دورية أكاديمية
Structural changes in the SARS-CoV-2 spike E406W mutant escaping a clinical monoclonal antibody cocktail.
| العنوان: | Structural changes in the SARS-CoV-2 spike E406W mutant escaping a clinical monoclonal antibody cocktail. |
|---|---|
| المؤلفون: | Addetia A; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA, USA; Department of Biochemistry, University of Washington, Seattle, WA, USA., Park YJ; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Starr T; Howard Hughes Medical Institute, Seattle, WA 98195, USA; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA., Greaney AJ; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Sprouse KR; Department of Biochemistry, University of Washington, Seattle, WA, USA., Bowen JE; Department of Biochemistry, University of Washington, Seattle, WA, USA., Tiles SW; Center for Emerging and Re-emerging Infectious Diseases, Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA., Van Voorhis WC; Center for Emerging and Re-emerging Infectious Diseases, Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA., Bloom JD; Howard Hughes Medical Institute, Seattle, WA 98195, USA; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA., Corti D; Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland., Walls AC; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA. Electronic address: dveesler@uw.edu. |
| المصدر: | Cell reports [Cell Rep] 2023 Jun 27; Vol. 42 (6), pp. 112621. Date of Electronic Publication: 2023 May 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
| مواضيع طبية MeSH: | SARS-CoV-2* , COVID-19*, Humans ; Combined Antibody Therapeutics ; Antibodies, Viral ; Antibodies, Neutralizing ; Antibodies, Monoclonal ; Spike Glycoprotein, Coronavirus ; Neutralization Tests |
| مستخلص: | Continued evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is eroding antibody responses elicited by prior vaccination and infection. The SARS-CoV-2 receptor-binding domain (RBD) E406W mutation abrogates neutralization mediated by the REGEN-COV therapeutic monoclonal antibody (mAb) COVID-19 cocktail and the AZD1061 (COV2-2130) mAb. Here, we show that this mutation remodels the receptor-binding site allosterically, thereby altering the epitopes recognized by these three mAbs and vaccine-elicited neutralizing antibodies while remaining functional. Our results demonstrate the spectacular structural and functional plasticity of the SARS-CoV-2 RBD, which is continuously evolving in emerging SARS-CoV-2 variants, including currently circulating strains that are accumulating mutations in the antigenic sites remodeled by the E406W substitution. Competing Interests: Declaration of interests The Veesler laboratory has received a sponsored research agreement from Vir Biotechnology, Inc. J.D.B. consults for Moderna and Flagship Labs 77 on topics related to viral evolution and is an inventor on Fred Hutch licensed patents related to viral deep mutational scanning. D.C. is an employee of Vir Biotechnology, Inc., and may hold shares in Vir Biotechnology, Inc. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| التعليقات: | Update of: bioRxiv. 2022 Jan 25:2022.01.21.477288. doi: 10.1101/2022.01.21.477288. (PMID: 35118471) |
| معلومات مُعتمدة: | R01 GM120553 United States GM NIGMS NIH HHS; DP1 AI158186 United States AI NIAID NIH HHS; United States HHMI Howard Hughes Medical Institute; INV-004949 United States GATES Bill & Melinda Gates Foundation; U01 AI151698 United States AI NIAID NIH HHS; 75N93022C00036 United States AI NIAID NIH HHS; P01 AI167966 United States AI NIAID NIH HHS; T32 GM007270 United States GM NIGMS NIH HHS; R01 AI141707 United States AI NIAID NIH HHS; T32 GM136534 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: COVID-19; CP: Immunology; CP: Microbiology; SARS-CoV-2; cryo-EM; monoclonal antibodies; receptor binding domain; spike glycoprotein |
| المشرفين على المادة: | 0 (Combined Antibody Therapeutics) 0 (Antibodies, Viral) 0 (Antibodies, Neutralizing) 0 (Antibodies, Monoclonal) 0 (Spike Glycoprotein, Coronavirus) 0 (spike protein, SARS-CoV-2) |
| SCR Organism: | SARS-CoV-2 variants |
| تواريخ الأحداث: | Date Created: 20230610 Date Completed: 20231004 Latest Revision: 20240923 |
| رمز التحديث: | 20240923 |
| مُعرف محوري في PubMed: | PMC10213294 |
| DOI: | 10.1016/j.celrep.2023.112621 |
| PMID: | 37300832 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2211-1247 |
|---|---|
| DOI: | 10.1016/j.celrep.2023.112621 |