دورية أكاديمية
أعرض في EDS

Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients.

التفاصيل البيبلوغرافية
العنوان: Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients.
المؤلفون: Mankowski RT; Department of Physiology and Aging, University of Florida, Gainesville, FL 32611, USA., Wohlgemuth SE; Department of Physiology and Aging, University of Florida, Gainesville, FL 32611, USA., Bresciani G; Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA., Martin AD; Department of Physical Therapy, University of Florida, Gainesville, FL 32611, USA.; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Arnaoutakis G; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Martin T; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Jeng E; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Ferreira L; Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA., Machuca T; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Rackauskas M; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Smuder AJ; Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA., Beaver T; Department of Surgery, University of Florida, Gainesville, FL 32611, USA., Leeuwenburgh C; Department of Physiology and Aging, University of Florida, Gainesville, FL 32611, USA., Smith BK; Department of Physical Therapy, University of Florida, Gainesville, FL 32611, USA.
المصدر: Antioxidants (Basel, Switzerland) [Antioxidants (Basel)] 2023 Apr 27; Vol. 12 (5). Date of Electronic Publication: 2023 Apr 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101668981 Publication Model: Electronic Cited Medium: Print ISSN: 2076-3921 (Print) Linking ISSN: 20763921 NLM ISO Abbreviation: Antioxidants (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2012]-
مستخلص: Mechanical ventilation during cardiothoracic surgery is life-saving but can lead to ventilator-induced diaphragm dysfunction (VIDD) and prolong ventilator weaning and hospital length of stay. Intraoperative phrenic nerve stimulation may preserve diaphragm force production to offset VIDD; we also investigated changes in mitochondrial function after stimulation. During cardiothoracic surgeries ( n = 21), supramaximal, unilateral phrenic nerve stimulation was performed every 30 min for 1 min. Diaphragm biopsies were collected after the last stimulation and analyzed for mitochondrial respiration in permeabilized fibers and protein expression and enzymatic activity of biomarkers of oxidative stress and mitophagy. Patients received, on average, 6.2 ± 1.9 stimulation bouts. Stimulated hemidiaphragms showed lower leak respiration, maximum electron transport system (ETS) capacities, oxidative phosphorylation (OXPHOS), and spare capacity compared with unstimulated sides. There were no significant differences between mitochondrial enzyme activities and oxidative stress and mitophagy protein expression levels. Intraoperative phrenic nerve electrical stimulation led to an acute decrease of mitochondrial respiration in the stimulated hemidiaphragm, without differences in biomarkers of mitophagy or oxidative stress. Future studies warrant investigating optimal stimulation doses and testing post-operative chronic stimulation effects on weaning from the ventilator and rehabilitation outcomes.
References: Cells. 2020 Dec 02;9(12):. (PMID: 33276460)
Am J Respir Crit Care Med. 2017 Dec 15;196(12):1544-1558. (PMID: 28787181)
EMBO Rep. 2016 Mar;17(3):300-16. (PMID: 26882551)
Nat Protoc. 2008;3(6):965-76. (PMID: 18536644)
J Appl Physiol (1985). 2019 Aug 1;127(2):312-319. (PMID: 31161881)
Free Radic Biol Med. 2015 Jun;83:12-20. (PMID: 25701433)
Crit Care Med. 2014 Sep;42(9):e633-4. (PMID: 25126818)
Crit Care Med. 2014 Feb;42(2):e152-6. (PMID: 24126442)
Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22567-22572. (PMID: 31636180)
Biochemistry. 2013 Apr 23;52(16):2793-809. (PMID: 23547908)
FASEB J. 2015 Aug;29(8):3515-26. (PMID: 25957282)
N Engl J Med. 2016 Sep 8;375(10):e22. (PMID: 27602683)
Anal Biochem. 2016 Mar 1;496:76-8. (PMID: 26706797)
J Physiol. 2012 Jul 15;590(14):3349-60. (PMID: 22586215)
J Appl Physiol (1985). 2015 May 1;118(9):1161-71. (PMID: 25767033)
Thorax. 2011 Feb;66(2):121-7. (PMID: 21097816)
J Cell Biol. 2010 Apr 19;189(2):211-21. (PMID: 20404107)
Am J Respir Crit Care Med. 2018 Jan 15;197(2):204-213. (PMID: 28930478)
Scand Cardiovasc J. 2004 Mar;38(1):46-52. (PMID: 15204247)
J Biol Chem. 2015 Dec 11;290(50):29692-4. (PMID: 26657753)
J Cardiovasc Surg (Torino). 1975 Sep-Oct;16(5):493-9. (PMID: 1194334)
Cell Metab. 2018 Feb 6;27(2):439-449.e5. (PMID: 29337137)
Am J Respir Crit Care Med. 2014 Oct 1;190(7):837-9. (PMID: 25271750)
Am J Physiol Endocrinol Metab. 2021 Jul 1;321(1):E80-E89. (PMID: 34121449)
J Transl Med. 2016 Oct 26;14(1):305. (PMID: 27784315)
PLoS One. 2017 Jan 30;12(1):e0171007. (PMID: 28135290)
J Appl Physiol (1985). 2019 Aug 1;127(2):423-431. (PMID: 31161883)
J Appl Physiol (1985). 2016 Jul 1;121(1):299-311. (PMID: 27283918)
J Appl Physiol (1985). 2018 Apr 1;124(4):1045-1053. (PMID: 29357496)
FASEB J. 2016 Jan;30(1):417-27. (PMID: 26452378)
Perfusion. 2015 Oct;30(7):590-5. (PMID: 25686857)
Med Sci Sports Exerc. 2018 Dec;50(12):2409-2417. (PMID: 30102675)
Eur Rev Med Pharmacol Sci. 2019 Sep;23(18):8115-8123. (PMID: 31599439)
Am J Respir Crit Care Med. 2019 Oct 15;200(8):e70-e88. (PMID: 31613151)
J Cachexia Sarcopenia Muscle. 2018 Oct;9(5):947-961. (PMID: 29978560)
Redox Biol. 2021 Jan;38:101802. (PMID: 33279868)
Eur Respir J. 2019 Jun 13;53(6):. (PMID: 30956204)
معلومات مُعتمدة: R01 HL130318 United States HL NHLBI NIH HHS; R01 AR072328 United States NH NIH HHS
فهرسة مساهمة: Keywords: VIDD; biopsy; cardiothoracic surgery; mitophagy; respiration
تواريخ الأحداث: Date Created: 20230527 Latest Revision: 20230614
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10215949
DOI: 10.3390/antiox12051009
PMID: 37237876
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-3921
DOI:10.3390/antiox12051009