دورية أكاديمية
Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications.
| العنوان: | Identification, phylogenetic analysis, and genome mining of the tetracycline-resistant Bacillus thuringiensis strain m401 reveal its potential for biotechnological and biocontrol applications. |
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| المؤلفون: | Alippi AM; Unidad de Bacteriología, Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, cc 31, calle 60 y 119, S/N, 1900 La Plata, Argentina; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICBA), Argentina. Electronic address: alippi@biol.unlp.edu.ar., Lamelza F; Unidad de Bacteriología, Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, cc 31, calle 60 y 119, S/N, 1900 La Plata, Argentina., Torres Tejerizo GA; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-CCT La Plata), Argentina; IBBM (Instituto de Biotecnología y Biología Molecular), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, UNLP, Calles 49 y 115 S/N, 1900 La Plata, Argentina., Abrahamovich E; YPF Tecnología (Y-Tec), Av. del Petróleo S/N entre 129 y 143, 1923 Berisso, Argentina., López AC; Unidad de Bacteriología, Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, cc 31, calle 60 y 119, S/N, 1900 La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-CCT La Plata), Argentina. |
| المصدر: | Revista Argentina de microbiologia [Rev Argent Microbiol] 2023 Oct-Dec; Vol. 55 (4), pp. 317-331. Date of Electronic Publication: 2023 Jul 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Asociación Argentina de Microbiología Country of Publication: Argentina NLM ID: 8002834 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0325-7541 (Print) Linking ISSN: 03257541 NLM ISO Abbreviation: Rev Argent Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Buenos Aires, Asociación Argentina de Microbiología. |
| مواضيع طبية MeSH: | Bacillus thuringiensis*/genetics, Food Microbiology ; Phylogeny ; Bacillus cereus ; Anti-Bacterial Agents/pharmacology ; Tetracycline/metabolism |
| مستخلص: | Bacillus thuringiensis is an entomopathogen belonging to the Bacillus cereus clade. We isolated a tetracycline-resistant strain called m401, recovered it from honey, and identified it as Bacillus thuringiensis sv. kumamotoensis based on the average nucleotide identity calculations (ANIb) comparison and the analysis of the gyrB gene sequences of different B. thuringiensis serovars. Sequences with homology to virulence factors [cytK, nheA, nheB, nheC, hblA, hblB, hblC, hblD, entFM, and inhA] and tetracycline resistance genes [tet(45), tet(V), and tet(M)/tet(W)/tet(O)/tet(S) family] were identified in the bacterial chromosome. The prediction of plasmid-coding regions revealed homolog sequences to the MarR and TetR/AcrR family of transcriptional regulators, toxins, and lantipeptides. The genome mining analysis revealed 12 regions of biosynthetic gene clusters responsible for synthesizing secondary metabolites. We identified biosynthetic gene clusters coding for bacteriocins, siderophores, ribosomally synthesized post-translationally modified peptide products, and non-ribosomal peptide synthetase clusters that provide evidence for the possible use of Bt m401 as a biocontrol agent. Furthermore, Bt m401 showed high inhibition against all Paenibacillus larvae genotypes tested in vitro. In conclusion, Bt m401 owns various genes involved in different biological processes, such as transductional regulators associated with antibiotic resistance, toxins, and antimicrobial peptides with potential biotechnological and biocontrol applications. (Copyright © 2023 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial peptides; Bacillus thuringiensis sv. kumamotoensis; Paenibacillus larvae; Péptidos antimicrobianos; Resistencia a tetraciclina; Tetracycline resistance; tet(45) |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) F8VB5M810T (Tetracycline) |
| تواريخ الأحداث: | Date Created: 20230703 Date Completed: 20231225 Latest Revision: 20231225 |
| رمز التحديث: | 20231225 |
| DOI: | 10.1016/j.ram.2023.05.002 |
| PMID: | 37400312 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0325-7541 |
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| DOI: | 10.1016/j.ram.2023.05.002 |