دورية أكاديمية
The Subtype Identity of Testicular Cancer Cells Determines Their Immunostimulatory Activity in a Coculture Model.
| العنوان: | The Subtype Identity of Testicular Cancer Cells Determines Their Immunostimulatory Activity in a Coculture Model. |
|---|---|
| المؤلفون: | Gayer FA; Institute for Cellular and Molecular Immunology, University Medical Center Göttingen, 37073 Göttingen, Germany.; Clinic of Urology, University Medical Center Göttingen, 37075 Göttingen, Germany., Henkel M; Institute for Cellular and Molecular Immunology, University Medical Center Göttingen, 37073 Göttingen, Germany., Luft J; Institute for Cellular and Molecular Immunology, University Medical Center Göttingen, 37073 Göttingen, Germany., Reichardt SD; Institute for Cellular and Molecular Immunology, University Medical Center Göttingen, 37073 Göttingen, Germany., Fichtner A; Institute of Pathology, University Medical Center Göttingen, 37075 Göttingen, Germany., Legler TJ; Department of Transfusion Medicine, University Medical Center Göttingen, 37075 Göttingen, Germany., Reichardt HM; Institute for Cellular and Molecular Immunology, University Medical Center Göttingen, 37073 Göttingen, Germany. |
| المصدر: | Cancers [Cancers (Basel)] 2023 May 05; Vol. 15 (9). Date of Electronic Publication: 2023 May 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101526829 Publication Model: Electronic Cited Medium: Print ISSN: 2072-6694 (Print) Linking ISSN: 20726694 NLM ISO Abbreviation: Cancers (Basel) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI |
| مستخلص: | Testicular germ cell cancer (TGCC) is subdivided into several subtypes. While seminomatous germ cell tumors (SGCT) are characterized by an intensive infiltration of immune cells which constitute a pro-inflammatory tumor micromilieu (TME), immune cells in non-seminomatous germ cell tumors (NSGCT) are differently composed and less abundant. Previously, we have shown that the seminomatous cell line TCam-2 promotes T cell and monocyte activation in a coculture model, resulting in mutual interactions between both cell types. Here we set out to compare this feature of TCam-2 cells with the non-seminomatous cell line NTERA-2. Peripheral blood T cells or monocytes cocultured with NTERA-2 cells failed to secrete relevant amounts of pro-inflammatory cytokines, and significantly downregulated the expression of genes encoding activation markers and effector molecules. In contrast, immune cells cocultured with TCam-2 cells produced IL-2, IL-6 and TNFα, and strongly upregulated the expression of multiple pro-inflammatory genes. Furthermore, the expression of genes involved in proliferation, stemness and subtype specification remained unaltered in NTERA-2 cells during coculture with T cells or monocytes, indicating the absence of mutual interactions. Collectively, our findings uncover fundamental differences between SGCT and NSGCT in their capability to generate a pro-inflammatory TME, which possibly impacts the clinical features and prognosis of both TGCC subtypes. |
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| معلومات مُعتمدة: | Scholarship Deutsche Forschungsgemeinschaft; RE 1631/17-1 Deutsche Forschungsgemeinschaft |
| فهرسة مساهمة: | Keywords: NTERA-2; T cell; TCam-2; cytokines; inflammation; monocyte; non-seminoma; seminoma; testicular germ cell cancer; tumor microenvironment |
| تواريخ الأحداث: | Date Created: 20230513 Latest Revision: 20230515 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10177190 |
| DOI: | 10.3390/cancers15092619 |
| PMID: | 37174085 |
| قاعدة البيانات: | MEDLINE |
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