دورية أكاديمية
[Treatment of rheumatoid arthritis by injection of sinomenine solid lipid nanoparticles under a fluorescence endoscopic laser confocal microscope].
| العنوان: | [Treatment of rheumatoid arthritis by injection of sinomenine solid lipid nanoparticles under a fluorescence endoscopic laser confocal microscope]. |
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| المؤلفون: | Zhang JH; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Wen WL; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Zhang WY; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Liang X; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Sun X; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Xiao Z; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Zhao YQ; School of Basic Medicine,Heilongjiang University of Chinese Medicine Harbin 150040,China., Li AQ; School of Basic Medicine,Heilongjiang University of Chinese Medicine Harbin 150040,China., Zhao PR; School of Basic Medicine,Heilongjiang University of Chinese Medicine Harbin 150040,China., Bai HT; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China., Yang J; School of Basic Medicine,Heilongjiang University of Chinese Medicine Harbin 150040,China., Wang R; School of Pharmacy,Heilongjiang University of Chinese Medicine Harbin 150040,China Key Laboratory of Basic and Applied Research of Northern Medicine,Ministry of Education,Heilongjiang University of Chinese Medicine Harbin 150040,China. |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2023 Jul; Vol. 48 (14), pp. 3786-3792. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | Arthritis, Rheumatoid*/drug therapy , Arthritis, Experimental*/drug therapy, Animals ; Rabbits ; Tumor Necrosis Factor-alpha ; Fluorescence ; Interleukin-1 |
| مستخلص: | A fluorescence endoscopic laser confocal microscope(FELCM) was used to direct the injection of sinomenine solid lipid nanoparticles(Sin-SLN) into the joint, and the in vitro effectiveness of Sin-SLN in the treatment of rheumatoid arthritis(RA) was evaluated. Sin-SLN was prepared with the emulsion evaporation-low temperature curing method. The Sin-SLN prepared under the optimal conditions showed the encapsulation efficiency of 64.79%±3.12%, the drug loading of 3.84%±0.28%, the average particle size of(215.27±4.21) nm, and the Zeta potential of(-32.67±0.84) mV. Moreover, the Sin-SLN demonstrated good stability after sto-rage for 30 days. The rabbit model of RA was established by the subcutaneous injection of ovalbumin and complete Freund's adjuvant. Five groups were designed, including a control group, a model group, a Sin(1.5 mg·kg~(-1)) group, a Sin-SLN(1.5 mg·kg~(-1)) group, and a dexamethasone(positive drug, 1.0 mg·kg~(-1), ig) group. The control group and the model group only received puncture treatment without drug injection. After drug administration, the local skin temperature and knee joint diameter were monitored every day. The knee joint diameter and the local skin temperature were lower in the drug administration groups than in the model group(P<0.05, P<0.01). FELCM recorded the morphological alterations of the cartilage of knee joint. The Sin-SLN group showed compact tissue structure and smooth surface of the cartilage. Enzyme-linked immunosorbent assay(ELISA) was employed to determine the serum le-vels of interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α). The findings revealed that the Sin-SLN group had lower IL-1 and TNF-α levels than the model group(P<0.05, P<0.01). Hematoxylin-eosin(HE) staining was employed to reveal the pathological changes of the synovial tissue, which were significantly mitigated in the Sin-SLN group. The prepared Sin-SLN had uniform particle size and high stability. Through joint injection administration, a drug reservoir was formed. Sin-SLN effectively alleviate joint swelling and cartilage damage of rabbit, down-regulated the expression of inflammatory cytokines, and inhibited the epithelial proliferation and inflammatory cell infiltration of the synovial tissue, demonstrating the efficacy in treating RA. |
| فهرسة مساهمة: | Keywords: depo-sit effect; fluorescence endoscopic laser confocal microscope; rheumatoid arthritis; sinomenine; solid lipid nanoparticles |
| المشرفين على المادة: | 63LT81K70N (sinomenine) 0 (Lipid Nanoparticles) 0 (Tumor Necrosis Factor-alpha) 0 (Interleukin-1) |
| تواريخ الأحداث: | Date Created: 20230720 Date Completed: 20230724 Latest Revision: 20230724 |
| رمز التحديث: | 20231215 |
| DOI: | 10.19540/j.cnki.cjcmm.20230424.301 |
| PMID: | 37475070 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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| DOI: | 10.19540/j.cnki.cjcmm.20230424.301 |