دورية أكاديمية
أعرض في EDS

A malaria parasite phospholipase facilitates efficient asexual blood stage egress.

التفاصيل البيبلوغرافية
العنوان: A malaria parasite phospholipase facilitates efficient asexual blood stage egress.
المؤلفون: Ramaprasad A; Malaria Biochemistry Laboratory, The Francis Crick Institute, London, United Kingdom., Burda PC; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany., Koussis K; Malaria Biochemistry Laboratory, The Francis Crick Institute, London, United Kingdom., Thomas JA; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom., Pietsch E; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany., Calvani E; Metabolomics Science Technology Platform, The Francis Crick Institute, London, United Kingdom., Howell SA; Proteomics Science Technology Platform, The Francis Crick Institute, London, United Kingdom., MacRae JI; Metabolomics Science Technology Platform, The Francis Crick Institute, London, United Kingdom., Snijders AP; Proteomics Science Technology Platform, The Francis Crick Institute, London, United Kingdom., Gilberger TW; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany., Blackman MJ; Malaria Biochemistry Laboratory, The Francis Crick Institute, London, United Kingdom.; Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
المصدر: PLoS pathogens [PLoS Pathog] 2023 Jun 23; Vol. 19 (6), pp. e1011449. Date of Electronic Publication: 2023 Jun 23 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Parasites*/metabolism , Malaria* , Malaria, Falciparum*/parasitology, Animals ; Phospholipases ; Perforin ; Proteomics ; Erythrocytes/parasitology ; Plasmodium falciparum/metabolism ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism
مستخلص: Malaria parasite release (egress) from host red blood cells involves parasite-mediated membrane poration and rupture, thought to involve membrane-lytic effector molecules such as perforin-like proteins and/or phospholipases. With the aim of identifying these effectors, we disrupted the expression of two Plasmodium falciparum perforin-like proteins simultaneously and showed that they have no essential roles during blood stage egress. Proteomic profiling of parasite proteins discharged into the parasitophorous vacuole (PV) just prior to egress detected the presence in the PV of a lecithin:cholesterol acyltransferase (LCAT; PF3D7_0629300). Conditional ablation of LCAT resulted in abnormal egress and a reduced replication rate. Lipidomic profiles of LCAT-null parasites showed drastic changes in several phosphatidylserine and acylphosphatidylglycerol species during egress. We thus show that, in addition to its previously demonstrated role in liver stage merozoite egress, LCAT is required to facilitate efficient egress in asexual blood stage malaria parasites.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Ramaprasad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
References: Mol Microbiol. 2013 May;88(4):687-701. (PMID: 23489321)
EMBO J. 2009 Mar 18;28(6):725-35. (PMID: 19214190)
Blood. 2011 Apr 14;117(15):4118-24. (PMID: 21297002)
Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):3439-3444. (PMID: 28292906)
Nat Methods. 2015 Jun;12(6):568-76. (PMID: 25915120)
Curr Biol. 2005 Sep 20;15(18):1645-50. (PMID: 16169486)
EMBO Rep. 2019 Jul;20(7):e47055. (PMID: 31267706)
EMBO Rep. 2019 Jun;20(6):. (PMID: 31097469)
Infect Immun. 2011 Nov;79(11):4523-32. (PMID: 21896773)
Cell Host Microbe. 2015 Oct 14;18(4):433-44. (PMID: 26468747)
mBio. 2018 Jul 3;9(4):. (PMID: 29970464)
J Biol Chem. 2006 Feb 24;281(8):5197-208. (PMID: 16321976)
J Cell Sci. 1996 Aug;109 ( Pt 8):2121-31. (PMID: 8856508)
Cell Host Microbe. 2015 Nov 11;18(5):593-603. (PMID: 26607162)
Nat Methods. 2017 Apr;14(4):450-456. (PMID: 28288121)
mSphere. 2018 Feb 28;3(1):. (PMID: 29507893)
