دورية أكاديمية
Aloperine alleviates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome activation.
| العنوان: | Aloperine alleviates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome activation. |
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| المؤلفون: | Zeng J; Department of Physiology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China; Jiangxi Medical College, Shangrao, Jiangxi 334000, China., Liu J; Department of Physiology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China., Huang JH; Department of Pharmacology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China., Fu SP; Jiangxi entai medical equipment co., ltd, China., Wang XY; Department of Physiology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China., Xi C; Department of Pharmacology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China., Cui YR; Department of Physiology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China; Department of Physiology, School of Basic Medicine Science, Central South University, Changsha, Hunan 410078, China. Electronic address: 20070943@jxutcm.edu.cn., Qu F; Department of Pharmacology, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi 330004, China. Electronic address: fei.qu@jxutcm.edu.cn. |
| المصدر: | International immunopharmacology [Int Immunopharmacol] 2023 Jul; Vol. 120, pp. 110142. Date of Electronic Publication: 2023 May 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Inflammasomes*/metabolism , Acute Lung Injury*/chemically induced , Acute Lung Injury*/drug therapy , Acute Lung Injury*/metabolism, Animals ; Mice ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Lipopolysaccharides/adverse effects ; Caspase 1/metabolism ; NF-E2-Related Factor 2 ; Mice, Inbred C57BL |
| مستخلص: | Rationale: Excessive activation of the NLRP3 inflammasome is involved in the pathological progression of acute lung injury (ALI). Aloperine (Alo) has anti-inflammatory effects in many inflammatory disease models; however, its role in ALI remains elusive. In this study, we addressed the role of Alo in NLRP3 inflammasome activation in both ALI mice and LPS-treated RAW264.7 cells. Methods: The activation of the NLRP3 inflammasome in LPS-induced ALI lungs was investigated in C57BL/6 mice. Alo was administered in order to study its effect on NLRP3 inflammasome activation in ALI. RAW264.7 cells were used to evaluate the underlying mechanism of Alo in the activation of the NLRP3 inflammasome in vitro. Results: The activation of the NLRP3 inflammasome occurs in the lungs and RAW264.7 cells under LPS stress. Alo attenuated the pathological injury of lung tissue as well as downregulates the mRNA expression of NLRP3 and pro-caspase-1 in ALI mice and LPS-stressed RAW264.7 cells. The expression of NLRP3, pro-caspase-1, and caspase-1 p10 were also significantly suppressed by Alo in vivo and in vitro. Furthermore, Alo decreased IL-1β and IL-18 release in ALI mice and LPS-induced RAW264.7 cells. In addition, ML385, a Nrf2 inhibitor, weakened the activity of Alo, which inhibited the activation of the NLRP3 inflammasome in vitro. Conclusion: Alo reduces NLRP3 inflammasome activation via the Nrf2 pathway in ALI mice. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acute lung injury; Aloperine; NLRP3; Nrf2 |
| المشرفين على المادة: | 0 (Inflammasomes) 0 (NLR Family, Pyrin Domain-Containing 3 Protein) 0 (Lipopolysaccharides) EC 3.4.22.36 (Caspase 1) 56293-29-9 (aloperine) 0 (NF-E2-Related Factor 2) |
| تواريخ الأحداث: | Date Created: 20230521 Date Completed: 20230616 Latest Revision: 20230616 |
| رمز التحديث: | 20230616 |
| DOI: | 10.1016/j.intimp.2023.110142 |
| PMID: | 37210910 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1705 |
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| DOI: | 10.1016/j.intimp.2023.110142 |