دورية أكاديمية
أعرض في EDS

Papaverine loaded injectable and thermosensitive hydrogel system for improving survival of rat dorsal skin flaps.

التفاصيل البيبلوغرافية
العنوان: Papaverine loaded injectable and thermosensitive hydrogel system for improving survival of rat dorsal skin flaps.
المؤلفون: Ibne Mahbub MS; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, South Korea., Kim YJ; Department of Plastic & Reconstructive Surgery, Soonchunhyang University Hospital, Cheonan, South Korea., Choi H; Department of Plastic & Reconstructive Surgery, Soonchunhyang University Hospital, Cheonan, South Korea. iprskorea@gmail.com.; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, South Korea. iprskorea@gmail.com., Lee BT; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, South Korea. lbt@sch.ac.kr.; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, South Korea. lbt@sch.ac.kr.
المصدر: Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2023 May 20; Vol. 34 (6), pp. 28. Date of Electronic Publication: 2023 May 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 9013087 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4838 (Electronic) Linking ISSN: 09574530 NLM ISO Abbreviation: J Mater Sci Mater Med Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Norwell, MA : Springer
Original Publication: London : Chapman and Hall, c1990-
مواضيع طبية MeSH: Papaverine*/pharmacology , Hydrogels*/pharmacology, Rats ; Animals ; Rats, Sprague-Dawley ; Surgical Flaps/transplantation ; Graft Survival ; Superoxide Dismutase/metabolism
مستخلص: Vasospasm during reconstructive microsurgery is a common, uncertain, and devastating phenomena concerning flap survival. Topical vasodilators as antispasmodic agents are widely used to reduce vasospasm and enhance microvascular anastomosis in reconstructive microsurgery. In this study, thermo-responsive hydrogel (CNH) was fabricated by grafting chitosan (CS) and hyaluronic acid (HA) to poly(N-isopropylacrylamide) (PNIPAM). Papaverine, an anti-spasmodic agent, was then loaded to evaluate its effect on rat skin flap survival. Post-operative flap survival area and water content of rat dorsal skin flap were measured at 7 days after intradermal application of control hydrogel (CNHP0.0) and papaverine loaded hydrogel (CNHP0.4). Tissue malondialdehyde (MDA) content and superoxide dismutase (SOD) activity was measured using enzyme linked immunosorbent assay (ELISA) to determine oxidative stress in flaps. Hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) were performed to evaluate flap angiogenesis and inflammatory markers. Results showed that CNHP0.4 hydrogel could reduce tissue edema (35.63 ± 4.01%), improve flap survival area (76.30 ± 5.39%), increase SOD activity and decrease MDA content. Consequently, it also increased mean vessel density, upregulated expression of CD34 and VEGF, decreased macrophage infiltration, and reduced CD68 and CCR7 expression based on IHC staining. Overall, these results indicate that CNHP0.4 hydrogel can enhance angiogenesis with anti-oxidative and anti-inflammatory effects and promote skin flap survival by preventing vascular spasm.
(© 2023. The Author(s).)
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معلومات مُعتمدة: 2015R1A6A1A03032522 Ministry of Education
المشرفين على المادة: DAA13NKG2Q (Papaverine)
0 (Hydrogels)
EC 1.15.1.1 (Superoxide Dismutase)
تواريخ الأحداث: Date Created: 20230520 Date Completed: 20230522 Latest Revision: 20240919
رمز التحديث: 20240919
مُعرف محوري في PubMed: PMC10199301
DOI: 10.1007/s10856-023-06732-4
PMID: 37209216
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-4838
DOI:10.1007/s10856-023-06732-4