دورية أكاديمية

Serum CRP biomarker detection by using carbon nanotube field-effect transistor (CNT-FET) immunosensor.

التفاصيل البيبلوغرافية
العنوان: Serum CRP biomarker detection by using carbon nanotube field-effect transistor (CNT-FET) immunosensor.
المؤلفون: Rabbani G; IT-medical Fusion Center, 350-27 Gumidae-ro, Gumi-si, Gyeongbuk 39253, Republic of Korea., Khan ME; Department of Chemical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia. Electronic address: mekhan@jazanu.edu.sa., Ahmad E; Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Khan MV; Department of Biosciences, Integral University, Lucknow 226026, India., Ahmad A; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21452, Saudi Arabia., Khan AU; Department of Electrical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia., Ali W; Department of Chemical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia., Zamzami MA; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21452, Saudi Arabia., Bashiri AH; Department of Mechanical Engineering, College of Engineering, Jazan University, P. O. Box 114, Jazan 45142, Saudi Arabia., Zakri W; Department of Mechanical Engineering, College of Engineering, Jazan University, P. O. Box 114, Jazan 45142, Saudi Arabia.
المصدر: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Oct; Vol. 153, pp. 108493. Date of Electronic Publication: 2023 Jun 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100953583 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-562X (Electronic) Linking ISSN: 15675394 NLM ISO Abbreviation: Bioelectrochemistry Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam?] : Elsevier, 2000-
مواضيع طبية MeSH: Nanotubes, Carbon* , Biosensing Techniques*/methods, C-Reactive Protein/analysis ; Immunoassay/methods ; Silicon Dioxide ; Biomarkers
مستخلص: C-reactive protein (CRP) is produced by the liver in response to systemic inflammation caused by bacterial infection, trauma and internal organ failures. CRP serves as a potential biomarker in the precise diagnosis of cardiovascular risk, type-2 diabetes, metabolic syndrome, hypertension and various types of cancers. The pathogenic conditions indicated above are diagnosed by an elevated CRP level in the serum. In this study, we successfully fabricated a highly sensitive and selective carbon nanotube field-effect transistor (CNT-FET) immunosensor for the detection of CRP. The CNTs were deposited on the Si/SiO 2 surface, between source-drain electrodes, afterwards modified with well-known linker PBASE and then anti-CRP was immobilized. This anti-CRP functionalized CNT-FET immunosensor exhibits a wide dynamic detection range (0.01-1000 μg/mL) CRP detection, rapid response time (2-3 min) and low variation (<3 %) which can be delivered as a low-cost and rapid clinical detection technology for the early diagnosis of coronary heart disease (CHD). For the clinical applications, our sensor was tested using CRP fortified serum samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). This CNT-FET immunosensor will be helpful in taking over the complex laboratory-based expensive traditional CRP diagnostic procedures practiced in the hospitals.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Antigen-antibody; C-reactive protein; Carbon nanotubes; Field-effect transistor; Immunosensor
المشرفين على المادة: 0 (Nanotubes, Carbon)
9007-41-4 (C-Reactive Protein)
7631-86-9 (Silicon Dioxide)
0 (Biomarkers)
تواريخ الأحداث: Date Created: 20230701 Date Completed: 20231023 Latest Revision: 20231023
رمز التحديث: 20231215
DOI: 10.1016/j.bioelechem.2023.108493
PMID: 37392576
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-562X
DOI:10.1016/j.bioelechem.2023.108493