Nat Commun. 2013;4:1736. (PMID: 23591903)
Cell Microbiol. 2018 Oct;20(10):e12868. (PMID: 29900649)
Cell Host Microbe. 2015 Sep 9;18(3):371-81. (PMID: 26355219)
Mol Biol Cell. 1997 Nov;8(11):2233-40. (PMID: 9362065)
Nat Microbiol. 2018 Apr;3(4):447-455. (PMID: 29459732)
Nat Biotechnol. 2014 Aug;32(8):819-21. (PMID: 24880488)
J Biol Chem. 2005 Feb 25;280(8):6752-60. (PMID: 15590623)
Science. 2017 Oct 27;358(6362):518-522. (PMID: 29074774)
PLoS Pathog. 2013 May;9(5):e1003344. (PMID: 23675297)
Nat Rev Microbiol. 2016 Feb;14(2):77-92. (PMID: 26639780)
Cell Host Microbe. 2013 May 15;13(5):521-534. (PMID: 23684304)
Nat Rev Mol Cell Biol. 2006 Jan;7(1):9-19. (PMID: 16365634)
mBio. 2018 Feb 27;9(1):. (PMID: 29487234)
Protein Eng. 2001 Sep;14(9):691-8. (PMID: 11707616)
Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1963-8. (PMID: 24449881)
Cell Microbiol. 2005 Feb;7(2):199-208. (PMID: 15659064)
PLoS One. 2016 Jun 22;11(6):e0157873. (PMID: 27332706)
Cell Host Microbe. 2009 Jul 23;6(1):81-90. (PMID: 19616767)
Toxins (Basel). 2017 Aug 29;9(9):. (PMID: 28850082)
FEMS Microbiol Lett. 1997 Jun 15;151(2):125-30. (PMID: 9228743)
Mol Biochem Parasitol. 2004 Jan;133(1):15-26. (PMID: 14668008)
Mol Biochem Parasitol. 1983 Mar;7(3):247-65. (PMID: 6350871)
Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17498-17508. (PMID: 31413195)
J Biol Chem. 2002 Dec 6;277(49):47524-32. (PMID: 12228245)
Cell. 2007 Dec 14;131(6):1072-83. (PMID: 18083098)
BMC Genomics. 2011 Nov 30;12:587. (PMID: 22129310)
Eukaryot Cell. 2009 Dec;8(12):1869-79. (PMID: 19820120)
PLoS Pathog. 2015 Mar 18;11(3):e1004760. (PMID: 25786000)
Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):531-6. (PMID: 23267069)
PLoS Pathog. 2005 Nov;1(3):241-51. (PMID: 16322767)
PLoS Pathog. 2017 Jul 6;13(7):e1006453. (PMID: 28683142)
J Biol Chem. 2012 Nov 2;287(45):37949-63. (PMID: 22984267)
Int J Med Microbiol. 2018 Jan;308(1):13-24. (PMID: 28784333)
Sci Rep. 2016 Feb 19;6:21800. (PMID: 26892670)
J Biol Chem. 2003 Sep 26;278(39):37658-63. (PMID: 12857731)
Infect Immun. 2011 Mar;79(3):1086-97. (PMID: 21220481)
J Cell Biol. 2003 Jul 21;162(2):317-27. (PMID: 12876279)
Cell Rep. 2017 Mar 28;18(13):3105-3116. (PMID: 28355563)
Science. 2018 May 4;360(6388):. (PMID: 29724925)
Science. 2017 Oct 27;358(6362):522-528. (PMID: 29074775)
Mol Microbiol. 2001 Jul;41(1):47-58. (PMID: 11454199)
Life Sci Alliance. 2020 Mar 16;3(4):. (PMID: 32179592)
Nat Rev Microbiol. 2008 Feb;6(2):99-110. (PMID: 18197167)
Science. 2019 Jun 28;364(6447):1279-1282. (PMID: 31249058)
Cell Microbiol. 2013 Aug;15(8):1438-55. (PMID: 23461714)
Cell Microbiol. 2014 May;16(5):709-33. (PMID: 24602217)
J Biol Chem. 2016 Feb 19;291(8):3725-46. (PMID: 26694607)
Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7509-14. (PMID: 10861015)
Front Cell Infect Microbiol. 2020 Mar 24;10:121. (PMID: 32266171)
Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834)
Sci Rep. 2017 Jun 20;7(1):3881. (PMID: 28634346)
BMC Bioinformatics. 2017 Jul 10;18(1):331. (PMID: 28693421)
Curr Biol. 2010 Jun 22;20(12):1117-21. (PMID: 20537541)
Science. 1976 Aug 20;193(4254):673-5. (PMID: 781840)
Traffic. 2003 Apr;4(4):214-21. (PMID: 12694560)
معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; CC2129 United Kingdom ARC_ Arthritis Research UK; 220318/A/20/Z United Kingdom WT_ Wellcome Trust; United Kingdom CRUK_ Cancer Research UK; United Kingdom MRC_ Medical Research Council
المشرفين على المادة: EC 3.1.- (Phospholipases)
126465-35-8 (Perforin)
0 (Protozoan Proteins)
تواريخ الأحداث: Date Created: 20230623 Date Completed: 20230710 Latest Revision: 20230718
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC10325081
DOI: 10.1371/journal.ppat.1011449
PMID: 37352369
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7374
DOI:10.1371/journal.ppat.1011449