دورية أكاديمية
In-ovo echocardiography for application in cardiovascular research.
| العنوان: | In-ovo echocardiography for application in cardiovascular research. |
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| المؤلفون: | Hegemann N; Department of Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum Der Charité (DHZC), Augustenburger Platz 1, 13353, Berlin, Germany.; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany.; German Center for Cardiovascular Research (DZHK), Partner Site Berlin, 10117, Berlin, Germany., Bintig W; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany.; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Biochemistry, Charitéplatz 1, 10117, Berlin, Germany., Perret PL; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany.; German Center for Cardiovascular Research (DZHK), Partner Site Berlin, 10117, Berlin, Germany., Rees J; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany., Viperino A; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany., Eickholt B; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Biochemistry, Charitéplatz 1, 10117, Berlin, Germany., Kuebler WM; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany.; German Center for Cardiovascular Research (DZHK), Partner Site Berlin, 10117, Berlin, Germany., Höpfner M; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany., Nitzsche B; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany.; German Center for Cardiovascular Research (DZHK), Partner Site Berlin, 10117, Berlin, Germany., Grune J; Department of Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum Der Charité (DHZC), Augustenburger Platz 1, 13353, Berlin, Germany. jana.grune@dhzc-charite.de.; Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Physiology, Charitéplatz 1, 10117, Berlin, Germany. jana.grune@dhzc-charite.de.; German Center for Cardiovascular Research (DZHK), Partner Site Berlin, 10117, Berlin, Germany. jana.grune@dhzc-charite.de. |
| المصدر: | Basic research in cardiology [Basic Res Cardiol] 2023 May 16; Vol. 118 (1), pp. 19. Date of Electronic Publication: 2023 May 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Steinkopff Country of Publication: Germany NLM ID: 0360342 Publication Model: Electronic Cited Medium: Internet ISSN: 1435-1803 (Electronic) Linking ISSN: 03008428 NLM ISO Abbreviation: Basic Res Cardiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Darmstadt, Steinkopff. |
| مواضيع طبية MeSH: | Echocardiography* , Heart*, Chick Embryo ; Rats ; Mice ; Animals |
| مستخلص: | Preclinical cardiovascular research relies heavily on non-invasive in-vivo echocardiography in mice and rats to assess cardiac function and morphology, since the complex interaction of heart, circulation, and peripheral organs are challenging to mimic ex-vivo. While n-numbers of annually used laboratory animals worldwide approach 200 million, increasing efforts are made by basic scientists aiming to reduce animal numbers in cardiovascular research according to the 3R's principle. The chicken egg is well-established as a physiological correlate and model for angiogenesis research but has barely been used to assess cardiac (patho-) physiology. Here, we tested whether the established in-ovo system of incubated chicken eggs interfaced with commercially available small animal echocardiography would be a suitable alternative test system in experimental cardiology. To this end, we defined a workflow to assess cardiac function in 8-13-day-old chicken embryos using a commercially available high resolution ultrasound system for small animals (Vevo 3100, Fujifilm Visualsonics Inc.) equipped with a high frequency probe (MX700; centre transmit: 50 MHz). We provide detailed standard operating procedures for sample preparation, image acquisition, data analysis, reference values for left and right ventricular function and dimensions, and inter-observer variabilities. Finally, we challenged incubated chicken eggs with two interventions well-known to affect cardiac physiology-metoprolol treatment and hypoxic exposure-to demonstrate the sensitivity of in-ovo echocardiography. In conclusion, in-ovo echocardiography is a feasible alternative tool for basic cardiovascular research, which can easily be implemented into the small animal research environment using existing infrastructure to replace mice and rat experiments, and thus, reduce use of laboratory animals according to the 3R principle. (© 2023. The Author(s).) |
| References: | Am J Respir Crit Care Med. 2011 Jan 15;183(2):268-76. (PMID: 20709819) J Cardiovasc Dev Dis. 2016 Apr 05;3(2):. (PMID: 29367563) J Anat. 2019 Oct;235(4):794-802. (PMID: 31148176) Dis Model Mech. 2011 May;4(3):411-20. (PMID: 21183476) J Am Soc Echocardiogr. 2017 Dec;30(12):1239-1250.e2. (PMID: 29066223) Eur Heart J Cardiovasc Imaging. 2012 Apr;13(4):309-16. (PMID: 22087011) Mol Oncol. 2019 Dec;13(12):2679-2696. (PMID: 31583820) Am J Physiol Regul Integr Comp Physiol. 2004 Dec;287(6):R1399-406. (PMID: 15319215) Cardiovasc Ultrasound. 2018 Jul 3;16(1):10. (PMID: 29966517) Dev Dyn. 2005 Aug;233(4):1217-37. (PMID: 15986452) Proc Soc Exp Biol Med. 1967 Nov;126(2):507-10. (PMID: 6079932) Comp Med. 2019 May 1;69(3):184-203. (PMID: 31182184) Eur Heart J. 2005 Mar;26(6):607-16. (PMID: 15618026) J Cardiovasc Pharmacol. 2016 May;67(5):402-11. (PMID: 26859196) Angiogenesis. 2022 Feb;25(1):35-45. (PMID: 34905124) PLoS One. 2015 Apr 08;10(4):e0122665. (PMID: 25853735) Eur Heart J Cardiovasc Imaging. 2020 Mar 1;21(3):337-343. (PMID: 31361311) Front Genet. 2019 Mar 01;10:51. (PMID: 30881374) Am J Physiol Heart Circ Physiol. 2003 Oct;285(4):H1563-75. (PMID: 12805021) Circ Cardiovasc Imaging. 2010 Mar;3(2):157-63. (PMID: 20044514) Toxicol In Vitro. 1993 Jul;7(4):541-5. (PMID: 20732249) Br J Cancer. 2010 Jun 29;103(1):18-28. (PMID: 20551952) Front Bioeng Biotechnol. 2019 May 14;7:96. (PMID: 31139625) J Vis Exp. 2009 Sep 16;(31):. (PMID: 19759521) ESC Heart Fail. 2021 Aug;8(4):3130-3144. (PMID: 34002482) J Am Soc Echocardiogr. 2004 Mar;17(3):275-6. (PMID: 14981427) Biotechniques. 2009 Feb;46(2):101-13. (PMID: 19317655) J Appl Physiol (1985). 2011 Oct;111(4):1125-33. (PMID: 21799135) Pulm Circ. 2019 Apr-Jun;9(2):2045894019841978. (PMID: 30880577) Angiogenesis. 2014 Oct;17(4):779-804. (PMID: 25138280) Cardiovasc Ultrasound. 2019 Apr 22;17(1):7. (PMID: 31010431) Pulm Circ. 2019 Apr-Jun;9(2):2045894019841987. (PMID: 30942120) Nature. 2013 Apr 25;496(7446):498-503. (PMID: 23594743) J Am Soc Echocardiogr. 2019 Jan;32(1):1-64. (PMID: 30282592) Cells Tissues Organs. 2022;211(2):222-237. (PMID: 33780951) Fetal Diagn Ther. 2012;32(1-2):22-9. (PMID: 22677618) |
| فهرسة مساهمة: | Keywords: 3R; Alternative methods; Chicken embryo; Echocardiography; Preclinical research |
| تواريخ الأحداث: | Date Created: 20230516 Date Completed: 20230518 Latest Revision: 20231217 |
| رمز التحديث: | 20231217 |
| مُعرف محوري في PubMed: | PMC10188421 |
| DOI: | 10.1007/s00395-023-00989-0 |
| PMID: | 37193927 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1435-1803 |
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| DOI: | 10.1007/s00395-023-00989-0 |