Alghamdi SQ; Department of Biology, Faculty of Science, Al-Baha University, Alaqiq, Al-Baha Province, Saudi Arabia., Saleh A; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt., Gareh A; Department of Parasitology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt., Alrashdi BM; Biology Department, College of Science, Jouf University, Sakaka, Saudi Arabia., Hernández-Castro C; Parasitology Reference and Research Laboratory, National Centre for Microbiology, Madrid, Spain.; Parasitology Group, Faculty of Medicine, Academic Corporation for the Study of Tropical Pathologies, University of Antioquia, Medellín, Colombia., Bailo B; Parasitology Reference and Research Laboratory, National Centre for Microbiology, Madrid, Spain., Lokman MS; Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt., Hassanen EAA; Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., González-Barrio D; Parasitology Reference and Research Laboratory, National Centre for Microbiology, Madrid, Spain., Carmena D; Parasitology Reference and Research Laboratory, National Centre for Microbiology, Madrid, Spain.; CIBER Infectious Diseases (CIBERINFEC), Health Institute Carlos III, Madrid, Spain.
المصدر: Frontiers in veterinary science [Front Vet Sci] 2023 Apr 20; Vol. 10, pp. 1139388. Date of Electronic Publication: 2023 Apr 20 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2014]-
مستخلص: Introduction: Few studies have investigated the occurrence of microeukaryotic gut parasites in dromedary camels in Egypt, and the majority of these investigations are based on microscopic analysis of fecal material.
Methods: Herein, we assessed the occurrence, molecular diversity, and zoonotic potential of protozoan ( Cryptosporidium spp. and Giardia duodenalis ) and microsporidian ( Enterocytozoon bieneusi ) pathogens in individual fecal samples ( n = 102) of dromedary camels with ( n = 26) and without ( n = 76) diarrhea from Aswan Governorate, Upper Egypt. Other factors possibly associated with an increased risk of infection (geographical origin, sex, age, and physical condition) were also analyzed. The SSU rRNA or ITS genes were targeted by molecular (PCR and Sanger sequencing) techniques for pathogen detection and species identification.
Results and Discussion: The most abundant species detected was G. duodenalis (3.9%, 4/102; 95% CI: 1.1-9.7), followed by Cryptosporidium spp . (2.9%, 3/102; 95% CI: 0.6-8.4). All samples tested negative for the presence of E. bieneusi . Sequence analysis data confirmed the presence of zoonotic C. parvum (66.7%, 2/3) and cattle-adapted C. bovis (33.3%, 1/3). These Cryptosporidium isolates, as well as the four Giardia -positive isolates, were unable to be amplified at adequate genotyping markers ( Cryptosporidium : gp60 ; Giardia : gdh, bg , and tpi ). Camels younger than 2 years old were significantly more likely to harbor Cryptosporidium infections. This connection was not statistically significant, although two of the three cryptosporidiosis cases were detected in camels with diarrhea. The spread of G. duodenalis infections was unaffected by any risk variables studied. This is the first report of C. parvum and C. bovis in Egyptian camels. The finding of zoonotic C. parvum has public health implications since camels may function as sources of oocyst pollution in the environment and potentially infect livestock and humans. Although preliminary, this study provides useful baseline data on the epidemiology of diarrhea-causing microeukaryotic parasites in Egypt. Further research is required to confirm and expand our findings in other animal populations and geographical regions of the country.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Elmahallawy, Köster, Dashti, Alghamdi, Saleh, Gareh, Alrashdi, Hernández-Castro, Bailo, Lokman, Hassanen, González-Barrio and Carmena.)
