التفاصيل البيبلوغرافية
| العنوان: |
The HLA-II immunopeptidome of SARS-CoV-2. |
| المؤلفون: |
Weingarten-Gabbay S, Chen DY, Sarkizova S, Taylor HB, Gentili M, Pearlman LR, Bauer MR, Rice CM, Clauser KR, Hacohen N, Carr SA, Abelin JG, Saeed M, Sabeti PC |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Jun 01. Date of Electronic Publication: 2023 Jun 01. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Targeted synthetic vaccines have the potential to transform our response to viral outbreaks; yet the design of these vaccines requires a comprehensive knowledge of viral immunogens, including T-cell epitopes. Having previously mapped the SARS-CoV-2 HLA-I landscape, here we report viral peptides that are naturally processed and loaded onto HLA-II complexes in infected cells. We identified over 500 unique viral peptides from canonical proteins, as well as from overlapping internal open reading frames (ORFs), revealing, for the first time, the contribution of internal ORFs to the HLA-II peptide repertoire. Most HLA-II peptides co-localized with the known CD4+ T cell epitopes in COVID-19 patients. We also observed that two reported immunodominant regions in the SARS-CoV-2 membrane protein are formed at the level of HLA-II presentation. Overall, our analyses show that HLA-I and HLA-II pathways target distinct viral proteins, with the structural proteins accounting for most of the HLA-II peptidome and non-structural and non-canonical proteins accounting for the majority of the HLA-I peptidome. These findings highlight the need for a vaccine design that incorporates multiple viral elements harboring CD4+ and CD8+ T cell epitopes to maximize the vaccine effectiveness. |
| التعليقات: |
Update in: Cell Rep. 2023 Dec 19;43(1):113596. (PMID: 38117652) |
| معلومات مُعتمدة: |
T32 HG002295 United States HG NHGRI NIH HHS; U24 CA270823 United States CA NCI NIH HHS |
| تواريخ الأحداث: |
Date Created: 20230703 Latest Revision: 20240319 |
| رمز التحديث: |
20240319 |
| مُعرف محوري في PubMed: |
PMC10312465 |
| DOI: |
10.1101/2023.05.26.542482 |
| PMID: |
37398281 |
| قاعدة البيانات: |
MEDLINE |
