دورية أكاديمية
Rapid and accurate method for quantifying busulfan in plasma samples by isocratic liquid chromatography-tandem mass spectrometry (LC-MS/MS).
| العنوان: | Rapid and accurate method for quantifying busulfan in plasma samples by isocratic liquid chromatography-tandem mass spectrometry (LC-MS/MS). |
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| المؤلفون: | Villena-Ortiz Y; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra, Spain., Castellote-Bellés L; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Martinez-Sanchez L; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra, Spain., Benítez-Carabante MI; Department of Pediatric Hematology and Oncology, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Miarons M; Pharmacy Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Vima-Bofarull J; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Barquin-DelPino R; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Paciucci R; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Rodríguez-Frías F; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain.; Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra, Spain., Ferrer-Costa R; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., Casis E; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain., López-Hellín J; Biochemistry Department, Vall d'Hebron Institut de Recerca (VHIR), Vall d'Hebron University Hospital, Barcelona, Spain. |
| المصدر: | Advances in laboratory medicine [Adv Lab Med] 2022 Jun 13; Vol. 3 (3), pp. 263-281. Date of Electronic Publication: 2022 Jun 13 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English; Spanish; Castilian |
| بيانات الدورية: | Publisher: De Gruyter Country of Publication: Germany NLM ID: 9918284273306676 Publication Model: eCollection Cited Medium: Internet ISSN: 2628-491X (Electronic) Linking ISSN: 2628491X NLM ISO Abbreviation: Adv Lab Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, Germany : De Gruyter, [2020]- |
| مستخلص: | Objectives: Administration of busulfan is extending rapidly as a part of a conditioning regimen in patients undergoing hematopoietic stem cell transplantation (HSCT). Monitoring blood plasma levels of busulfan is recommended for identifying the optimal dose in patients and for minimizing toxicity. The aim of this research was to validate a simple, rapid, and cost-effective analytical tool for measuring busulfan in human plasma that would be suitable for routine clinical use. This novel tool was based on liquid chromatography coupled to mass spectrometry. Methods: Human plasma samples were prepared using a one-step protein precipitation protocol. These samples were then resolved by isocratic elution in a C18 column. The mobile phase consisted 2 mM ammonium acetate and 0.1% formic acid dissolved in a 30:70 ratio of methanol/water. Busulfan-d Results: The run time was optimized at 1.6 min. Standard curves were linear from 0.03 to 5 mg/L. The coefficient of variation (%CV) was less than 8%. The accuracy of this method had an acceptable bias that fell within 85-115% range. No interference between busulfan and the interfering compound hemoglobin, lipemia, or bilirubin not even at the highest concentrations of compound was tested. Neither carryover nor matrix effects were observed using this method. The area under the plasma drug concentration-time curves obtained for 15 pediatric patients who received busulfan therapy prior to HSCT were analyzed and correlated properly with the administered doses. Conclusions: This method was successfully validated and was found to be robust enough for therapeutic drug monitoring in a clinical setting. Competing Interests: Competing interests: Authors state no conflict of interest. (© 2022 Yolanda Villena-Ortiz et al., published by De Gruyter, Berlin/Boston.) |
| References: | Curr Drug Saf. 2008 Jan;3(1):60-6. (PMID: 18690982) J Chromatogr B Biomed Sci Appl. 1997 May 9;692(2):437-44. (PMID: 9188834) Ther Drug Monit. 2014 Apr;36(2):169-74. (PMID: 24625541) Methods Mol Biol. 2016;1383:79-87. (PMID: 26660176) Biol Blood Marrow Transplant. 2016 Mar;22(3):400-9. (PMID: 26431626) Bone Marrow Transplant. 2005 Nov;36(10):839-45. (PMID: 16151422) Ann Lab Med. 2014 Jan;34(1):7-14. (PMID: 24422189) Bone Marrow Transplant. 2003 Nov;32(10):979-86. (PMID: 14595385) Biol Blood Marrow Transplant. 2002;8(9):477-85. (PMID: 12374452) J Pharm Biomed Anal. 2014 Mar;90:207-14. (PMID: 24389463) Nature. 1959 May 30;183(4674):1509-10. (PMID: 13666786) Eur J Haematol. 2021 Mar;106(3):408-416. (PMID: 33296531) J Chromatogr B Biomed Sci Appl. 1999 Jun 25;730(1):33-40. (PMID: 10437669) Bioanalysis. 2012 May;4(8):865-8. (PMID: 22533559) Clin Pharmacokinet. 2012 May 1;51(5):331-45. (PMID: 22455797) J Pharm Biomed Anal. 2016 Feb 20;120:168-74. (PMID: 26736033) Clin Drug Investig. 2012 Sep 1;32(9):641-8. (PMID: 22877323) Sci Rep. 2020 Jun 2;10(1):8913. (PMID: 32488110) Anal Chem. 2003 Jul 1;75(13):3019-30. (PMID: 12964746) J Clin Pharmacol. 2007 Jan;47(1):101-11. (PMID: 17192508) Clin Chim Acta. 2018 Apr;479:190-195. (PMID: 29341904) Methods Mol Biol. 2010;603:129-36. (PMID: 20077065) Paediatr Drugs. 2007;9(4):271-8. (PMID: 17705566) Biol Blood Marrow Transplant. 2017 Mar;23(3):467-474. (PMID: 27916512) J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jun 15;1055-1056:81-85. (PMID: 28445850) Clin Biochem Rev. 2011 Feb;32(1):5-31. (PMID: 21451775) J Chromatogr. 1988 Mar 18;425(2):303-9. (PMID: 3372644) J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 1;1125:121711. (PMID: 31376591) Biol Blood Marrow Transplant. 2008 Feb;14(2):220-8. (PMID: 18215782) Expert Opin Drug Metab Toxicol. 2009 Aug;5(8):957-69. (PMID: 19611402) Biomed Chromatogr. 2020 Sep;34(9):e4898. (PMID: 32428257) J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Dec 1;878(31):3255-8. (PMID: 21030324) |
| فهرسة مساهمة: | Keywords: hematopoietic stem cell transplantation; mass spectrometry platform; method validation; therapeutic drug monitoring |
| تواريخ الأحداث: | Date Created: 20230626 Latest Revision: 20240919 |
| رمز التحديث: | 20240919 |
| مُعرف محوري في PubMed: | PMC10197276 |
| DOI: | 10.1515/almed-2022-0016 |
| PMID: | 37362141 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2628-491X |
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| DOI: | 10.1515/almed-2022-0016 |