دورية أكاديمية
Cytological and functional effect of complement 3a on Human Scleral Fibroblasts.
| العنوان: | Cytological and functional effect of complement 3a on Human Scleral Fibroblasts. |
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| المؤلفون: | Xiao K; Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R.China., Jie Y; Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmic and Visual Science Key Lab, Beijing, P.R. China., Luo M; Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R.China., Long Q; Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R.China. |
| المصدر: | Cutaneous and ocular toxicology [Cutan Ocul Toxicol] 2023 Sep; Vol. 42 (3), pp. 137-143. Date of Electronic Publication: 2023 Jun 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 101266892 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1556-9535 (Electronic) Linking ISSN: 15569527 NLM ISO Abbreviation: Cutan Ocul Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: Philadelphia, PA : Taylor & Francis, Inc., c2005- |
| مواضيع طبية MeSH: | Matrix Metalloproteinase 2*/metabolism , Matrix Metalloproteinase 2*/pharmacology , Complement C3a*/metabolism , Complement C3a*/pharmacology, Humans ; Collagen Type I/metabolism ; Collagen Type I/pharmacology ; Fibroblasts ; Apoptosis |
| مستخلص: | Purpose: The complement system is considered to play an important role in the progression of myopia, whereas the influence of complement activation on the human scleral fibroblasts (HSFs) remains unknown. Hence, the effect of complement 3a (C3a) on HSFs was investigated in this study. Methods: HSFs were cultured with exogenous C3a at 0.1 μM for various periods following different measurement protocols, and cells without C3a treatment served as negative control (NC). Cell viability was investigated using the MTS assay after 3 days of C3a treatment. Cell proliferation was evaluated by the 5-Ethynyl-20-Deoxyuridine (EdU) assay following C3a stimulation for 24 hours. Apoptosis was assessed by Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining following C3a stimulation for 48 hours and the stained cells were analysed using flow cytometry. The levels of type I collagen and matrix metalloproteinase-2 (MMP-2) were analysed using ELISA following C3a stimulation for 36 and 60 hours. The level of CD59 were analysed using western blot following C3a stimulation for 60 hours. Results: The MTS assay revealed that cell viability was attenuated by 13% and 8% after C3a for 2 and 3 days, respectively ( P < 0.05). The EdU assay demonstrated a 9% decrease in proliferation rate for the C3a-treated cells after 24 hours ( P < 0.05). The apoptosis analysis revealed an increased percentage of cells in early apoptosis ( P = 0.02) and total apoptosis ( P = 0.02) in the C3a-treated group. Compared with NC group, the level of MMP-2 was increased by 17.6% ( P = 0.002), whereas the levels of type I collagen and CD59 were respectively decreased by 12.5% ( P = 0.024) and 21.6% ( P = 0.044) with C3a treatment for 60 hours. Conclusions: These results indicated that C3a-induced complement activation is potentially involved in inducing myopic-associated scleral extracellular matrix remodelling via mediating the proliferation and function of HSFs. |
| فهرسة مساهمة: | Keywords: Complement 3a; human scleral fibroblasts; myopia |
| المشرفين على المادة: | EC 3.4.24.24 (Matrix Metalloproteinase 2) 80295-42-7 (Complement C3a) 0 (Collagen Type I) |
| تواريخ الأحداث: | Date Created: 20230619 Date Completed: 20230809 Latest Revision: 20230809 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1080/15569527.2023.2226711 |
| PMID: | 37335830 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1556-9535 |
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| DOI: | 10.1080/15569527.2023.2226711 |