دورية أكاديمية
Plasmonic Bowl-Shaped Nanopore for Raman Detection of Single DNA Molecules in Flow-Through.
| العنوان: | Plasmonic Bowl-Shaped Nanopore for Raman Detection of Single DNA Molecules in Flow-Through. |
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| المؤلفون: | Zhao Y; Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.; Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5 A, 90220 Oulu, Finland., Hubarevich A; Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy., De Fazio AF; Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy., Iarossi M; Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy., Huang JA; Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5 A, 90220 Oulu, Finland., De Angelis F; Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. |
| المصدر: | Nano letters [Nano Lett] 2023 Jun 14; Vol. 23 (11), pp. 4830-4836. Date of Electronic Publication: 2023 Jun 01. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101088070 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6992 (Electronic) Linking ISSN: 15306984 NLM ISO Abbreviation: Nano Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, c2001- |
| مواضيع طبية MeSH: | Nanopores*, DNA/chemistry ; Gold/chemistry ; Nanotechnology/methods ; Amino Acids ; Spectrum Analysis, Raman |
| مستخلص: | Plasmonic nanopores combined with Raman spectroscopy are emerging as platforms for single-molecule detection and sequencing in label-free mode. Recently, the ability of identifying single DNA bases or amino acids has been demonstrated for molecules adsorbed on plasmonic particles and then delivered into the plasmonic pores. Here, we report on bowl-shaped plasmonic gold nanopores capable of direct Raman detection of single λ-DNA molecules in a flow-through scheme. The bowl shape enables the incident laser to be focused into the nanopore to generate a single intense hot spot with no cut off in pore size. Therefore, we achieved ultrasmall focusing of NIR light in a spot of 3 nm. This enabled us to detect 7 consecutive bases along the DNA chain in flow-through conditions. Furthermore, we found a novel electrofluidic mechanism to manipulate the molecular trajectory within the pore volume so that the molecule is pushed toward the hot spot, thus improving the detection efficiency. |
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| فهرسة مساهمة: | Keywords: DNA detection; flow-through; nanofluidic molecular trapping; plasmonic nanopore; surface-enhanced Raman spectroscopy |
| المشرفين على المادة: | 9007-49-2 (DNA) 7440-57-5 (Gold) 0 (Amino Acids) |
| تواريخ الأحداث: | Date Created: 20230601 Date Completed: 20230615 Latest Revision: 20230622 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC10273459 |
| DOI: | 10.1021/acs.nanolett.3c00340 |
| PMID: | 37260351 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1530-6992 |
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| DOI: | 10.1021/acs.nanolett.3c00340 |