References: Vet Parasitol. 2000 Nov 10;93(2):103-12. (PMID: 11035228)
Parasitol Res. 2016 Aug;115(8):3197-202. (PMID: 27112756)
Parasitol Res. 2022 Jul;121(7):2087-2092. (PMID: 35579756)
Trop Parasitol. 2018 Jan-Jun;8(1):2-7. (PMID: 29930899)
Front Vet Sci. 2021 Oct 04;8:750640. (PMID: 34671663)
Clin Microbiol Rev. 2012 Jul;25(3):420-49. (PMID: 22763633)
Vet Parasitol. 2008 May 31;153(3-4):363-7. (PMID: 18343038)
Enferm Infecc Microbiol Clin (Engl Ed). 2020 Jan;38 Suppl 1:24-31. (PMID: 32111362)
PLoS Negl Trop Dis. 2021 Jan 19;15(1):e0008987. (PMID: 33465074)
Front Vet Sci. 2022 Jun 17;9:899854. (PMID: 35782567)
J Parasitol Res. 2020 Feb 18;2020:7834026. (PMID: 32148933)
Acta Trop. 2018 Aug;184:1-14. (PMID: 29111140)
Am J Vet Res. 2001 Oct;62(10):1612-5. (PMID: 11592328)
Clin Microbiol Rev. 2011 Jan;24(1):110-40. (PMID: 21233509)
Int J Parasitol. 2021 Dec;51(13-14):1099-1119. (PMID: 34715087)
Front Vet Sci. 2021 Mar 22;8:651672. (PMID: 33829053)
Acta Trop. 2018 Aug;184:15-28. (PMID: 29395034)
J Zoo Wildl Med. 2016 Sep;47(3):846-854. (PMID: 27691966)
Parasit Vectors. 2018 Apr 2;11(1):219. (PMID: 29609645)
Acta Parasitol. 2018 Jun 26;63(2):422-427. (PMID: 29654683)
Evol Appl. 2016 Jan 08;9(2):313-33. (PMID: 26834828)
J Clin Microbiol. 2006 Dec;44(12):4303-8. (PMID: 17005736)
J Egypt Soc Parasitol. 1996 Apr;26(1):143-53. (PMID: 8721235)
Parasit Vectors. 2017 Apr 20;10(1):195. (PMID: 28427454)
Parasite. 2017;24:21. (PMID: 28617666)
Vet Clin North Am Food Anim Pract. 2020 Mar;36(1):223-238. (PMID: 32029186)
J Trop Med. 2022 May 31;2022:4271063. (PMID: 35686207)
Vet World. 2020 Aug;13(8):1635-1640. (PMID: 33061238)
Parasitol Res. 2007 Oct;101(5):1443-8. (PMID: 17569991)
Clin Microbiol Rev. 2014 Apr;27(2):371-418. (PMID: 24696439)
Vet Rec. 2005 Sep 17;157(12):350-2. (PMID: 16170004)
Parasitol Res. 2018 Mar;117(3):905-910. (PMID: 29327324)
Parasitol Res. 2019 Oct;118(10):3053-3060. (PMID: 31420739)
Int J Parasitol. 2005 Feb;35(2):207-13. (PMID: 15710441)
Parasitol Int. 2022 Oct;90:102618. (PMID: 35777654)
Acta Trop. 2019 Sep;197:105060. (PMID: 31194962)
Vet Sci. 2020 Oct 27;7(4):. (PMID: 33120870)
Trop Med Int Health. 2002 Apr;7(4):357-64. (PMID: 11952952)
Appl Environ Microbiol. 2002 May;68(5):2595-9. (PMID: 11976142)
Parasitol Res. 2020 Nov;119(11):3873-3880. (PMID: 33006040)
Pathogens. 2020 Nov 13;9(11):. (PMID: 33202835)
Vet Parasitol. 2013 Mar 31;193(1-3):15-24. (PMID: 23305974)
Emerg Infect Dis. 2003 Nov;9(11):1444-52. (PMID: 14718089)
Parasitol Res. 2014 Jun;113(6):2129-36. (PMID: 24676462)
