دورية أكاديمية
JNK regulates the Nrf2/NQO1-ARE pathway against Microcystins-Induced oxidative stress in freshwater mussel Cristaria plicata.
| العنوان: | JNK regulates the Nrf2/NQO1-ARE pathway against Microcystins-Induced oxidative stress in freshwater mussel Cristaria plicata. |
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| المؤلفون: | Wu J; Jiangxi Science and Technology Normal University, Nanchang 330013, China. Electronic address: 58997282@qq.com., Lv R; College of Life Science, Nanchang University, Nanchang 330031, China., Qiu L; Jiangxi Science and Technology Normal University, Nanchang 330013, China., Zhang S; Jiangxi Science and Technology Normal University, Nanchang 330013, China., Jiao H; Jiangxi Science and Technology Normal University, Nanchang 330013, China., Wang Y; Jiangxi Science and Technology Normal University, Nanchang 330013, China., Luo S; Jiangxi Science and Technology Normal University, Nanchang 330013, China., Fang H; Jiangxi Science and Technology Normal University, Nanchang 330013, China., Wen C; College of Life Science, Nanchang University, Nanchang 330031, China. Electronic address: cgwen@ncu.edu.cn. |
| المصدر: | Gene [Gene] 2023 Oct 20; Vol. 883, pp. 147653. Date of Electronic Publication: 2023 Jul 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Antioxidant Response Elements* , Unionidae*/genetics, Animals ; NF-E2-Related Factor 2/genetics ; NF-E2-Related Factor 2/metabolism ; Kelch-Like ECH-Associated Protein 1/genetics ; Microcystins/toxicity ; Microcystins/genetics ; Oxidative Stress ; Mitogen-Activated Protein Kinases/genetics |
| مستخلص: | In response to stress, cells can utilize several processes, such as the activation of the Nrf2/Keap1 pathway as a critical regulator of oxidative stress to protect against oxidative damage. C-Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase (MAPK) family, is involved in regulating the NF-E2-related nuclear factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway. NAD(P)H quinone redox enzyme-1 (NQO1), a downstream target gene of the Nrf2 pathway, plays a vital role in removing peroxide and providing resistance to oxidative injury. We found that microcystins (MCs) stimulated CpNrf2 to express and increase anti-oxidative enzyme activities in a previous experiment. In our current study, the full-length cDNAs of JNK and NQO1 from Cristaria plicata (designated CpJNK and CpNQO1) were cloned. The relative levels of CpJNK and CpNQO1 were high in hepatopancreas. Upon MCs induction, the relative level of CpNQO1 was increased, whereas that of CpJNK was decreased significantly. In contrast, CpNrf2 knockdown upregulated the expression of CpJNK mRNA and phosphorylation of CpJNK protein (Cpp-JNK), but inhibited CpNQO1 expression. Additionally, we found that JNK inhibitor SP600125 stimulated expression of CpNQO1 and CpNrf2 upon exposure to MCs, and we further confirmed that CpNrf2 protein combined with the ARE element in CpNQO1 gene promoter in vitro, and increased CpNQO1-ARE-luciferase activity in a CpNrf2-dependent manner. These findings indicated C. plicata effectively alleviated MC-induced oxidative injury through JNK participated in regulating the Nrf2/NQO1-ARE pathway. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: C-Jun N-terminal kinase; Cristaria plicata; Microcystins·; NF-E2-related nuclear factor 2; Quinone redox enzyme-1 |
| المشرفين على المادة: | 0 (NF-E2-Related Factor 2) 0 (Kelch-Like ECH-Associated Protein 1) 0 (Microcystins) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20230721 Date Completed: 20230822 Latest Revision: 20230822 |
| رمز التحديث: | 20230822 |
| DOI: | 10.1016/j.gene.2023.147653 |
| PMID: | 37479096 |
| قاعدة البيانات: | MEDLINE |
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