دورية أكاديمية
أعرض في EDS

Pressure pushes tRNA Lys3 into excited conformational states.

التفاصيل البيبلوغرافية
العنوان: Pressure pushes tRNA Lys3 into excited conformational states.
المؤلفون: Wang J; Graduate Program in Biochemistry and Biophysics, Rensselaer Polytechnic Institute, Troy, NY 12180., Koduru T; Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180., Harish B; Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180., McCallum SA; Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180., Larsen KP; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305., Patel KS; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305., Peters EV; Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180., Gillilan RE; Cornell High Energy Synchrotron Source, Cornell University, Ithaca, NY 14853., Puglisi EV; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305., Puglisi JD; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305., Makhatadze G; Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180., Royer CA; Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180.
المصدر: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Jun 27; Vol. 120 (26), pp. e2215556120. Date of Electronic Publication: 2023 Jun 20.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : National Academy of Sciences
مواضيع طبية MeSH: RNA* , Anticodon*, Nucleic Acid Conformation ; Scattering, Small Angle ; X-Ray Diffraction ; RNA, Transfer, Lys/chemistry
مستخلص: Conformational dynamics play essential roles in RNA function. However, detailed structural characterization of excited states of RNA remains challenging. Here, we apply high hydrostatic pressure (HP) to populate excited conformational states of tRNA Lys3 , and structurally characterize them using a combination of HP 2D-NMR, HP-SAXS (HP-small-angle X-ray scattering), and computational modeling. HP-NMR revealed that pressure disrupts the interactions of the imino protons of the uridine and guanosine U-A and G-C base pairs of tRNA Lys3 . HP-SAXS profiles showed a change in shape, but no change in overall extension of the transfer RNA (tRNA) at HP. Configurations extracted from computational ensemble modeling of HP-SAXS profiles were consistent with the NMR results, exhibiting significant disruptions to the acceptor stem, the anticodon stem, and the D-stem regions at HP. We propose that initiation of reverse transcription of HIV RNA could make use of one or more of these excited states.
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معلومات مُعتمدة: P30 GM124166 United States GM NIGMS NIH HHS; P50 AI150464 United States AI NIAID NIH HHS; U54 AI170856 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: MD simulations; NMR; RNA; SAXS; high pressure
المشرفين على المادة: 63231-63-0 (RNA)
0 (Anticodon)
0 (RNA, Transfer, Lys)
تواريخ الأحداث: Date Created: 20230620 Date Completed: 20230622 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10293818
DOI: 10.1073/pnas.2215556120
PMID: 37339210
قاعدة البيانات: MEDLINE
الوصف
تدمد:1091-6490
DOI:10.1073/pnas.2215556120