دورية أكاديمية
[Therapeutic effect of ursodeoxycholic acid-berberine supramolecular nanoparticles on ulcerative colitis based on supramolecular system induced by weak bond].
| العنوان: | [Therapeutic effect of ursodeoxycholic acid-berberine supramolecular nanoparticles on ulcerative colitis based on supramolecular system induced by weak bond]. |
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| المؤلفون: | Gao S; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Gao F; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Kong JW; School of Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China., Wang ZJ; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Zheng HC; School of Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China., Jiang XQ; School of Life Sciences, Beijing University of Chinese Medicine Beijing 102488, China., Xu SJ; School of Life Sciences, Beijing University of Chinese Medicine Beijing 102488, China., Li SL; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Lu MJ; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Dai ZQ; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Chu FH; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Xu B; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China., Lei HM; School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China. |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2023 May; Vol. 48 (10), pp. 2739-2748. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | Colitis, Ulcerative*/chemically induced , Colitis, Ulcerative*/drug therapy , Berberine*/pharmacology , Drugs, Chinese Herbal*/pharmacology , Nanoparticles* , Colitis*/chemically induced, Animals ; Mice ; Ursodeoxycholic Acid/adverse effects ; Interleukin-6 ; Tumor Necrosis Factor-alpha/pharmacology ; Colon ; Dextran Sulfate/adverse effects ; Disease Models, Animal |
| مستخلص: | Ulcerative colitis(UC) is a recurrent, intractable inflammatory bowel disease. Coptidis Rhizoma and Bovis Calculus, serving as heat-clearing and toxin-removing drugs, have long been used in the treatment of UC. Berberine(BBR) and ursodeoxycholic acid(UDCA), the main active components of Coptidis Rhizoma and Bovis Calculus, respectively, were employed to obtain UDCA-BBR supramolecular nanoparticles by stimulated co-decocting process for enhancing the therapeutic effect on UC. As revealed by the characterization of supramolecular nanoparticles by field emission scanning electron microscopy(FE-SEM) and dynamic light scattering(DLS), the supramolecular nanoparticles were tetrahedral nanoparticles with an average particle size of 180 nm. The molecular structure was described by ultraviolet spectroscopy, fluorescence spectroscopy, infrared spectroscopy, high-resolution mass spectrometry, and hydrogen-nuclear magnetic resonance(H-NMR) spectroscopy. The results showed that the formation of the supramolecular nano-particle was attributed to the mutual electrostatic attraction and hydrophobic interaction between BBR and UDCA. Additionally, supramolecular nanoparticles were also characterized by sustained release and pH sensitivity. The acute UC model was induced by dextran sulfate sodium(DSS) in mice. It was found that supramolecular nanoparticles could effectively improve body mass reduction and colon shortening in mice with UC(P<0.001) and decrease disease activity index(DAI)(P<0.01). There were statistically significant differences between the supramolecular nanoparticles group and the mechanical mixture group(P<0.001, P<0.05). Enzyme-linked immunosorbent assay(ELISA) was used to detect the serum levels of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6), and the results showed that supramolecular nanoparticles could reduce serum TNF-α and IL-6 levels(P<0.001) and exhibited an obvious difference with the mechanical mixture group(P<0.01, P<0.05). Flow cytometry indicated that supramolecular nanoparticles could reduce the recruitment of neutrophils in the lamina propria of the colon(P<0.05), which was significantly different from the mechanical mixture group(P<0.05). These findings suggested that as compared with the mechanical mixture, the supramolecular nanoparticles could effectively improve the symptoms of acute UC in mice. The study provides a new research idea for the poor absorption of small molecules and the unsatisfactory therapeutic effect of traditional Chinese medicine and lays a foundation for the research on the nano-drug delivery system of traditional Chinese medicine. |
| فهرسة مساهمة: | Keywords: berberine; self-assembly; supramolecule; ulcerative colitis; ursodeoxycholic acid |
| المشرفين على المادة: | 724L30Y2QR (Ursodeoxycholic Acid) 0I8Y3P32UF (Berberine) 0 (Interleukin-6) 0 (Tumor Necrosis Factor-alpha) 0 (Drugs, Chinese Herbal) 9042-14-2 (Dextran Sulfate) |
| تواريخ الأحداث: | Date Created: 20230607 Date Completed: 20230608 Latest Revision: 20230608 |
| رمز التحديث: | 20231215 |
| DOI: | 10.19540/j.cnki.cjcmm.20230223.301 |
| PMID: | 37282934 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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| DOI: | 10.19540/j.cnki.cjcmm.20230223.301 |