دورية أكاديمية
Effect from dinoponeratoxin M-PONTXDq3a arginine and lysine substituted analogues against Staphylococcus aureus strains.
| العنوان: | Effect from dinoponeratoxin M-PONTXDq3a arginine and lysine substituted analogues against Staphylococcus aureus strains. |
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| المؤلفون: | Nonato da Silva Júnior P; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil., Serra Nunes JV; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil., Duque BR; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil., Batista Pereira AJ; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil., Magalhães EP; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil., Oliveira CS; Department of Molecular Biology, Federal University of São Paulo, São Paulo, SP, Brazil., Freire KA; Center for Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil., Pedron CN; Center for Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil., Oliveira VX; Center for Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil; Department of Biophyics, Federal University of São Paulo, São Paulo, SP, Brazil., Sampaio TL; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil., Costa Martins AM; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: martinsalice@gmail.com. |
| المصدر: | Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 Aug 01; Vol. 231, pp. 107190. Date of Electronic Publication: 2023 Jun 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1962- |
| مواضيع طبية MeSH: | Staphylococcus aureus* , Methicillin-Resistant Staphylococcus aureus*, Lysine/pharmacology ; Arginine/pharmacology ; Anti-Bacterial Agents/pharmacology ; Peptides/chemistry ; Microbial Sensitivity Tests ; Amino Acids |
| مستخلص: | The growing incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections is associated with increased mortality rates, which has generated interest in the development of antimicrobial peptides (AMP), such as those found in the giant ant Dinoponera quadríceps. In order to improve the net positive charge and the antibacterial activity of the AMP, amino acids with positive side chain single substituted analogues have been proposed, mainly arginine or lysine. The present work aims to study the antimicrobial activity of the analogues of M-PONTX-Dq3a, a 23 amino acid AMP identified in the D. quadriceps venom. M-PONTX-Dq3a[1-15], a fragment containing the 15 central amino acids, and eight derivatives of single arginine or lysine substituted analogues were proposed. The antimicrobial activity of peptides was evaluated against Staphylococcus aureus ATCC 6538 P (MSSA) and ATCC 33591 (MRSA) strains, followed by minimum inhibitory concentration (MIC), minimum lethal concentration (MLC), and minimum biofilm inhibitory concentration (MBIC) measurement. The membrane permeability was then assessed via crystal violet assay and flow cytometry analysis. The effect of exposure time on microbial viability (Time-Kill) was evaluated. Finally, ultrastructural alterations were evaluated through scanning electron microscopy (SEM). Both arginine-substituted peptides [Arg] 3 M-PONTX-Dq3a[1-15] and [Arg] 4 M-PONTX-Dq3a[1-15], showed lowest MIC and MLC values (each 0.78 μM). In the biofilm formation assays, the peptide [Arg] 3 M-PONTX-Dq3a [1-15] showed MBIC of 3.12 μM against the two tested strains. Both peptides were able to alter the membrane permeability approximately by 80%. The treatment with MIC was able to eliminate bacteria after 2 h of contact on the other hand, treatment with half of the MIC, the population of both bacterial strains remained constant for up to 12 h, indicating a possible bacteriostatic effect. The SEM results showed that the treatment with the lowest concentration (0.78 μM) of both peptides caused disruption of the cell membrane, destabilization of the intercellular interaction and the CLM of [Arg] 4 M-PONTX-Dq3a [1-15] resulted in the complete eradication of the bacteria. Thus, this study describes two AMPs active against MSSA and MRSA and also inhibits the biofilm formation of these stains. This study finds [Arg] 3 M-PONTX-Dq3a[1-15] and [Arg] 4 M-PONTX-Dq3a[1-15] as alternative substances to treat resistant and/or biofilm-forming strains. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial peptides; Biofilm; Dinoponera quadriceps; Methicillin-resistant |
| المشرفين على المادة: | K3Z4F929H6 (Lysine) 94ZLA3W45F (Arginine) 0 (methyl 2-(-5-fluoro-2-hydroxyphenyl)-1 H-benzo(d)imidazole-5-carboxylate) 0 (Anti-Bacterial Agents) 0 (Peptides) 0 (Amino Acids) |
| تواريخ الأحداث: | Date Created: 20230610 Date Completed: 20230814 Latest Revision: 20230814 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.toxicon.2023.107190 |
| PMID: | 37301297 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3150 |
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| DOI: | 10.1016/j.toxicon.2023.107190 |