J Parasit Dis. 2015 Dec;39(4):730-5. (PMID: 26688643)
Infect Genet Evol. 2004 Jun;4(2):125-30. (PMID: 15157630)
Appl Environ Microbiol. 1999 Apr;65(4):1578-83. (PMID: 10103253)
Lancet. 2013 Jul 20;382(9888):209-22. (PMID: 23680352)
Trends Parasitol. 2018 Nov;34(11):997-1011. (PMID: 30108020)
Parasit Vectors. 2019 Dec 27;12(1):610. (PMID: 31881926)
Parasitol Res. 2008 Oct;103(5):1177-81. (PMID: 18622625)
Trop Anim Health Prod. 2016 Jan;48(1):199-205. (PMID: 26519146)
Parasitol Int. 2018 Dec;67(6):736-741. (PMID: 30055334)
Acta Parasitol. 2021 Jun;66(2):346-353. (PMID: 32996015)
Trends Parasitol. 2019 Jun;35(6):436-451. (PMID: 31076351)
Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122)
Adv Parasitol. 2021;111:1-73. (PMID: 33482973)
Iran J Parasitol. 2012;7(1):80-4. (PMID: 23133476)
Vet Sci. 2021 Sep 11;8(9):. (PMID: 34564585)
Vet Parasitol. 2015 Apr 30;209(3-4):258-63. (PMID: 25771933)
BMC Vet Res. 2021 Oct 18;17(1):332. (PMID: 34663327)
J Parasit Dis. 2013 Oct;37(2):225-30. (PMID: 24431575)
Infect Genet Evol. 2022 Mar;98:105212. (PMID: 35065302)
One Health. 2020 Dec 24;12:100209. (PMID: 33426263)
Parasit Vectors. 2019 Sep 14;12(1):453. (PMID: 31521186)
Vet Res. 2017 Aug 11;48(1):42. (PMID: 28800747)
Parasitol Res. 2008 Apr;102(5):915-20. (PMID: 18165930)
Exp Parasitol. 2013 Nov;135(3):518-23. (PMID: 24036320)
Parasit Vectors. 2021 Jul 15;14(1):365. (PMID: 34266490)
Appl Environ Microbiol. 2003 Jul;69(7):4302-7. (PMID: 12839819)
Mol Cell Probes. 2003 Oct;17(5):223-5. (PMID: 14580396)
Parasit Vectors. 2014 Jul 11;7:321. (PMID: 25015671)
Iran J Parasitol. 2016 Apr-Jun;11(2):195-203. (PMID: 28096853)
Parasite. 2012 Feb;19(1):71-5. (PMID: 22314242)
Trop Biomed. 2018 Sep 1;35(3):724-735. (PMID: 33601759)
Parasitology. 2000 May;120 ( Pt 5):457-64. (PMID: 10840975)
J Parasit Dis. 2019 Dec;43(4):607-615. (PMID: 31749532)
Prev Vet Med. 2022 Jun;203:105632. (PMID: 35427916)
Trop Biomed. 2009 Dec;26(3):267-73. (PMID: 20237440)
Animals (Basel). 2020 Aug 24;10(9):. (PMID: 32846897)
Comp Immunol Microbiol Infect Dis. 2019 Dec;67:101346. (PMID: 31550674)
J Vet Med Sci. 2014 Dec;76(12):1569-75. (PMID: 25649937)
Onderstepoort J Vet Res. 2006 Dec;73(4):269-74. (PMID: 17283727)
فهرسة مساهمة: Keywords: Zoonoses; epidemiology; genotyping; microsporidia; protists; transmission
تواريخ الأحداث: Date Created: 20230508 Latest Revision: 20231102
رمز التحديث: 20231102
مُعرف محوري في PubMed: PMC10157078
DOI: 10.3389/fvets.2023.1139388
PMID: 37152690
قاعدة البيانات: MEDLINE
الوصف
تدمد:2297-1769
DOI:10.3389/fvets.2023.1139388